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Lactobacillus rhamnosus CCFM1068 and application thereof

A technology of Lactobacillus rhamnosus and bacterial agents, applied in the field of microorganisms, can solve the problems of drug dependence, induced intestinal polyps, colonic lesions, etc., achieve strong adsorption capacity, increase the water content of feces, and have wide application prospects

Pending Publication Date: 2019-10-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these laxatives have their individual disadvantages. For example, bulky laxatives generally take several days to work, and are not suitable for patients with colonic weakness and poor intestinal motility; stimulant laxatives contain anthraquinones mostly. Long-term use can cause drug dependence, colon lesions and even intestinal polyps

Method used

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  • Lactobacillus rhamnosus CCFM1068 and application thereof
  • Lactobacillus rhamnosus CCFM1068 and application thereof
  • Lactobacillus rhamnosus CCFM1068 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1: Lactobacillus rhamnosus CCFM1068 has good tolerance to simulated gastrointestinal fluid

[0077] The cryopreserved Lactobacillus rhamnosus CCFM1068 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and subcultured in mMRS medium for 2~ After 3 times, take 1 mL of the culture solution of Lactobacillus rhamnosus CCFM1068, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0h, 0.5h, 1h, and 2h, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0078] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. T...

Embodiment 2

[0084] Example 2: Lactobacillus rhamnosus CCFM1068 has no toxic side effects on BALB / C mice

[0085] Resuspend Lactobacillus rhamnosus CCFM1068 in 2% sucrose solution to make a concentration of 3.0 × 10 9 CFU / mL bacterial suspension. Eight healthy male BALB / C mice with a body weight of about 16-20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0086] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 9 CFU / mL of Lactobacillus rhamnosus CCFM1068 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0087] Table 3 Changes in body weight and death of mice

[0088]

[0089] Note: -: no death of mice

Embodiment 3

[0090] Example 3: Effect of Lactobacillus rhamnosus CCFM1068 on the first black stool in mice with constipation

[0091] Take out the Lactobacillus rhamnosus CCFM1068 strain from the -80°C refrigerator, streak it on the MRS plate, culture it at 37°C for 48 hours, pick a single colony in the MRS liquid tube, and culture it at 37°C for 18 hours. Inoculate in new MRS liquid medium, culture at 37°C for 18h, and culture for another generation in the same way, then centrifuge the lactic acid bacteria suspension at 8000r / min, 4°C for 8min, and then use 3% sucrose solution Resuspended to obtain a bacterial suspension and stored in a -80°C refrigerator.

[0092] Thirty 6-week-old healthy male Balb / C mice were taken, adapted to the environment for 1 week, and randomly divided into 5 groups: blank control group (NC), model control group (M), phenolphthalein control group (RH), rhamnose Lactobacillus CCFM1068 intervention group (CCFM1060), Lactobacillus rhamnosus 4-1 control group (4-1),...

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Abstract

The invention discloses lactobacillus rhamnosus CCFM1068 and application thereof.Thelactobacillus rhamnosus CCFM1068 can rapidly colonize in an intestinal tract, the water content of feces issignificantly increased, the intestinal propulsive rate is increased, defecation time of a first black stool is shortened, the content of peptide YYin serum is down-regulated, the level of5-hydroxytryptamine in colon tissuesis up-regulated, the level of neurotrophin-3 in thecolon tissues is up-regulated, the level of brain-derived neurotrophic factorsin thecolon tissues is up-regulated, and the level of Aqp4 gene transcriptionin thecolon tissues is up-regulated. In addition, the lactobacillus rhamnosus CCFM1068has a strong adsorption capacity for perfluorooctanoic acid and the ability to alleviate thetoxicity of PFOA. The lactobacillus rhamnosus CCFM1068 can well tolerate simulated gastrointestinal fluid, be used for preparing pharmaceutical compositions and fermented foods for relieving constipation and PFOA toxicity, and has a very broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Lactobacillus rhamnosus CCFM1068 and application thereof. Background technique [0002] In recent years, with the continuous development of my country's social and economic level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased, and the proportion of crude fiber has gradually decreased. Changes in dietary structure have gradually changed people's intestinal flora. Studies have shown that there are significant differences in the intestinal flora of the constipated population and the normal population. At present, the intervention of probiotics on intestinal flora has become a research hotspot, so it can relieve constipation by mediating intestinal flora. [0003] Constipation has many symptoms, the main manifestations are: defecation effort, hard stool, feeling of wantin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23C11/10A23L11/00A23L19/00A23L33/135A61K35/747A61P31/04C12R1/225
CPCC12N1/20A23L33/135A23L19/00A23C9/1234A23C11/106A61K35/747A61P31/04A23V2002/00A23L11/50C12R2001/225C12N1/205A23V2400/175A23V2200/32
Inventor 陈卫王刚杨树荣赵建新张灏
Owner JIANGNAN UNIV
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