Oral mucosa cell culture method

Abstract

The invention discloses an oral mucosa cell culture method. The oral mucosa cell culture method is characterized by comprising the steps: preparation work before culture, specific culture process andfinish work after culture, wherein preparation work before culture includes preparation of a culture instrument and preparation of oral mucosa cells. According to the oral mucosa cell culture method,a serum-free medium is utilized, the cells can realize stable passage for 4-5 generations, the multiplication capacity of the former three generations is high, a certain proportion of giant cells occur in the fourth generation, the cells of the fifth generation are aged, thus from the angle of tissue engineering, the research supports the use of the cells of the cultured third generation, the certain number of cells are met, it is ensured that the cells have the good multiplication capacity, the passage number of the mucosa epithelial cells is closely related to the number of inoculated cells,uncertain factors are few, the experimental efficiency is high, disinfection and sterilization treatment is adopted before and after an experiment, the uncertain factors of the experiment are furtherreduced, and thus the experimental efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of oral mucosal cell culture, in particular to a method for oral mucosal cell culture. Background technique [0002] The cells in the oral cavity have the same shape and function, and are collectively referred to as oral cells. Oral cell culture refers to simulating the physiological environment in the body. Under appropriate conditions, the living tissue cells can survive, grow and proliferate in vitro, and maintain their Structural function. [0003] The traditional oral mucosal cell culture method does not have systematic and standardized experimental procedures in practical application, and there are many uncertain factors in the experiment. It is necessary to make multiple sets of comparative experiments to obtain accurate experimental results, which greatly affects the experimental efficiency. For example, digestion Due to the influence of the preparation of the liquid and the main ions in the medium,...

AI Technical Summary

Problems solved by technology

[0003] The traditional oral mucosal cell culture method does not have systematic and standardized experimental procedures in practical application, and there are many uncertain factors in the experiment. It is necessary to make multiple sets of comparative experiments to obtain accurate experimental results, which greatly affects the experimental efficiency. For example, digestion Due to the influence of the preparation of the liquid and the main ions in the medium, it is impossible to quickly obtain high-purity OMECs with typical epithelial cell characteristics, so it is necessary to design a culture method for oral mucosal cells