Culture method for regulatory T cells (Tregs) of mice by inducing of aggregation-induced emission luminogen (AIEgen) DPA-SCP

A technology of DPA-SCP and aggregation-induced luminescence, which is applied in the field of immunology research to achieve the effects of strong feasibility, high induction efficiency and low cost
CN110423724AInactive Publication Date: 2019-11-08TIANJIN UNIV
3 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2019-11-08
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a culture method for regulatory T cells (Tregs) of mice by inducing of aggregation-induced emission luminogen (AIEgen) DPA-SCP. Multiple cell factors are used, AIEgen DPA-SCP isadded for illumination stimulation, and a large number of Tregs are induced from splenocytes of mice by induction amplification in vitro. The culture method is simple in induction process and high infeasibility; the culture method is short in cell culture period and low in cost; and the culture method is high in induction efficiency, good in repeatability and applicable to culture of Tregs. Compared with traditional methods, the culture method can more effectively induce a large number of CD4<+>CD25<+>Foxp3<+> Tregs, provides a convenient culture method for further study and has very good promotion values.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of immunology research, and relates to a method for culturing mouse regulatory T cells induced by an aggregation-inducing luminescent molecule DPA-SCP. Background technique

[0002] T cell (regulatory T cell, Treg) is CD4 + A subpopulation of T cells with important physiological roles in preventing the development of autoimmune diseases and maintaining self-tolerance. In 1995, Sakaguchi et al. obtained for the first time CD4 which has the function of maintaining autoimmune tolerance and immunosuppression + CD25 + Treg cells, this cell subset accounts for about 5% to 10% in peripheral blood of mice and humans. CD4 + CD25 + Treg cells mainly include innate Treg cells (nTreg) generated in the thymus, adaptive Treg cells (iTreg) induced by ordinary T cells, and endogenous induced Treg cells (pTreg) differentiated from antigen-stimulated T cells in peripheral tissues. , this type of cells plays an important role in m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More