Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bischofia polycarpa tissue culture and rapid propagation method

A technology for tissue culture, fast propagation and Chongyang tree, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of shortening the breeding cycle, low cultivation cost and simple operation.

Active Publication Date: 2019-11-12
ANHUI AGRICULTURAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After reviewing the literature, it is found that scholars' research on Chongyang tree mainly focuses on the cultivation technology of Chongyang tree and the technology of disease and insect pest control, but there is no report on the complete tissue culture and rapid propagation technology of Chongyang tree

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bischofia polycarpa tissue culture and rapid propagation method
  • Bischofia polycarpa tissue culture and rapid propagation method
  • Bischofia polycarpa tissue culture and rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of tissue culture rapid propagation method of Chongyang wood of the present embodiment, with the Chongyang wood axillary bud stem section as explant, comprises the following steps:

[0041] A1. Acquisition and disinfection of explants: The experimental materials come from excellent individual plants with strong flooding resistance in Xinguanzhou Forest Farm, Anqing City, Anhui Province; the freshly picked axillary bud stems of Chongyang wood were rinsed with washing powder for 1min and running water Rinse for 1 hour, then use 75% alcohol to rinse quickly for 30 seconds in an ultra-clean workbench, rinse with sterile water for 4 times, soak in 1% sodium hypochlorite for 5 minutes, and finally, rinse with sterile water for 6 times; Drain the moisture from the leaves.

[0042] A2. Induction culture: Cut the stem section of the axillary buds of Chongyang chrysanthemum after absorbing the water into 1cm under sterile conditions 2 Cut into pieces of different sizes, a...

Embodiment 2

[0046] A kind of tissue culture rapid propagation method of Chongyang wood of the present embodiment, with the Chongyang wood axillary bud stem section as explant, comprises the following steps:

[0047] A1. Acquisition and disinfection of explants: the experimental materials come from excellent individual plants with strong flooding resistance in Xinguanzhou Forest Farm, Anqing City, Anhui Province; the freshly picked axillary bud stems of Chongyang scallopedifolia were rinsed with washing powder for 0.5min, Rinse with running water for 0.5h, then use 75% alcohol to rinse quickly for 25s, rinse with sterile water for 3 times, soak in 1% sodium hypochlorite for 4min in the ultra-clean workbench, and finally, rinse with sterile water for 5 times; Absorbent paper absorbs the moisture on the leaves for later use.

[0048] A2. Induction culture: Cut the stem section of the axillary buds of Chongyang chrysanthemum after absorbing the water into 1cm under sterile conditions 2 Cut i...

Embodiment 3

[0052] A kind of tissue culture rapid propagation method of Chongyang wood of the present embodiment, with the Chongyang wood axillary bud stem section as explant, comprises the following steps:

[0053] A1. Acquisition and disinfection of explants: the experimental materials come from excellent individual plants with strong flooding resistance in Xinguanzhou Forest Farm, Anqing City, Anhui Province; freshly picked axillary bud stems of S. chrysalis Chongyang are rinsed with washing powder for 1.5min, Rinse with running water for 1.5 hours, then use 75% alcohol to rinse quickly for 35 seconds in an ultra-clean workbench, rinse with sterile water for 5 times, soak in 1% sodium hypochlorite for 6 minutes, and finally rinse with sterile water for 7 times; Absorbent paper absorbs the moisture on the leaves for later use.

[0054] A2. Induction culture: Cut the stem section of the axillary buds of Chongyang chrysanthemum after absorbing the water into 1cm under sterile conditions ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a bischofia polycarpa tissue culture and rapid propagation method, referring to tissue culture and propagation by taking the bischofia polycarpa stem with axillary buds or the bischofia polycarpa blade as the explant. When the bischofia polycarpa stem with axillary buds is taken as the explant, the culture method includes disinfecting the stem with axillary buds, culturing the stem with axillary buds in an inducing medium to obtain clustered seedlings, cutting the clustered seedlings into single seedling, inoculating the seedlings on a rooting medium to perform rooting culture, and finally, hardening and transplanting the seedlings which are rooted, so as to realize rapid propagation of bischofia polycarpa. When the bischofia polycarpa blade is taken as the explant,the culture method includes disinfecting seeds of bischofia polycarpa, performing culture of sterile blade, taking the sterile blade as the explant, performing induction of callus, callus differentiation and rooting culture sequentially, thereby building the bischofia polycarpa tissue culture and rapid propagation system. The method has the advantages of simple operation, low culture cost, high propagation efficiency, low influence from natural environment, good marketing and industrialized application prospects.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for tissue culture and rapid propagation of Chongyang wood. Background technique [0002] Bischofia polycarpa (scientific name: Bischofia polycarpa) is a deciduous tree of the genus Bischofia in the family Euphorbiaceae. It is a native tree species in my country and a famous garden ornamental tree species in China. Chongyang wood has the advantages of shade tolerance, loose soil requirements, water and humidity resistance, well-developed root system, and strong wind resistance. It is often distributed in the Qinling Mountains, south of the Huaihe River Basin, the middle and lower reaches of the Yangtze River, and some water network areas. Chongyang wood has a medium growth rate, luxuriant leaves, magnificent tree appearance, and red leaves in autumn. It is usually cultivated as a street tree and ornamental tree in gardens, and it is widely used in landscaping. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 项艳王雅梅王瑞佳何婷
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com