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A basophil counting set for a five-differentiation blood cell analyzer

A blood cell analyzer and basophil technology, applied in the field of cell detection, can solve problems such as broken red blood cells, achieve accurate detection results, stable performance, and reduce costs

Active Publication Date: 2021-11-09
江苏美诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the representative Mindray series of domestic five-category blood analyzers, the early basophils were detected by the impedance method, and later the instrument was upgraded. With the continuous application of a large number of new technologies on the instrument, it gradually developed to the optical method. detection, but because of the interference of broken red blood cells, platelets and fat droplets, etc., there is still a great influence

Method used

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  • A basophil counting set for a five-differentiation blood cell analyzer
  • A basophil counting set for a five-differentiation blood cell analyzer
  • A basophil counting set for a five-differentiation blood cell analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The hemolytic agent provided by the invention for counting leukocytes and basophils has a formula as follows:

[0025]

[0026] Mix the above-mentioned component ratios, add purified water to make it up to 1L, stir at room temperature until it dissolves completely, and filter with a 0.2 μm filter membrane, wherein the dyeing solution is 0.05% of polymethine dye and 99.9% of ethylene glycol. is the mass percent concentration. Tested on Sysmex XN-1000, the blank value meets the requirements.

Embodiment 2

[0028] The hemolytic agent provided by the invention for counting leukocytes and basophils has a formula as follows:

[0029]

[0030] Mix the above-mentioned component ratios, add purified water to make it up to 1L, stir at room temperature until it dissolves completely, and filter with a 0.2 μm filter membrane, wherein the dyeing solution is 0.05% of polymethine dye and 99.9% of ethylene glycol. is the mass percent concentration. Tested on Sysmex XN-1000, the blank value meets the requirements.

Embodiment 3

[0032] The hemolytic agent provided by the invention for counting leukocytes and basophils has a formula as follows:

[0033]

[0034] Mix the above-mentioned component ratios, add purified water to make it up to 1L, stir at room temperature until it dissolves completely, and filter with a 0.2 μm filter membrane, wherein the dyeing solution is 0.05% of polymethine dye and 99.9% of ethylene glycol. is the mass percent concentration. Tested on Sysmex XN-1000, the blank value meets the requirements.

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Abstract

The invention discloses a basophil counting set for a five-differentiation blood cell analyzer, which belongs to the technical field of cell detection. The invention stains white blood cells and basophils without staining red blood cells and fat droplets, eliminates adverse effects, and is more accurate than other existing technologies in measuring the counts of white blood cells and basophils in human whole blood. The hemolytic agent is a buffer solution containing cationic surfactant and nonionic surfactant, wherein the concentration of cationic surfactant is 0.1g / L~20g / L, and the concentration of nonionic surfactant is 0.5g / L~30g / L, the pH value of the buffer solution is 2.0-5.0; the dyeing solution is a solution containing polymethine dye and ethylene glycol, wherein the concentration of polymethine dye is 0.005%, and ethylene glycol is 99.9%. For the present invention, the components of the hemolytic agent are simplified and the staining solution is added. The reaction time is only 10-20s, and the determination of the differential count of basophils and white blood cells can be completed, which has a high degree of accuracy.

Description

technical field [0001] The invention relates to a basophil counting set for a five-differentiation blood cell analyzer, which belongs to the technical field of cell detection. Background technique [0002] Basophils are a type of granulocytes with the least proportion in the whole blood of normal people, accounting for 0-1% of the total number of white blood cells. Clinically, myeloproliferative diseases, heavy metal poisoning, chronic myelogenous leukemia, infection The number of basophils will change significantly in certain food allergies and other diseases, so the accurate count of basophils in human whole blood has direct guiding significance for the diagnosis of these diseases. [0003] Before the five-differential blood cell analyzer was launched, basophils were clinically stained and counted with chemical dyes, including toluidine blue staining, Wright-Giemsa composite staining, and neutral red staining. These methods are intuitive It is effective, but it takes a lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10G01N1/30
CPCG01N1/30G01N15/10G01N2001/302G01N2015/1006
Inventor 伍春宝陈勇
Owner 江苏美诚生物科技有限公司
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