Basophilic granulocyte counting suit for five-classification hematology analyzer

Abstract

The invention discloses a basophilic granulocyte counting suit for a five-classification hematology analyzer, and belongs to the technical field of cell detection. White blood cells and basophilic granulocytes are dyed; red blood cells and fat droplets cannot be dyed; and the adverse influence is avoided. Compared with the prior art, the basophilic granulocyte counting suit for the five-classification hematology analyzer is more accurate for testing the count of the white blood cells and the basophilic granulocytes in human whole blood. A hemolytic agent is a buffer solution containing cationic surface active agents and non-ionic surface active agents, wherein the concentration of the cationic surface active agents is 0.1g / L to 20g / L, the concentration of the non-ionic surface active agents is 0.5g / L to 30g / L, and the pH value of the buffer solution is 2.0 to 5.0. A dyeing solution is a solution containing polymethine dye and ethanediol, wherein the concentration of the polymethine eyeis 0.005 percent, and the concentration of the ethanediol is 99.9 percent. The basophilic granulocyte counting suit has the advantages that the ingredients of the hemolytic agent are simplified; thedyeing solution is added; the reaction time is only 10 to 20s, the testing of performing classified counting on the basophilic granulocyte and the white blood cells can be completed; and high accuracyis realized.

Description

technical field [0001] The invention relates to a basophil counting set for a five-differentiation blood cell analyzer, which belongs to the technical field of cell detection. Background technique [0002] Basophils are a type of granulocytes with the least proportion in the whole blood of normal people, accounting for 0-1% of the total number of white blood cells. Clinically, myeloproliferative diseases, heavy metal poisoning, chronic myelogenous leukemia, infection The number of basophils will change significantly in certain food allergies and other diseases, so the accurate count of basophils in human whole blood has direct guiding significance for the diagnosis of these diseases. [0003] Before the five-differential blood cell analyzer was launched, basophils were clinically stained and counted with chemical dyes, including toluidine blue staining, Wright-Giemsa composite staining, and neutral red staining. These methods are intuitive It is effective, but it takes a lo...

AI Technical Summary

Problems solved by technology

For example, the representative Mindray series of domestic five-category blood analyzers, the early basophils were detected by the impedance method, and later the instrument was upgraded. With the continuous application of a large number of new technologies on the instrument, it gradually developed to the optical method. detection, but because of the interference of broken red blood cells, platelets and fat droplets, etc., there is still a great influence

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