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Method for regulating the preparation of hydroxyapatite with excellent cytocompatibility

A hydroxyapatite and compatibility technology, applied in chemical instruments and methods, phosphorus compounds, inorganic chemistry, etc., can solve the problem that physicochemical properties and cell compatibility need to be improved, it is difficult to obtain uniform crystalline particles, and it is difficult to scale. Production and other problems, to achieve the effects of excellent cell compatibility and degradability, short preparation cycle, high phase purity and crystallinity

Active Publication Date: 2021-09-03
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many factors affecting the preparation of hydroxyapatite by the sol-gel method, and it is difficult to obtain particles with good crystallization, uniform particles, high purity and controllable shape and size.
The grains prepared by the hydrothermal method have high crystallinity and can be adjusted, but the required high temperature and high pressure conditions make it difficult to scale production
Moreover, the hydroxyapatite prepared by these existing technologies still has certain biological toxicity, which will cause additional side effects to the human body, and the physicochemical properties and cell compatibility need to be improved

Method used

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  • Method for regulating the preparation of hydroxyapatite with excellent cytocompatibility
  • Method for regulating the preparation of hydroxyapatite with excellent cytocompatibility
  • Method for regulating the preparation of hydroxyapatite with excellent cytocompatibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, the method for regulating and preparing hydroxyapatite with excellent cytocompatibility provided in Example 1 is:

[0026] Dissolve 3.5423g of calcium nitrate tetrahydrate and 4.5042g of glycine in 50ml of deionized water and stir for 10 minutes; dissolve 1.3206g of diammonium hydrogen phosphate in 50ml of deionized water and stir for 10 minutes; Slowly drop into the mixed solution of calcium nitrate tetrahydrate and glycine in stirring at a speed of 1 / min, keep the temperature at 25°C, adjust the pH=9 of the whole dropping process with ammonia water, finish the dropping within 10min, and Continue to stir for 20 min after dropping to make the solution system uniform. Pour the obtained precursor into a microwave reactor, set the wattage of the microwave digestion instrument to 300W, the temperature to 160°C, and the time to 40min. After the microwave hydrothermal reaction is over, pour it into a 50ml centrifuge tube, and perform centrifugal w...

Embodiment 2

[0028] Such as figure 1 As shown, the method for regulating and preparing hydroxyapatite with excellent cytocompatibility provided in Example 2 is:

[0029] Dissolve 3.5423g of calcium nitrate tetrahydrate and 9.0084g of glycine in 50ml of deionized water and stir for 10 minutes; dissolve 1.3206g of diammonium hydrogen phosphate in 50ml of deionized water and stir for 10 minutes; Slowly drop into the mixed solution of calcium nitrate tetrahydrate and glycine in stirring at a speed of 1 / min, keep the temperature at 25°C, adjust the pH of the whole dropping process with ammonia water to 9, finish the dropping within 10min, and Continue to stir for 20 min after dropping to make the solution system uniform. Pour the obtained precursor into a microwave reactor, set the wattage of the microwave digestion instrument to 300w, the temperature to 160°C, and the time to 40min. After the microwave hydrothermal reaction is over, pour it into a 50ml centrifuge tube, and perform centrifuga...

Embodiment 3

[0031] The method for regulating and preparing hydroxyapatite with excellent cytocompatibility provided in Example 3 is as follows:

[0032] Dissolve 3.5423g of calcium nitrate tetrahydrate and 6.3054g of serine in 50ml of deionized water, and stir for 10 minutes; dissolve 1.3206g of diammonium hydrogen phosphate in 50ml of deionized water, and stir for 10 minutes; Slowly drop into the mixed solution of calcium nitrate tetrahydrate and serine in stirring at a speed of 1 / min, keep the temperature at 25°C, adjust the pH of the whole dropping process with ammonia water to 9, finish the dropping within 10min, and Continue to stir for 20 min after dropping to make the solution system uniform. Pour the obtained precursor into a microwave reactor, set the wattage of the microwave digestion instrument to 300w, the temperature to 160°C, and the time to 40min. After the microwave hydrothermal reaction is over, pour it into a 50ml centrifuge tube, and perform centrifugal washing with de...

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Abstract

The invention provides a method for regulating and preparing hydroxyapatite with excellent cytocompatibility, which is characterized in that it comprises the following steps: Step 1. Prepare Ca(NO 3 ) 2 4H 2 O aqueous solution and (NH 4 ) 2 HPO 4 aqueous solution, and in Ca(NO 3 ) 2 4H 2 Add small molecule amino acids in the O aqueous solution, and the concentration ratio of amino acids and calcium ions is 4:1 or 8:1; Step 2. Set the parameters when dropping and mixing, and (NH 4 ) 2 HPO 4 The mixed aqueous solution of amino acid and amino acid was slowly added dropwise to the stirring Ca(NO 3 ) 2 4H 2 O aqueous solution; step 3. put the obtained precursor into a microwave digestion instrument, set the microwave hydrothermal parameters to react; step 4. carry out centrifugal washing to the solid obtained after the reaction, then freeze-dry to remove excess moisture, and obtain Hydroxyapatite.

Description

technical field [0001] The invention belongs to the technical field of hydroxyapatite preparation, and in particular relates to a method for regulating and preparing hydroxyapatite with excellent cell compatibility. [0002] technical background [0003] Hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 ), as a main mineral widely present in animal bones and teeth, is currently the material of choice for biomedical applications. Hydroxyapatite has good physical and chemical properties, but these properties are affected by the surface morphology, size, crystallinity, etc. of the crystal itself. These factors lead to differences in surface properties, mechanical properties, and biological properties of hydroxyapatite, which lead to differences in applications. [0004] Traditional synthesis methods include: precipitation method, hydrothermal method, sol-gel method and so on. Among them, the chemical precipitation method has a simple process, but the disadvantage is that the crystal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B25/32B82Y40/00
CPCC01B25/325C01P2002/72C01P2004/04C01P2004/62C01P2004/64
Inventor 王友法陈甲
Owner WUHAN UNIV OF TECH
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