37 results about "Ca2 concentration" patented technology

Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.

A protein body derivable from Fabaceae has a reversible, anisotropic contractability such that the protein body becomes thicker perpendicular to a longitudinal axis of the protein body and shorter along the longitudinal axis of the protein body when increasing a calcium ion concentration in a medium surrounding the protein body past a threshold value of 30 nM. The protein body becomes thinner perpendicular to the longitudinal axis and longer along the longitudinal axis when decreasing the calcium ion concentration below the threshold value of 30 nM. Also, the protein body becomes thicker in the direction perpendicular to the longitudinal axis when increasing a pH value of a medium surrounding the protein body to a value above 9.5 without becoming shorter along the longitudinal axis. The protein body becomes thinner in the direction perpendicular to the longitudinal axis without becoming longer decreasing the pH value below 9.5.

The invention discloses an acid pressure simulation method considering the dynamic process of mucovisic acid. The relationship between the viscosity of mucovisic acid and shear rate, temperature, calcium ion concentration, pH value and surfactant concentration is respectively measured, and a mucoviscic constitutive equation is established. ; Simulate the flow and reaction process of viscous acid in the fracture system, and obtain the pressure profile in the fracture system; take the pressure profile in the fracture system as the inner boundary condition, and the ground pressure as the outer boundary condition, simulate the calculation of the variation of viscous acid in the matrix the reaction process; simulate the viscosity change of the acid solution in the matrix system, and calculate the fluid loss velocity; bring the obtained fluid loss velocity into the pressure profile of the fracture system in the step. The invention is used to solve the problem that the acid pressure simulation technology in the prior art does not consider the dynamic filtration process of mucoacid, resulting in a low coincidence rate between the simulated seam length of the mucoacid acid pressure and the actual situation, and realizes that the simulation can be obtained considering the viscous acid. The purpose of acid fracturing fracture morphology after acid dynamic drop rate.

In the broadest aspect, the invention provides a composition for and a method of prophylactic and / or therapeutic treatment of a animal / mammal for any viral disease, mixed bacterial and viral infections, bacterial infections, bacterial endotoxins, bacterial exotoxins, autoimmune diseases, and cellular or humoral mediated allergic conditions that is caused by any virus that relies on the maintenance of specific calcium ion concentrations for the post ribosomal RNA synthesis processing of viral protein translation, transportation and processing of viral structural components by utilizing a therapeutic amount of a blocker selected from the group consisting of a calcium channel blocker, a metabolite thereof, a calmodulin blocker and a metabolite thereof, and a pharmaceutical acceptable diluent or carrier; and / or is caused by damage to the animal / mammal by a pathological immune response to antigens.

The invention relates to a method for representing plasma induced intracellular absolute calcium ion concentration. According to the method, a prepared cell suspension is added into an optimized fluo-3AM, 0.8 mM Pluronic F-127 is added, and a maximum fluorescence intensity Fmax value is obtained, or a calcium ion chelating agent EGTA is added, and a minimum fluorescence intensity Fmin value is obtained; the prepared cell suspension is added into the optimized fluo-3AM and then subjected to atmospheric pressure cold plasma treatment, and by combining with a multifunctional microplate reader todetermine an F value, the absolute calcium ion concentration is calculated according to a formula. The method for measuring the absolute calcium ion concentration of cells by combining the fluorescentprobe flou-3AM with the multifunctional microplate reader is achieved and has the advantages of being sensitive, highly efficient, simple and accurate in determination and representing the intracellular absolute calcium ion concentration.

The present disclosure relates to the generation of an activated platelet product in which one or more of the presence or absence of clots, the timing of clot formation (if present), and / or the mechanical strength of clots (if present) is controlled by the presence or concentration of calcium ions during the activation process. In certain embodiments, the calcium ion concentration is controlled in the presence of pulsed electric fields or a chemical activator (e.g., thrombin) as part of the activation process.

The invention discloses an antibiotic-free functional concentrated feed for lactating sows. The feed mainly includes fermented soybean meal, imported fish meal, alfalfa granules, valine, Bacillus subtilis, Eucommia extract, yeast cell wall, calcium formate, NCG, coconut oil, phytase, NSP enzyme, glucose oxidase, organic trace elements . The concentrated feed quickly replenishes energy during the delivery stage of sows, increases the concentration of calcium ions in the body, shortens the delivery process of sows, reduces the number of stillbirths, and accelerates the discharge of lochia. By selecting high-quality feed raw materials, while improving the immunity of sows to achieve resistance-free, the lactation performance of sows can be improved, and the weight gain of weaned piglets can be increased.

The invention provides a non-invasive detection method for quickly and effectively evaluating the outcome of in vitro fertilization, which comprises the following steps: blending the obtained sperm into the culture fluid; dripping the prepared pre-fertilization culture fluid into the culture dish, Prepare a plurality of pre-fertilization culture drops in a petri dish; inject the single ovum taken out into each pre-fertilization culture drop respectively, and cultivate for 4-5 hours; absorb part of the fertilization drop in the fertilization drop, centrifuge, and take The supernatant was diluted; the diluted fertilized drops were detected with an automatic biochemical analyzer for calcium ion concentration; the difference in calcium ion concentration in the fertilized drops was calculated to predict the difference in OPN outcome and / or 2PN outcome. In this study, the fertilization drops (Day 0) that were exchanged for the first 4-5 hours were collected for early detection. The results of the study can directly predict the fertilization outcome of the 2PN stage (Day 1), which will help improve the early clinical judgment of the fertilization outcome and provide a basis for further diagnosis and treatment. Provide guidance.