36 results about "Ca2 concentration" patented technology

Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.

The invention discloses a composition for treating diarrhea and a preparation thereof, relating to a medicinal composition for stopping the diarrhea. The invention also relates to a preparation of the medicinal composition for stopping the diarrhea. The medicinal composition is mainly prepared from the main raw materials of 1 part of Racecadotril and 0.25-25 parts of berberine hydrochloride as well as 0.01-250 parts of auxiliary materials in proportion. The medicinal composition has scientific and reasonable prescription, and can be used for inhibiting the rise of calcium ion concentration in colon smooth muscle cells and improving the motion functional coordination of the intestinal tract to realize the effect on stopping the diarrhea mainly by inhibiting the activity of enkephalinase and further inhibiting the excessive secretion of water and electrolyte via the Racecadotril, and simultaneously by the stronger bactericidal effect of the berberine hydrochloride on escherichia coli, Streptococcus hemolyticus, Diplococcus pneumoniae and the like.

The present invention relates to a preventive or a therapeutic agent for hepatopathy, comprising, as an effective ingredient, a polypeptide derivative having an activity of binding to growth hormone secretagogue receptor and thus elevating intracellular calcium ion concentration, and having one amino acid sequence selected from the group consisting of amino acid sequences represented by SEQ ID NOS: 1 to 22, or an amino acid sequence in which one to several amino acids are deleted, substituted or added at the amino acid residues other that the first to forth amino acid residues from the amino-terminus in the said amino acid sequence, or a pharmacologically acceptable salt thereof. The preventive or the therapeutic agent for hepatopathy in accordance with the present invention is useful as a drug suitable for treating or preventing hepatic diseases such as hepatitis, liver cirrhosis and hepatic insufficiency. Further, the preventive or the therapeutic agent for hepatopathy in accordance with the present invention is also useful as an agent of promoting hepatic regeneration and hepatic function recovery after hepatectomy.

The invention relates to a calcium ion selective membrane and a preparation method thereof, and belongs to the technical field of blood gas biochemical sensors. The calcium ion selective membrane is used for detecting the concentration of calcium ions in body fluid. The calcium ion selective membrane comprises a macromolecule binding agent, an ion carrier, a carrier solvent, an anion interference agent and a surface active agent. The macromolecule binding agent, the ion carrier, the carrier solvent, the anion interference agent and the surface active agent are dissolved in an ethyl acetate solution, and coating and drying are performed to obtain the calcium ion selective membrane. The prepared calcium ion selective membrane overcomes the defects that the carrier solvent of a traditional ion selective membrane leaks and the strength of the membrane is not high. The prepared calcium ion selective membrane is high in strength, good in tenacity, high in measurement precision, good in long-term stability and low in lower detection limit, the preparation method is simple, the production period is short, the production cost is low, and the calcium ion selective membrane is suitable for large-scale industrial production.

The invention discloses an acid fracturing simulation method considering a dynamic process of variable viscosity acid. The method comprises the following steps of: respectively measuring the relationship between the viscosity of the variable-viscosity acid and a shearing rate, temperature, calcium ion concentration, a pH value and surfactant concentration and establishing a variable-viscosity constitutive equation, simulating the flowing and reaction process of the variable-viscosity acid liquid in a fracture system to obtain a pressure profile in the fracture system, simulating and calculating the reaction process of the variable-viscosity acid in a matrix by taking the pressure profile in the fracture system as an inner boundary condition and the ground pressure as an outer boundary condition, simulating the viscosity change condition of the acid liquid in the matrix system and calculating to obtain the filtration speed, and bringing the obtained filtration speed into the pressure profile in the fracture system in the step. The method is used for solving the problem that in the prior art, the acid fracturing simulation technology does not consider the dynamic filtration process of the variable-viscosity acid, so that the coincidence rate of the variable-viscosity acid fracturing simulation fracture length and the actual situation is not high, and the purpose that the acid fracturing fracture form considering the dynamic reduction rate of the variable-viscosity acid can be obtained through simulation is achieved.

The present claimed invention provides a calcium ion concentration measuring device that can measure a concentration of a calcium ion in blood of a domestic animal with high accuracy without being affected by temperature on the spot where the blood is collected. The calcium ion concentration measuring device is a portable one for measuring the concentration of the calcium ion in the blood of the domestic animal, and comprises a responsive membrane that selectively reacts with the calcium ion, an internal liquid stored in a space separated from the outside by the responsive membrane, and an internal electrode arranged to be in contact with the internal liquid, and the isothermal intersection point of the internal liquid is set within a range of the concentration of the calcium ion contained in the blood of the domestic animal.

Disclosed is a complex of the 11 kDa Ca2+ binding protein of 11 and Hepatitis B virus polymerase (HBVPol) which moves to Promyelocytic Leukemia Nuclear Body (PMLNB). Furthermore, the present invention discloses a method for controlling movement of the HBVPol / p11 complex into the nucleus of HepG2 cell with adjusting of intracellular Ca2+ ion concentration by administrating an agent for controlling calcium ion concentration in HepG2 cells.

A protein body derivable from Fabaceae has a reversible, anisotropic contractability such that the protein body becomes thicker perpendicular to a longitudinal axis of the protein body and shorter along the longitudinal axis of the protein body when increasing a calcium ion concentration in a medium surrounding the protein body past a threshold value of 30 nM. The protein body becomes thinner perpendicular to the longitudinal axis and longer along the longitudinal axis when decreasing the calcium ion concentration below the threshold value of 30 nM. Also, the protein body becomes thicker in the direction perpendicular to the longitudinal axis when increasing a pH value of a medium surrounding the protein body to a value above 9.5 without becoming shorter along the longitudinal axis. The protein body becomes thinner in the direction perpendicular to the longitudinal axis without becoming longer decreasing the pH value below 9.5.

The invention discloses an acid pressure simulation method considering the dynamic process of mucovisic acid. The relationship between the viscosity of mucovisic acid and shear rate, temperature, calcium ion concentration, pH value and surfactant concentration is respectively measured, and a mucoviscic constitutive equation is established. ; Simulate the flow and reaction process of viscous acid in the fracture system, and obtain the pressure profile in the fracture system; take the pressure profile in the fracture system as the inner boundary condition, and the ground pressure as the outer boundary condition, simulate the calculation of the variation of viscous acid in the matrix the reaction process; simulate the viscosity change of the acid solution in the matrix system, and calculate the fluid loss velocity; bring the obtained fluid loss velocity into the pressure profile of the fracture system in the step. The invention is used to solve the problem that the acid pressure simulation technology in the prior art does not consider the dynamic filtration process of mucoacid, resulting in a low coincidence rate between the simulated seam length of the mucoacid acid pressure and the actual situation, and realizes that the simulation can be obtained considering the viscous acid. The purpose of acid fracturing fracture morphology after acid dynamic drop rate.

The invention provides a method for regulating and preparing hydroxyapatite with excellent cytocompatibility, which is characterized in that it comprises the following steps: Step 1. Prepare Ca(NO 3 ) 2 4H 2 O aqueous solution and (NH 4 ) 2 HPO 4 aqueous solution, and in Ca(NO 3 ) 2 4H 2 Add small molecule amino acids in the O aqueous solution, and the concentration ratio of amino acids and calcium ions is 4:1 or 8:1; Step 2. Set the parameters when dropping and mixing, and (NH 4 ) 2 HPO 4 The mixed aqueous solution of amino acid and amino acid was slowly added dropwise to the stirring Ca(NO 3 ) 2 4H 2 O aqueous solution; step 3. put the obtained precursor into a microwave digestion instrument, set the microwave hydrothermal parameters to react; step 4. carry out centrifugal washing to the solid obtained after the reaction, then freeze-dry to remove excess moisture, and obtain Hydroxyapatite.

In the broadest aspect, the invention provides a composition for and a method of prophylactic and / or therapeutic treatment of a animal / mammal for any viral disease, mixed bacterial and viral infections, bacterial infections, bacterial endotoxins, bacterial exotoxins, autoimmune diseases, and cellular or humoral mediated allergic conditions that is caused by any virus that relies on the maintenance of specific calcium ion concentrations for the post ribosomal RNA synthesis processing of viral protein translation, transportation and processing of viral structural components by utilizing a therapeutic amount of a blocker selected from the group consisting of a calcium channel blocker, a metabolite thereof, a calmodulin blocker and a metabolite thereof, and a pharmaceutical acceptable diluent or carrier; and / or is caused by damage to the animal / mammal by a pathological immune response to antigens.

The invention relates to a method for representing plasma induced intracellular absolute calcium ion concentration. According to the method, a prepared cell suspension is added into an optimized fluo-3AM, 0.8 mM Pluronic F-127 is added, and a maximum fluorescence intensity Fmax value is obtained, or a calcium ion chelating agent EGTA is added, and a minimum fluorescence intensity Fmin value is obtained; the prepared cell suspension is added into the optimized fluo-3AM and then subjected to atmospheric pressure cold plasma treatment, and by combining with a multifunctional microplate reader todetermine an F value, the absolute calcium ion concentration is calculated according to a formula. The method for measuring the absolute calcium ion concentration of cells by combining the fluorescentprobe flou-3AM with the multifunctional microplate reader is achieved and has the advantages of being sensitive, highly efficient, simple and accurate in determination and representing the intracellular absolute calcium ion concentration.

The present disclosure relates to the generation of an activated platelet product in which one or more of the presence or absence of clots, the timing of clot formation (if present), and / or the mechanical strength of clots (if present) is controlled by the presence or concentration of calcium ions during the activation process. In certain embodiments, the calcium ion concentration is controlled in the presence of pulsed electric fields or a chemical activator (e.g., thrombin) as part of the activation process.

The present invention is based on two important experimental observations: The first observation is that increased extracellular concentrations of ionized calcium are found in erosive arthritis and stimulate monocytic IL-1β release via the CaSR and GPRC6A. Simultaneous stimulation of monocytes with calcium ions and selected TLR ligands results in a 20-fold increased IL1β response compared to lipopolysaccharide (LPS) alone. During the crosstalk between GPCR and TLR signaling, phospholipase C is activated, which triggers calcium dependent potassium channels, resulting in potassium efflux, caspase-1 activation and IL-1β release. The amplification of IL1β secretion at sites of locally increased calcium ion concentrations aggravates rheumatoid arthritis. The second important observation is that both CaSR and GPRC6A, are highly expressed in the synovial membrane of patients with rheumatoid arthritis, but expression of GPRC6A, but not of CaSR, is lower in patients with osteoarthritis (s. FIG. 1). The latter is generally not accompanied by inflammation. Thus, expression of GPRC6A appears to be upregulated in chronic inflammatory situations. Based on these experimental observations the invention provides a method and compositions for the identification of agents that have a potential effect against chronic inflammatory conditions, in particular erosive arthritis and atherosclerosis.

The invention discloses an antibiotic-free functional concentrated feed for lactating sows. The feed mainly includes fermented soybean meal, imported fish meal, alfalfa granules, valine, Bacillus subtilis, Eucommia extract, yeast cell wall, calcium formate, NCG, coconut oil, phytase, NSP enzyme, glucose oxidase, organic trace elements . The concentrated feed quickly replenishes energy during the delivery stage of sows, increases the concentration of calcium ions in the body, shortens the delivery process of sows, reduces the number of stillbirths, and accelerates the discharge of lochia. By selecting high-quality feed raw materials, while improving the immunity of sows to achieve resistance-free, the lactation performance of sows can be improved, and the weight gain of weaned piglets can be increased.

The present disclosure relates to a Mitochondria-Associated endoplasmic reticulum Membrane (MAM)-specific fluorescence calcium sensor and the use thereof. The present disclosure can surmount the limitations of a conventional technique in that verification of calcium migration through MAM requires separate measurements of calcium ion concentrations within ER and mitochondria and situational explanations of the phenomena, and can directly measure concentrations in the paths through which calcium ions move to exclude influences on calcium ion changes through numerous different calcium ion channels existing in mitochondria, thereby providing a convenient and accurate MAM-specific calcium ion sensor.

The invention provides a non-invasive detection method for quickly and effectively evaluating the outcome of in vitro fertilization, which comprises the following steps: blending the obtained sperm into the culture fluid; dripping the prepared pre-fertilization culture fluid into the culture dish, Prepare a plurality of pre-fertilization culture drops in a petri dish; inject the single ovum taken out into each pre-fertilization culture drop respectively, and cultivate for 4-5 hours; absorb part of the fertilization drop in the fertilization drop, centrifuge, and take The supernatant was diluted; the diluted fertilized drops were detected with an automatic biochemical analyzer for calcium ion concentration; the difference in calcium ion concentration in the fertilized drops was calculated to predict the difference in OPN outcome and / or 2PN outcome. In this study, the fertilization drops (Day 0) that were exchanged for the first 4-5 hours were collected for early detection. The results of the study can directly predict the fertilization outcome of the 2PN stage (Day 1), which will help improve the early clinical judgment of the fertilization outcome and provide a basis for further diagnosis and treatment. Provide guidance.