Flowering day lily extract, preparation method and usage of flowering day lily extract, and antidepressant drug combination

A technology of day lily and extract, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of cumbersome process, undisclosed use of day lily, high extraction cost, etc., and achieve simple steps, excellent antidepressant effect and good application foreground effect

Pending Publication Date: 2019-12-03
绿之韵生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent shows that day lily has an antidepressant effect by detecting changes in neurotransmitter factors in mice. It does not disclose what kind of day lily is used, and it is extracted by heating and refluxing with 60%-90% ethanol. Then extract with water to obtain the antidepressant total flavonoids of day lily, the process is more cumbersome and the extraction cost is higher

Method used

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  • Flowering day lily extract, preparation method and usage of flowering day lily extract, and antidepressant drug combination
  • Flowering day lily extract, preparation method and usage of flowering day lily extract, and antidepressant drug combination
  • Flowering day lily extract, preparation method and usage of flowering day lily extract, and antidepressant drug combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 prepares flowering day lily water extract

[0049] (1) Grind the flowering daylily, add 8 times the mass of normal temperature water to extract for 6 hours, and concentrate the extract after extraction to obtain the first extract;

[0050] (2) Continue to add 8 times the mass of normal temperature water to the residue for extraction for 3 hours, and concentrate the extract after extraction to obtain the second extract;

[0051] (3) Combine the first extract and the second extract to obtain the third extract, and freeze the third extract at -80°C for 24 hours;

[0052] (4) Take the frozen third extract after freeze-drying for 72 hours, grind it into powder, and obtain the water extract of flowering day lily.

Embodiment 2

[0053] Example 2 Preparation of flowering daylily alcohol extract

[0054] (1) Grind the flowering daylily, add 8 times the mass of ethanol with a volume concentration of 85% to extract for 6 hours, and concentrate the extract after the extraction to obtain the first extract;

[0055] (2) Continue adding 8 times the mass of ethanol with a volume concentration of 85% to the residue for extraction for 3 hours, and concentrate the extract after extraction to obtain the second extract;

[0056] (3) Combine the first extract and the second extract to obtain the third extract, and freeze the third extract at -80°C for 24 hours;

[0057] (4) Freeze-dry the frozen third extract for 72 hours, grind and pulverize to obtain the alcohol extract of flowering day lily.

[0058] Figure 4 In order to prepare the total ion chromatograms of the water extract of flowering day lily and the alcohol extract of flowering day lily, it can be seen from the figure that there is a certain difference ...

Embodiment 3

[0059] Example 3 Effect of day lily extract on chronic unpredictable stress-induced depression in mice

[0060] (1) Establishment of mouse depression model induced by chronic unpredictable stress

[0061] 140 male ICR mice, weighing 18.0-22.0g, except for 10 normal control mice, the rest of the mice were subjected to chronic unpredictable stress, including fasting (12h), water deprivation (12h), forced swimming (10min) , strobe (12h), noise (30min), bondage (12h) (placed in a 50ml centrifuge tube, the diameter of the tube is 3.0cm, the length is about 10cm, and there are 6-7 ventilation holes with a diameter of 0.5mm on the tube wall), tilting Cage (12h), wet cage (12h), day and night reversed, etc., animals fasting and water deprivation during restraint.

[0062] Table 1 Chronic Unpredictable Stimulus Schedule

[0063]

[0064]

[0065] (2) Grouping and administration

[0066] After 28 days of continuous modeling, the animals were randomly divided into groups accordi...

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Abstract

The invention belongs to the technical field of natural medicine, and particularly relates to a flowering day lily extract, a preparation method and usage of the flowering day lily extract, and an antidepressant drug combination. The invention discloses the simple, economic and environment protective preparation method of the flowering day lily extract, and products of the preparation method, andfurther discloses application of the flowering day lily extract to preparation of antidepressant drug, and the antidepressant drug combination taking the flowering day lily extract as an active substance. The usage is verified through pharmacology experiment. According to the flowering day lily extract, the preparation method and usage of the flowering day lily extract, and the antidepressant drugcombination, application value of the flowering day lily is greatly developed, an intestinal microbial metabolites hypothesis is firstly adopted and applied to the flowering day lily extract, it is indicated that the flowering day lily extract can obviously influence intestinal microbe of a mice for treating depression, and good application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to flowering daylily extracts, preparation methods, uses and antidepressant pharmaceutical compositions. Background technique [0002] Day lily is also known as "day lily", "forgetfulness grass", and "hemerocallis". It is a plant of the genus Hemerocallis in the family Liliaceae and has rich nutritional ingredients. The edible part of the day lily is the flower bud. Once the day lily blooms and then eaten, it will produce a great sense of fiber, seriously affecting the taste of food, and it will usually be treated as waste. Day lily is a very characteristic traditional vegetable in our country. It is low in calories but has great nutritional value. Daylily is rich in nutrients, including carbohydrates, proteins, lipids, vitamins and minerals. Traditional Chinese medicine believes that the functions of daylily include: eliminating inflammation, resisting bact...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61P25/24
CPCA61K36/896A61P25/24A61K2236/331A61K2236/333A61K2236/39A61K2236/51
Inventor 曾建国胡国安卿志星劳嘉刘京宏贺炜
Owner 绿之韵生物工程集团有限公司
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