Phalaris arundinacea lactic acid bacteria for broussonetia papyrifera silage and application thereof

Abstract

The invention discloses phalaris arundinacea lactic acid bacteria for broussonetia papyrifera silage and an application thereof. According to the invention, a phalaris arundinacea silage sample is accurately weighed on an ultra-clean workbench, the weighed phalaris arundinacea silage sample is put into a triangular flask with normal saline to be sealed and oscillated, the lactic acid bacteria arefully dispersed and left to stand, and a 10 <-1> diluent is obtained; the 10 <-1> of diluents is sucked, is added into a centrifuge tube filled with sterilized normal saline, and fully and uniformly mixing to obtain the 10 <-2> diluent; continuously diluting is carried out by using the same method to prepare 10 <-4>, 10 <-5>... 10 <-N> series diluted bacterium solutions; after a culture dish is sterilized, a sterile MRS culture medium is added, then the solution is sucked from 10 <-1>, 10 <-3> and 10 <-5> diluent tubes and dripped on the surface of the culture medium, the culture dish is takenout after being cultured in a constant-temperature incubator, and is continuously purified with the MRS culture medium for five times, and the extracted lactic acid bacteria strain is obtained. Compared with the prior art, the strain extracted from the phalaris arundinacea is strong in viability and fast in acid production, belongs to lactobacillus plantarum, can well preserve the silage after being added into a broussonetia papyrifera material, and has popularization and application values.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a lactic acid bacterium for mulberry silage and its application. Background technique [0002] With the development of animal husbandry, the lack of feed is the main restrictive factor for the development of animal husbandry in China. The development of new forage resources is the focus of responding to the growing demand for animal products in China. Broussonetia papyrifera L. is a deciduous tree or shrub of the family Moraceae, native to mainland Southeast Asia and East Asia, including Taiwan, China, and widely distributed in China (about 300,000 hectares). It has the following characteristics: medicinal plants, antioxidant and anticancer activity, adaptation or resistance to traffic stress, metal adsorbents, fast-growing tree with lush foliage, high adaptability and tolerance. In addition, it has high nutritional value, rich in protein (18-24%) and flavonoids, and the ...

AI Technical Summary

Problems solved by technology

However, in the silage process of the mulberry tree, the silage effect is not good due to the extremely small number of lactic acid bacteria attached to the raw material

[0004] In order to solve the problem of silage failure due to the lack of lactic acid bacteria in mulberry raw materials, it is necessary to add additional lactic acid bacteria. However, the addition of conventional commercial lactic acid bacteria cannot improve the silage effect very well. Among the mulberry silage materials, there are relatively few studies on mulberry silage at present, and there are no reports on lactic acid bacteria additives specially used for mulberry silage.

Due to the extremely low content of lactic acid bacteria attached to its raw materials, the silage effect is not good

The commercial lactic acid bacteria currently on the market can not improve the silage effect of the mulberry tree very well. Therefore, it is necessary to extract lactic acid bacteria from other plant materials and add them to the silage material of the mulberry tree to improve the silage effect of the mulberry tree.

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