Electrochemical biosensor with rapid detection function

Abstract

The invention discloses an electrochemical biosensor with a rapid detection function. An electrode layer is provided with permeation and diffusion layers; the permeation and diffusion layers are covered with at least one kind of enzyme liquid; the enzyme liquids are directly dropwise added or adsorbed to the surfaces of the permeation and diffusion layers; the permeation and diffusion layers are cut into proper sizes, and the cut permeation and diffusion layers are fixed on the surfaces of electrodes; a modification liquid does not need to be prepared; the problem that the thickness of the modification liquid blocks the surfaces of the electrodes from detecting the reaction of the enzyme liquids and the problem that the enzymes are wrapped by the modification liquid are avoided; and the permeation and diffusion layers can filter blood cells, and the influence of the blood cells on a detection result is avoided.

Description

technical field [0001] The invention relates to the field of electrochemistry, more specifically, it relates to a rapid detection electrochemical biosensor. Background technique [0002] The following background art is provided to help the reader understand the present invention and is not to be admitted as prior art. [0003] The main purpose of biosensor research is to develop biosensors with practical application capabilities and their corresponding preparation techniques. The application of the screen printing technology makes it possible to prepare disposable electrochemical biosensor electrodes in batches. The method is simple in process and low in cost, and is suitable for industrial production of electrodes. Therefore, screen-printed electrodes based on the principle of electrochemical biosensors are widely used in the field of point-of-care detection (POCT). Physiological fluids such as blood, urine, sweat, and saliva contain many detection parameters that clinica...

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Problems solved by technology

[0004] At present, the detection principle of the electrode is mostly to modify the enzyme corresponding to the substrate on the working electrode, and measure the electrical signal through the enzymatic reaction. Therefore, the glucose sensor generally uses the enzymatic method, but the conditions required for the enzyme solution are relatively harsh. , and when the enzyme solution is fixed, the modified solution with a good concentration should be added dropwise on the electrode first. The modified solution has the function of diffusing the enzyme solution. The thickness of the modification solution in the middle cannot prevent the reaction of the enzyme solution on the electrode surface; or directly configure the diffusion modification solution and the enzyme solution together, and drop them on the electrode. During this process, the enzyme is likely to be wrapped up, and the detection signal will be relatively low. weak

Moreover, the selection and configuration of the modification solution is also the key. If the choice is not good, it will easily affect the reaction and detection results of the enzyme solution, which will greatly increase the research and development cost of the electrode test piece.

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