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Ratio-type fluorescent paper chip based on polyester fiber film, preparation method and application

A polyester fiber film, paper chip technology, applied in fluorescence/phosphorescence, material analysis by optical means, material excitation analysis, etc., can solve the problems of sophisticated and expensive equipment, complicated preprocessing, long detection time, etc., and achieve detection results. Clear and accurate, simple template elution, short detection time

Active Publication Date: 2022-05-13
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because it is difficult to degrade and easily remains in fruits and vegetables, it may directly or indirectly enter the human body through the food chain and pose a threat to human health, so the detection of 2,4-D in fruits and vegetables is of great significance
[0003] The commonly used 2,4-D detection technology is high-performance liquid chromatography, but this method is difficult to popularize due to the precision and expensive equipment, long detection time, and complicated pretreatment, which seriously limits the detection application of 2,4-D. From the above, it can be seen that 2,4-D is very likely to remain in fruits and vegetables, and will cause harm to the human body. Therefore, it is very important to establish a 2,4-D detection method that is efficient, convenient, portable, low-cost and environmentally friendly. Necessary

Method used

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  • Ratio-type fluorescent paper chip based on polyester fiber film, preparation method and application
  • Ratio-type fluorescent paper chip based on polyester fiber film, preparation method and application
  • Ratio-type fluorescent paper chip based on polyester fiber film, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of a ratio-type fluorescent paper chip based on a polyester fiber film, such as figure 1 shown, including the following steps:

[0032] S1: Grafting of CdTe QDs onto paper

[0033] Prepare the solution first, prepare 100mL of 1mM MES buffer solution, and make its pH 5.2, use MES buffer solution as solvent to prepare 20mL of EDC solution of 20mg / mL and 20mL of NHS solution of 10mg / L respectively, and use cut paper Cut the polyester fiber film paper, model DL42, into 1cm×1cm size with a knife, acidify with 20mL 0.2mol / L HCl, shake for 20min, pour off the HCl, and rinse with ultrapure water to remove the residual HCl solution 15mL CdTe QDs solution was mixed with 6mL EDC and shaken for 10min, poured into a petri dish with acidified fiber paper, added 6mL NHS, left for 1h and shaken for 16h, rinsed with ultrapure water three times to remove excess quantum dots. During this process, care should be taken to avoid light to prevent fluorescence quenching....

Embodiment 2

[0039] A preparation method of a ratio-type fluorescent paper chip based on a polyester fiber film, comprising the following steps:

[0040] S1: Grafting of CdTe QDs onto paper

[0041] Prepare the solution first, prepare 100mL of 1mM MES buffer solution, and make its pH 5.2, use MES buffer solution as solvent to prepare 20mL of EDC solution of 20mg / mL and 20mL of NHS solution of 10mg / L respectively, and use cut paper Cut the polyester fiber membrane paper, model DL42, into 1cm×1cm size, acidify with 20mL 0.2mol / L HCl, shake for 15min, pour off HCl, rinse with ultrapure water to remove residual HCl solution 15mL CdTe QDs solution was mixed with 6mL EDC and shaken for 10min, poured into a petri dish with acidified fiber paper, added 6mL NHS, left for 1h and shaken for 15h, rinsed with ultrapure water three times to remove excess quantum dots. During this process, care should be taken to avoid light to prevent fluorescence quenching.

[0042] S2: Preparation of MIPs

[0043]A...

Embodiment 3

[0047] A preparation method of a ratio-type fluorescent paper chip based on a polyester fiber film, comprising the following steps:

[0048] S1: Grafting of CdTe QDs onto paper

[0049] Prepare the solution first, prepare 100mL of 1mM MES buffer solution, and make its pH 5.2, use MES buffer solution as solvent to prepare 20mL of EDC solution of 20mg / mL and 20mL of NHS solution of 10mg / L respectively, and use cut paper Cut the polyester fiber film paper, model DL42, into 1cm×1cm size with a knife, acidify with 20mL 0.2mol / L HCl, shake for 25min, pour off the HCl, and rinse with ultrapure water to remove the residual HCl solution , Take 9mL CdTe QDs solution and 6mL EDC, mix and shake for 10min, pour it into a petri dish with acidified fiber paper, add 6mL NHS, let it stand for 1h, shake for 17h, rinse with ultrapure water three times to remove excess quantum dots. During this process, care should be taken to avoid light to prevent fluorescence quenching.

[0050] S2: Preparat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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PUM

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Abstract

The invention discloses a ratio-type fluorescent paper chip based on a polyester fiber membrane, a preparation method and an application thereof, and belongs to the technical field of material science engineering and microfluidic chips, and grafts CdTe QDs and RhB onto the surface of a polyester fiber membrane in sequence , and then adopt surface molecular imprinting technology to form 2,4-D imprinted holes on the surface of the polyester fiber membrane to obtain fluorescent molecularly imprinted paper; the fluorescent molecularly imprinted paper is installed on the carrier to obtain a ratio-type fluorescent paper chip; the present invention uses Compared with a single fluorescent paper chip, the two kinds of fluorescence can realize internal self-correction, making the detection results clearer and more accurate; it has the advantage of simple pre-treatment, and the fruits and vegetables can be tested after simple cleaning, crushing and filtering, and can be used The rapid auxiliary detection of 2,4‑D in complex fruit and vegetable samples has no restrictions on the types of samples to be tested; the detection time is short, easy to operate, and easy to carry; polyester fiber paper as the base not only reduces costs, but also Friendly to the environment.

Description

technical field [0001] The invention belongs to the technical fields of material science engineering and microfluidic chips, and in particular relates to a ratio-type fluorescent paper chip based on a polyester fiber film, a preparation method and an application. Background technique [0002] 2,4-Dichlorophenoxyacetic acid (2,4-D) is a phenoxyacetic acid plant growth regulator, which regulates metabolism by stimulating plant growth, and is often used in fruits and vegetables. However, because it is difficult to degrade and easily remains in fruits and vegetables, it may directly or indirectly enter the human body through the food chain and pose a threat to human health, so the detection of 2,4-D in fruits and vegetables is of great significance. [0003] The commonly used 2,4-D detection technology is high-performance liquid chromatography, but this method is difficult to popularize due to the precision and expensive equipment, long detection time, and complicated pretreatme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 张忠
Owner SHAANXI NORMAL UNIV
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