Specific protein marker for ovarian cancer diagnosis and preparation method of protein marker

A technology for protein labeling and ovarian cancer, applied in the field of biomedicine, to achieve strong maneuverability, high sensitivity and specificity, and strong reproducibility

Active Publication Date: 2019-12-20
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the shortcomings of existing ovarian cancer-specific protein markers, the purpose of the present invention is to provide a specific protein marker for ovarian cancer diagnosis and its preparation method , which is of great help to clinically guide the screening and diagnosis of ovarian cancer

Method used

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  • Specific protein marker for ovarian cancer diagnosis and preparation method of protein marker
  • Specific protein marker for ovarian cancer diagnosis and preparation method of protein marker
  • Specific protein marker for ovarian cancer diagnosis and preparation method of protein marker

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Embodiment Construction

[0032] One: The present invention is based on the mass spectrometry platform to detect the identification and screening of specific biological protein markers in ovarian cancer serum exosomes:

[0033] 1. Collection of experimental materials:

[0034]Sera were collected from normal healthy people, 9 patients with benign ovarian cystadenoma and 9 patients with malignant ovarian tumors. 5mL whole blood was collected using BD’s vacuum blood collection tube, centrifuged immediately at 3000rpm, transferred the supernatant to a new centrifuge tube, and frozen at minus 80°C.

[0035] 2. Main experimental methods:

[0036] TMT technology is a peptide in vitro labeling technology developed in recent years, which provides convenience for comparing the relative content of proteins in multiple groups of different samples at the same time. The invention conducts quantitative proteome research on samples by organically combining a series of cutting-edge technologies such as TMT labeling, ...

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Abstract

The invention relates to a specific protein marker for ovarian cancer diagnosis and a preparation method of the protein marker. The specific protein marker comprises the components including C reactive protein in serum exosome, solute transport protein family 11 member 2, ubiquitin-like PHD and ring finger domain protein 1. The preparation method comprises the following steps of protein extraction, pancreatin enzymolysis, TMT labeling, HPLC grading, liquid chromatography-mass spectrometry analysis, database search, protein identification, protein differential expression analysis, protein annotation and bioprotein marker screening. The protein marker has the advantages that (1) the marker has relatively high sensitivity and specificity; (2) ovarian malignant tumors can be distinguished frombenign tumors/healthy people; and (3) the preparation process is clear, the operability is high, and the repeatability is high.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a specific protein marker for ovarian cancer diagnosis and its preparation technology. Background technique [0002] Epithelial ovarian cancer (hereinafter referred to as ovarian cancer) is a common malignant tumor of the female reproductive system. Because of the difficulty in early diagnosis and poor treatment effect, its mortality rate ranks first among gynecological malignancies, even surpassing those of cervical cancer and endometrial cancer. Comprehensive, a serious threat to women's health. [0003] Intra-abdominal metastasis may occur in early ovarian cancer, and most patients do not have clinical symptoms until the late stage. Therefore, 75% of patients with ovarian cancer are already in the late stage when they are diagnosed, and only 25% of the patients are found in it. Advanced ovarian cancer ( The 5-year survival rate of patients with stage III~IV) is only 20%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574C07K14/47C12P21/06
CPCG01N33/6848G01N33/57449C07K14/4737C07K14/47
Inventor 杨国奋何伟鹏赖慧玲郭云云杨遵宪孙婷婷田黎明欧阳玲珑郑玉
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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