Method for detecting phenytoin sodium in blood

A phenytoin sodium and blood technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of low detection efficiency of blood samples, long detection time, and inability to remove impurities well, and achieves good sample recovery and sensitivity. High, suitable effect of internal standard quantification

Pending Publication Date: 2019-12-31
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology

Since the precipitated protein treatment cannot remove impurities well, the detection time is long, resulting in low detection efficiency of blood samples

Method used

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  • Method for detecting phenytoin sodium in blood
  • Method for detecting phenytoin sodium in blood
  • Method for detecting phenytoin sodium in blood

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Effect test

Embodiment 6

[0079] Embodiment 6: The recovery rate and precision of the method for detecting phenytoin sodium in blood

[0080] The standard koji intermediate solution containing phenytoin sodium was prepared into three concentrations of high, medium and low to carry out the sample recovery rate experiment and the precision experiment, and the detection was carried out according to the above-mentioned processing method and detection conditions, and the analysis was repeated for 3 batches. The recovery and precision are shown in Table 2 below:

[0081] Table 2

[0082] Add scalar 2500ng / mL 10000ng / mL 20000ng / mL The average recovery rate 101.72％ 95.27％ 94.31％ Precision RSD 3.39％ 1.94％ 1.95％

[0083] It can be seen from Table 2 that the average recovery rate of phenytoin sodium in the range of low, medium and high addition levels is 94.31%-101.72%, and the relative standard deviation is 1.94%-3.39%.

[0084] Based on the above-mentioned verification test...

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Abstract

A method for detecting phenytoin sodium in blood comprises the steps of: preparing a standard stock solution of phenytoin sodium, preparing a standard intermediate solution of phenytoin sodium, preparing an internal standard working solution, preparing a standard solution, detecting the standard solution by using a liquid chromatography-mass spectrometer, and fitting to obtain a standard curve equation corresponding to the phenytoin sodium, wherein the standard curve equation is as follows: y<1>=a*x<1>+b; processing blood to be detected, detecting the blood to be detected by using the liquid chromatogram-mass spectrometer, and calculating phenytoin sodium concentration in the blood to be detected. According to the method of the invention, the processing method of a blood sample to be detected and the selection of an internal standard substance enable the identification of the phenytoin sodium to be accurate, the analysis time is short, the interference is small, the internal standard quantification is suitable, the specificity is strong, the sensitivity is high, and meanwhile, all technical indexes such as a recovery rate, a detection limit and a precision meet the requirements, sothat the accuracy of a detection result is improved, and a system error is eliminated.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting phenytoin sodium in blood. Background technique [0002] Phenytoin is widely used clinically to treat generalized tonic-twin seizures, complex partial seizures (psychomotor seizures, temporal lobe epilepsy), simple partial seizures (localized seizures) and status epilepticus. Also indicated for the treatment of trigeminal neuralgia, recessive dystrophic epidermolysis bullosa, episodic choreoathetosis, episodic control disorders (hyperexcitability including rage, anxiety, and insomnia) disorders), myotonia, and cardiac conduction disorders in overdose of tricyclic antidepressants. It is also suitable for ventricular and supraventricular arrhythmias caused by digitalis poisoning, and has poor curative effect on arrhythmias caused by other causes. Therefore, the monitoring of phenytoin has important clinical significance. [0003] Currently, hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/04G01N30/72G01N30/86G01N30/06G01N30/14
CPCG01N30/04G01N30/06G01N30/14G01N30/72G01N30/8634G01N30/89G01N2030/042G01N2030/045G01N2030/062
Inventor 雒琴贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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