A method for improving the detoxification efficiency of Luo Han Guo seedlings

A technique for Luo Han Guo and seedlings is applied in the field of improving the detoxification efficiency of Luo Han Guo seeds and seedlings, which can solve the problems that the detoxification efficiency cannot be guaranteed, is not suitable for industrial production, and the detoxification culture method is cumbersome and complicated, and achieves low production cost and a culture method. Simple and efficient effect

Active Publication Date: 2021-10-08
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are certain defects in the above-mentioned detoxification methods. Whether it is the conventional shoot tip culture method or the use of detoxification medium for detoxification, it is only a one-time detoxification, and the detoxification efficiency cannot be guaranteed; The virus-free culture method is cumbersome, the production cost is high, and it is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of virus inhibitor culture medium:

[0023] The plant growth regulator 6-BA and NAA were added to the MS medium so that their final concentrations reached 0.3ppm and 0.03ppm respectively. Add ribavirin 10 ppm after autoclaving.

[0024] 2. Detoxification pretreatment of Luo Han Guo susceptible seedlings:

[0025] Take the 1cm terminal buds of the virus seedlings and transfer them to the virus inhibitor medium for subculture once, for 10 days each time. The culture conditions are: temperature 25°C, light intensity 33umol / s / m 2 , The light time is 12 hours a day. Then transfer it to 35° C. and take it out after 15 days for stripping off the shoot tip.

[0026] 3. Preparation and cultivation of shoot tips:

[0027] The virus seedlings treated with high temperature were taken out, and the 0.3mm shoot apical meristem was stripped under a stereomicroscope, and immediately after the shoot tip was stripped, it was placed in a medium without virus inhibitors f...

Embodiment 2

[0031] 1. Preparation of virus inhibitor culture medium:

[0032] The plant growth regulators 6-BA and NAA were added to the MS medium so that their final concentrations reached 0.7ppm and 0.07ppm, respectively. After autoclaving, 100 ppm of morpholinidine was added.

[0033] 2. Detoxification pretreatment of Luo Han Guo susceptible seedlings:

[0034] Take the 1cm terminal buds of the virus seedlings and transfer them to the virus inhibitor medium for subculture 5 times, each time for 30 days. The culture conditions are: temperature 30°C, light intensity 33umol / s / m 2 , The light time is 12 hours a day. Then it was transferred to 39° C. for 5 days and then taken out for stripping off the shoot tip.

[0035] 3. Preparation and cultivation of shoot tips:

[0036] The virus seedlings treated with high temperature were taken out, and the 0.3mm shoot apical meristem was stripped under a stereomicroscope, and immediately after the shoot tip was stripped, it was placed in a mediu...

Embodiment 3

[0040] 1. Preparation of virus inhibitor culture medium:

[0041] The plant growth regulators 6-BA and NAA were added to the MS medium so that their final concentrations reached 0.5ppm and 0.05ppm, respectively. Add Tamiflu 20ppm after autoclaving.

[0042] 2. Detoxification pretreatment of Luo Han Guo susceptible seedlings:

[0043] Take the 1cm terminal buds of the virus seedlings and transfer them to the virus inhibitor medium for subculture for 3 times, each time for 20 days. The culture conditions are: temperature 30°C, light intensity 33umol / s / m 2 , The light time is 12 hours a day. Then transfer it to 38.5° C. for 15 days and take it out for stripping off the shoot tip.

[0044] 3. Preparation and cultivation of shoot tips:

[0045] The virus seedlings treated with high temperature were taken out, and the shoot tip meristem of 0.2 mm was stripped under a stereomicroscope, and immediately after the shoot tip was stripped, it was placed in a medium without virus inhib...

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PUM

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Abstract

A method for improving the detoxification efficiency of Luo Han Guo seedlings, the method comprising: Step 1, preparing a rapid propagation medium for Luo Han Guo seedlings containing virus inhibitors; Step 2, transferring Luo Han Guo seedlings to the medium described in step 1 Subculture at 25-30°C; Step 3: Cultivate the Luo Han Guo seedlings in Step 2 at 35-39°C, and cut the stem tips of the seedlings; Step 4: Inoculate the Luo Han Guo seedlings in Step 3 into conventional rapid propagation The culture medium is cultured for 30-45 days to obtain detoxified Luo Han Guo plants; wherein, the virus inhibitor medium contains at least one of the following: ribavirin 10-60ppm, morpholinidine 100-500ppm, and Tamiflu 20-100ppm. The invention uses the virus inhibitor and the stem tip detoxification method in combination to carry out secondary detoxification on the grosvenoria seedlings, which significantly improves the detoxification efficiency of the grosvenoria seedlings. At the same time, the detoxification culture method of the present invention is simple, simplifies the operation procedure, has low production cost, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of plant propagation of grosvenoria grosvenori, in particular to a method for improving the detoxification efficiency of grosvenoria grosvenori seedlings. Background technique [0002] Luo Han Guo is the mature fruit of the Cucurbitaceae Luo Han Guo plant. It is mainly produced in Yongfu, Lingui and Longsheng counties in Guangxi. One of my country's traditional export commodities, it is very popular in Hong Kong, Macau, Southeast Asia, Europe and the United States. In recent years, the Luo Han Guo industry in Guangxi has developed rapidly, and the planting area of ​​Luo Han Guo has continued to expand. More and more companies have joined the ranks of the industrial production of Luo Han Guo tissue culture seedlings. However, due to various reasons, viral diseases are common in the main production areas of Luo Han Guo in Guangxi, and the incidence rate of severe plots reaches 100%, and the output of affected plants dec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张玉华莫雪燕兰玉
Owner GUILIN NATURAL INGREDIENTS CORP
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