Application of strain of trichoderma reesei to barley malt production

A technology of Trichoderma reesei and barley, applied in the preparation of malt, methods based on microorganisms, microorganisms, etc., can solve the problems of limited enzyme preparation application, low profit per ton of wine, etc., and achieve the effect of improving filtration performance

Active Publication Date: 2020-01-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

China Beer currently has a low overall profit per ton of wine, and the control of production

Method used

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  • Application of strain of trichoderma reesei to barley malt production
  • Application of strain of trichoderma reesei to barley malt production
  • Application of strain of trichoderma reesei to barley malt production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 Trichoderma reesei CICC41495 spore suspension

[0026] (1) Bacterial culture of solid slant test tube: Trichoderma reesei CICC41495 was inoculated into potato dextrose agar medium, and cultured at 30° C. for 7 days.

[0027] (2) Preparation of eggplant bottle: Inoculate a ring of spores of Trichoderma reesei CICC41495 from a solid slant test tube with an inoculation needle into a medium containing potato dextrose agar medium, and culture at 30° C. for 7 days.

[0028] (3) Collect the spores of Trichoderma reesei CICC41495 in the eggplant bottle with 0.9% NaCl solution to obtain a spore suspension.

Embodiment 2

[0029] Example 2 SDS-PAGE analysis of the arabinoxylan degrading enzyme system secreted by Trichoderma reesei CICC41495

[0030] The composition of enzymes secreted by Trichoderma reesei CICC41495 was analyzed by SDS-PAGE combined with matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry.

[0031] (1) SDS-PAGE analysis:

[0032] The types of enzyme proteins in the fermentation broth were analyzed by SDS-PAGE (the concentration of the stacking gel was 5%, and the concentration of the separating gel was 12.5%). After centrifugation, the enzyme solution and 5× sample buffer were mixed according to the ratio of 4:1, and the sample volume was 20uL. SDS-PAGE (the concentration of the stacking gel was 5%, and the concentration of the separating gel was 12.5%). First, the voltage was 60V until bromophenol blue Press it into a line and adjust the voltage to 80V until the bromophenol blue reaches the bottom of the rubber sheet. Remove the rubber sheet, f...

Embodiment 3

[0041] Example 3 Preparation of Jiangsu-produced barley malt with high filtration rate

[0042] (1) Barley soaking: add water to soak the screened barley.

[0043] The soaking process is as follows: the first step is wet soaking (soak the barley in the soaking water) for 12 hours, the second step is dry soaking (drain the soaking water and keep the surface of the barley moist) for 6 hours, and the third step Wet immersion for 16 hours, the fourth step is dry immersion for 12 hours;

[0044] The temperature during the first wet immersion and dry immersion was 16°C. During the third step of wet immersion, the spores of Trichoderma reesei CICC41495 were inoculated in the soaking water so that the concentration of spores in the soaking water after inoculation was 1.5×10 7 per mL, the temperature of barley soaking water was increased to 28°C to promote the growth of Trichoderma reesei CICC41495 on the surface of barley and the secretion of enzymes.

[0045] (2) Germination: the m...

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Abstract

The invention relates to application of a strain of trichoderma reesei to barley malt production. The trichoderma reesei CICC41495 is used for treating Jiangsu barley malt; the content of high-molecular-weight arabinoxylan in the agreement wort of the treated barley malt is reduced from 876 mg/L to 235 mg/L, and is reduced by 73.2 percent; the viscosity is reduced from 1.59 mPa.s to 1.43 mPa.s, and is reduced by 10.1 percent; and the filtering speed is accelerated from 3.5 mL/min to 6.2mL/min, and is improved by 77 percent. The method provided by the invention has the advantages that by usingthe barley sprouting environment conditions, the trichoderma reesei grows on the surface of the barley; meanwhile, an araboxylan degrading enzyme system is secreted; the degradation of the high-molecular-weight arabinoxylan in the Jiangsu barley malt is promoted; the filtering speed of the agreement wort of the Jiangsu barley malt is accelerated; and conventional physicochemical indexes reach thelevel of first-grade products in the industrial standard QB/T 1686-2008.

Description

technical field [0001] The invention relates to the application of a strain of Trichoderma reesei in barley malt production and belongs to the technical field of malt production. Background technique [0002] In beer saccharification production, the saccharification mash is separated through the filter tank to obtain transparent and clear wort, and the separation speed of the wort in the saccharification mash is the filtration speed. The filtration rate of barley malt has an important influence on the beer production efficiency and the quality of finished beer. Therefore, the filtration rate of wort is one of the most important indicators for brewers, which determines the efficiency of the beer production process. Some studies have found that the arabinoxylan in the milk cell wall affects the filtration speed. Due to the high viscosity of high molecular weight arabinoxylan aqueous solution, the filtration speed of barley malt is slow, and the viscosity of saccharification i...

Claims

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Application Information

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IPC IPC(8): C12C1/02C12C1/027C12C1/067C12R1/885
CPCC12C1/02C12C1/027C12C1/067
Inventor 孙军勇陆健田甜甜王茂章颜义勇商曰玲
Owner JIANGNAN UNIV
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