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Application of pdia3p1 as a prognostic marker in glioma

A technology for prognostic markers and glioma, which is applied in the determination/testing of microorganisms, medical preparations containing active ingredients, antineoplastic drugs, etc. It can solve the problems of invasion and reduce HCC cell migration, etc.

Active Publication Date: 2020-11-24
SHANDONG UNIV QILU HOSPITAL
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  • Description
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AI Technical Summary

Problems solved by technology

In 2017, Kong et al. found that knockdown of PDIA3P1 can reduce the migration, invasion and proliferation of HCC cells by affecting the p53 pathway, but the detailed mechanism has not been studied in detail

Method used

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  • Application of pdia3p1 as a prognostic marker in glioma
  • Application of pdia3p1 as a prognostic marker in glioma
  • Application of pdia3p1 as a prognostic marker in glioma

Examples

Experimental program
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Effect test

Embodiment 11

[0115] Example 1 1. Materials and methods

[0116] 1.1 Data set acquisition

[0117] The microarray data set uses the Illumina Human HT-12 V3.0 platform to download the public U87MG glioma cell line microarray gene map data set GSE45301 from Gene Expression Omnibus (GEO). The RNA sequencing data of tumor tissue samples were obtained from TCGA (https: / / cancergenome.nih.gov) and CGGA (http: / / www.cgga.org.cn / ), respectively. The cutoff values ​​for high and low PDIA3P1 expression were set as the expression level of the median sample. Clinical attributes are downloaded from the TCGA dataset. Enrichment analysis The gene expression profiles in the TCGA dataset were correlated with the biological process (BP) of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and PDIA3P1. Differentially expressed genes (DEGs) were detected with the DESeq2 package of R, and the genes with significant differences were defined as |log2FC|≥2, p<0.05. DEGs were enriched and analyze...

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Abstract

The invention belongs to the technical field of a prognostic marker, and particularly relates to an application of PDIA3P1 as a glioma prognostic marker. The research disclosed by the invention investigates a mechanism of the PDIA3P1 in MES transformation of low-oxygen induced glioma, confirms that the expression of the PDIA3P1 and the tumor level of glioma patients, and transcriptome subtypes aretightly associated with prognosis. The PDIA3P1 is used as ceRNA, through miR-124-3p, the RELA expression is regulated, and a downstream NF-KB route is activated, so that the MES transformation of glioma cells is promoted. In addition, the prognostic marker also confirms that a low-oxygen induction factor 1 directly combines with a PDIA3P1 promoter region and the transcript is activated. The PDIA3P1 can achieve the key connection effect between low oxygen and MES transformation of the glioma through a PDIA3P1-miR-124-3p-RELA shaft, and can be used as a prognostic indicator and a potential treatment target point of the glioma.

Description

technical field [0001] The disclosure belongs to the technical field of glioma prognostic markers, and specifically relates to the application of PDIA3P1 as a glioma prognostic marker. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Human gliomas account for 75% of malignant primary brain tumors in adults. Although treatments including extensive resection, radiotherapy and adjuvant temozolomide have significantly improved the prognosis of glioma patients, they still lag behind other tumors. Since the identification of new biomarkers can affect the biological characteristics of tumors, glioma genotypes can provide more accurate diagnostic information in early diagnosis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 李刚薛皓王劭博亓彦华邓林赵荣荣薛付忠杨帆许野平张传锋
Owner SHANDONG UNIV QILU HOSPITAL
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