Cost-effective compositions and methods for enhanced aquatic farming and aquarium fish husbandry
A technology of aquaculture and composition, applied to the cultivation of edible or ornamental fish and other aquatic organisms, the materials for cultivating fish, improving the field of aquaculture and aquaculture, which can solve the problems of reducing the quality of fish and the possibility of commercial use, etc., to achieve Cost-effective, easy-to-operate effect
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Embodiment 1
[0168] Example 1 - Cultivation of Wickerhamomyces anomalus and Pseudozyma aphidis
[0169] Wickhamiana anomaly was grown in a reactor with a working volume of 800 L for the production of biomass and sophorolipids under non-sterile conditions. Use the method of sanitizing empty containers, in which the inner surfaces are treated with 2-3% hydrogen peroxide and rinsed with bleach and high-pressure hot water. The media components containing all the necessary components are decontaminated at 85-90°C or dissolved in 3% hydrogen peroxide. The fermentation temperature is kept between 25-30°C. The pH starts at 5.0-5.5 and drops to 3.0-3.5 where it remains stable. Biomass production can be achieved after about 48 hours of fermentation.
[0170] After 7 to 9 days of fermentation, sophorolipids accumulate. After the fermentation is complete, the culture containing the biomass and, if applicable, a low concentration of sophorolipids can be applied, for example, to a pond or tank conta...
Embodiment 2
[0172] Example 2 - Compositions for fish feeding and fish farm treatment
[0173] Contains glucose, rapeseed oil, yeast extract, NH 4 Cl, KH 2 PO 4 .H 2 O and MgSO 4 .7H 2 Both the treatment product and the feed product can be obtained in the culture medium of O. The initial pH was adjusted to about 5.5 with KOH. Cultures were grown at about 25°C without stabilization.
[0174] Saccharomyces aeruginosa and Toructus aphidii were cultured in separate tanks. After the fermentation process is complete, the culture is inactivated by inactivating the culture at pasteurization temperatures (up to 65 to 70°C for a duration sufficient to inactivate 100% of the yeast cells) and raising the pH to about 10.0-12.0, thereby Prepare the composition. This causes partial hydrolysis of the cells and allows some nutrients to be released therein. The composition is then neutralized to a pH of approximately 6.5–7.5 and the various hydrolyzed ingredients mixed.
[0175] The resulting mic...
Embodiment 3
[0176] Example 3 - Fish Food
[0177]A substantial part or all of the fish meal can be replaced by the composition of the invention. In one embodiment, air drying containing yeast biomass (e.g., W. anomalus, P. aphidis, Candida globosa (S. bombicola) and / or Candida (C.apicola)) and optionally purified biosurfactant to produce fish feed pellets. Granules can be mixed with traditional fishmeal in a ratio of eg 1:1, 2:1 or 1:2. These pellets can also be used to completely replace traditional fishmeal if desired. The yeast and biosurfactant-based food compositions of the present invention protect food from contamination by microbial pathogens, as well as potential disease in fish subsequently consuming the food. The composition may also increase the bioavailability of nutrients to the fish ingesting it, resulting in an overall healthier fish.
[0178] A diet containing 50% yeast single-cell protein instead of fishmeal provided the best growth response in terms of percent weigh...
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