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Method for separating and purifying high-purity flavonoid glycoside compounds from gynura procumbens stems

A separation and purification technology of chrysanthemum notoginseng, which is applied in the field of natural products, can solve the problems of large solvent consumption, large sample loss, and limited conditions, and achieve the effect of low overall cost, small sample loss, and mild extraction conditions

Active Publication Date: 2020-02-21
江西省蔓三七健康科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no relevant report on the isolation of flavonoids from the stems of Pingbu notoginseng
[0004] In the prior art, methods such as traditional column chromatography separation and recrystallization are mostly used to extract and separate flavonoids. These methods require repeated column chromatography separation, complicated operation, limited conditions, long time consumption, large solvent consumption, and Large sample loss, poor effect

Method used

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  • Method for separating and purifying high-purity flavonoid glycoside compounds from gynura procumbens stems

Examples

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Effect test

Embodiment 1

[0027] A method for isolating and purifying high-purity flavonoid glycosides from the stems of Panwort notoginseng, comprising the following specific steps:

[0028] (1), get the stem 1.0kg of the chrysanthemum notoginseng stem of natural drying in the shade, pulverize, carry out ultrasonic extraction with the ethanol of volume concentration 75%, the solid-liquid ratio of the chrysanthemum notoginseng stem powder and the ethanol solution is 1g: 15mL, the extraction time is 2h, the ultrasonic power is 350W, and then filtered, and the filter residue is treated twice; the filtrate is combined and concentrated under reduced pressure until there is no ethanol smell, and the total flavonoid extract is obtained;

[0029] (2), the total flavonoids extract that obtains is absorbed and enriched through HPD-400 macroporous adsorbent resin, first with the deionized water elution of 8 times of column volumes, then successively use the volume concentration of 6 times of column volumes to be ...

Embodiment 2

[0034] A method for isolating and purifying high-purity flavonoid glycosides from the stems of Panwort notoginseng, comprising the following specific steps:

[0035] (1), get 1.5kg of the stem of chrysanthemum notoginseng dried naturally in the shade, pulverize, carry out ultrasonic extraction with the ethanol of volume concentration 70%, the solid-liquid ratio of the stem of chrysanthemum notoginseng powder and ethanol solution is 1g: 20mL, the extraction time is 2h, the ultrasonic power is 350W, and then filtered, and the filter residue is treated twice; the filtrate is combined and concentrated under reduced pressure until there is no ethanol smell, and the total flavonoid extract is obtained;

[0036] (2), the obtained total flavone extract is absorbed and enriched by AB-8 macroporous adsorption resin, first eluted with deionized water of 8 times of column volume, then successively with 6 times of column volume at a volume concentration of 20%, 40 %, 60%, 80%, and 90% etha...

Embodiment 3

[0041] A method for isolating and purifying high-purity flavonoid glycosides from the stems of Panwort notoginseng, comprising the following specific steps:

[0042] (1), get 2.0kg of the stem of chrysanthemum notoginseng dried naturally in the shade, pulverize, carry out ultrasonic extraction with the ethanol of volume concentration 80%, the solid-liquid ratio of the stem of chrysanthemum notoginseng powder and ethanol solution is 1g: 10mL, the extraction time is 3h, the ultrasonic power is 350W, and then filtered, and the filter residue is treated twice; the filtrate is combined and concentrated under reduced pressure until there is no ethanol smell, and the total flavonoid extract is obtained;

[0043] (2), the obtained total flavonoids extract is enriched by D-101 macroporous adsorption resin adsorption, first eluted with deionized water of 8 times of column volume, and then successively with 6 times of column volume with a volume concentration of 20%, 40 %, 60%, 80%, and ...

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Abstract

The invention belongs to the technical field of natural products, and discloses a method for separating and purifying high-purity flavonoid glycoside compounds from gynura procumbens stems, which comprises the following steps: preparing a total flavonoid extracted solution; enriching the total flavonoids; separating and purifying by high-speed counter-current chromatography; separating and purifying by preparative chromatography; wherein the purity of obtained quercetin, luteolin, apigenin, tetrahydroxy flavone and 7-methyl ether baicalein is 98% or above. According to the method, five flavonoid glycoside compound monomers can be separated and purified from the gynura procumbens stems in one time, and the extraction efficiency, the separation purity and the yield of flavonoid glycoside compounds in the gynura procumbens stems are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of natural products, relates to the technical field of extraction and separation of effective components of natural products, and in particular relates to a method for separating and purifying high-purity flavonoid glycosides from the stem of chrysanthemum notoginseng. Background technique [0002] Gynura procumbens (Lour.) Merr. is a plant of the genus Panax notoginseng in the family Asteraceae, also known as panax notoginseng, snake bone, continuation of life grass, fairy grass, pungent, slightly bitter, and cool in nature. It is a perennial herb with both medicinal and edible uses, widely distributed in countries such as China, Malaysia, Thailand, Indonesia, South Korea and the Philippines. Chrysanthemum notoginseng has a wide range of pharmacological effects. In the first half of 2009, relevant units such as China Center for Disease Control and Prevention, National Food Quality Supervision and Inspection...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 胡居吾韩晓丹吴磊涂招秀李雄辉
Owner 江西省蔓三七健康科技有限公司
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