Automatic cell culture device for mouse uterine stroma

A technology of cell culture and uterus, which is applied in the field of cell culture, can solve the problems of easily damaged cells and tissues, and achieve the effect of preventing damage to the human body, preventing pollution, and good mixing effect

Active Publication Date: 2020-02-21
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cell culture device in the prior art needs to rely on mechanical stirring to stir the culture medium, which is easy to damage the cell tissue

Method used

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  • Automatic cell culture device for mouse uterine stroma
  • Automatic cell culture device for mouse uterine stroma
  • Automatic cell culture device for mouse uterine stroma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as Figure 1-12 As shown, an automated cell culture device for mouse uterine matrix, including an incubator 1, a case cover 2, a petri dish holder 3, an up and down shaking mechanism 4, a rotating linkage mechanism 5, a left and right shaking mechanism 6 and a drive motor 7 , the drive motor 7 is installed on the left and right shaking mechanism 6 and is connected to the left and right shaking mechanism 6 by transmission; the left and right shaking mechanism 6 is connected to the incubator 1 by transmission; the incubator 1 is connected to the Cover 2; the up and down shaking mechanism 4 is movably connected in the incubator 1; the left and right shaking mechanism 6 is transmission connected to the up and down shaking mechanism 4; the culture dish holder 3 is connected to the up and down shaking mechanism 4 The up and down shaking mechanism 4 is connected to the rotation linkage mechanism 5; the rotation linkage mechanism 5 is connected to the incubator 1; the rota...

Embodiment 2

[0036] Such as Figure 1-12 As shown, the incubator 1 includes a rectangular box body 110 with an open top surface and an insertion rod locking member 120; the middle end of the front side of the rectangular box body 110 is sealed and fixedly connected with a transparent observation window; the insertion rod locking member 120 Including unlocking pull rod 120A, fixed rod seat 120B, locking insert rod 120C, compression spring 120D and movable ring 120E; there are two fixed rod seats 120B, and the two fixed rod seats 120B are symmetrically fixed on the left and right sides of the incubator 1 On the side; the two fixed rod seats 120B slide to match the middle part of a locking insertion rod 120C respectively, and the rear ends of the two locking insertion rods 120C are fixed on the unlocking rod 120A, and the unlocking rod 120A is blocked by the incubator 1 on the back side of the rear side; the front ends of the two locking rods 120C are respectively plugged and connected in the...

Embodiment 3

[0038] Such as Figure 1-12 As shown, the left and right shaking mechanism 6 includes a mounting frame 610, a lead screw 1 620, a guide shaft 630 and a rack 1 640; the driving motor 7 is fixed on the mounting frame 610 through a motor bracket; the output of the driving motor 7 The end drive is connected to the lead screw one 620; the two ends of the lead screw one 620 are respectively rotatably connected to the two ends of the mounting frame 610; the two ends of the guide shaft 630 are respectively fixed on the two ends of the mounting frame 610; the rack One 640 is fixed in the middle of the mounting frame 610; the lead screw one 620 is threadedly fitted on the incubator 1; the guide shaft 630 is slidingly fitted on the incubator 1; the rack one 640 engages the transmission Connect the up and down rocking mechanism 4. The leading screw 1 620 in the left and right shaking mechanism 6 rotates under the drive of the drive motor 7. When the leading screw 620 rotates, it can driv...

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Abstract

The invention relates to the technical field of cell culture, and in particular relates to an automatic cell culture device for mouse uterine stroma. The device comprises a culture box, a box cover, aculture dish holder, an up-down shaking mechanism, a rotation linkage mechanism, a left-right shaking mechanism and a driving motor, and the driving motor is installed on the left-right shaking mechanism and is in transmission connection with the left-right shaking mechanism; the left-right shaking mechanism is in transmission connection with the culture box; the upper part of the culture box isconnected with the box cover; the up-down shaking mechanism is movably connected to the interior of the culture box; the left-right shaking mechanism is in transmission connection with the up-down shaking mechanism; and the culture dish holder is connected to the up-down shaking mechanism. By using cooperation of the up-down shaking mechanism, the rotation linkage mechanism and the left-right shaking mechanism, a culture dish filled with cells and a culture solution on the culture dish holder is shaken and mixed uniformly to avoid damage to cells caused of mixing and stirring of the cells anda culture solution by a mechanical stirring mechanism.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an automatic cell culture device for mouse uterine matrix. Background technique [0002] Cell culture is a common technique involved in medical research. It is known that various stimuli such as pressure, oxygen content, temperature, etc. can have an important impact on the biological behavior of cells, such as affecting cell proliferation, apoptosis, phenotype conversion, and so on. The establishment of a simple and safe laboratory controlled oxygen-controlled temperature-controlled air pressure cell culture device is helpful for studying the biochemical reactions of various cells under different pressures, hypoxia / hypoxia, and oxygen-enriched conditions. Various reaction conditions can be applied separately to study the molecular mechanism of cell changes under a single factor; different conditions can also be applied simultaneously or sequentially to study the molecular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06C12M3/00C12M1/12C12M1/04
CPCC12M23/38C12M23/48C12M27/16C12M29/04
Inventor 张晋平王俊龙丁艳平冯汉青杨宁丁兰王玮郑晟程昉程晓
Owner NORTHWEST NORMAL UNIVERSITY
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