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Method for promoting the germination of seeds of trees of the genus Cereus and Paphiopedilum by fungi of the genus Cereus
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A technology of plant seeds and Paphiopedilum, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problem of no fungus of the genus Cereus
Active Publication Date: 2021-04-13
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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[0009] At present, in the prior art, there is no report on the establishment of a symbiotic system between Sebacina sp., Epidendrum secundum and Paphiopedilum Pacific Shamrock.
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[0019] The method that Cereus fungus of the present invention promotes the germination of tree orchids and Paphiopedilum seeds, its preferred embodiment is:
[0020] include:
[0021] The 3-1 strain is used to establish a symbiotic relationship with tree orchid and Paphiopedilum seeds at the same time to promote plant seed germination;
[0022] The Latin name of described tree orchid: Epidendrumsecundum, the Latin name of described Paphiopedilum: Paphiopedilum Pacific Shamrock;
[0023] The 3-1 strain is a fungus of the genus Cerecia, its Latin name: Sebacinasp., strain number: 3-1, ITS gene NCBI registration number: MN644843, CGMCC preservation number: CGMCC No.18583.
[0024] The culture medium for the 3-1 strain to promote the seed germination of Shulan and Paphiopedilum is: OMA with a concentration of 2.0 g / L.
[0025] The establishment time of the symbiotic relationship between the tree orchid seeds and the 3-1 strain at 2.0 g / L OMA was 21 days, and the germination rate...
specific Embodiment
[0029] 1. Test material
[0030] (1) Plant materials: seeds of Epidendrum secundum and Paphiopedilum Pacific Shamrock.
[0031] (2) Fungal material: a fungus of the genus Sebacina sp. isolated and obtained in our laboratory, strain number: 3-1. ITS gene NCBI registration number: MN644843. CGMCC deposit number: CGMCC No.18583.
[0032] 2. Test method (symbiotic germination test of Orchidaceae plant seeds and fungus of the genus Colloides)
[0033] (1) Configuration of culture medium
[0034] The configuration of symbiotic medium (oat medium OMA): take oatmeal and grind it into powder, take three gradients of oat flour respectively, and prepare 0.5g / L, 1.0g / L, 2.0g / L, 3.0g / L , 4.0 g / L OMA solution. Boil in deionized water for 1 hour, filter, add 8.0 g of agar, add deionized water to the filtrate to 1000 mL, and sterilize at high temperature.
[0035] The configuration of the fungal culture medium: 200g peeled potatoes, cut into small pieces of 0.5-1cm square, add 800mL of ...
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Abstract
The invention discloses a method for promoting the germination of Epidendrum secundum and Paphiopedilum seeds by fungi of the genus Cereus, using 3‑1 bacterial strain (Sebacina sp.) to simultaneously establish symbiosis with the seeds of Epidendrum secundum and Paphiopedilum relationship, to promote the germination of plant seeds, the medium is: OMA with a concentration of 2.0g / L. It took 21 days for the establishment of symbiotic relationship between tree orchid seeds and 3-1 strain in 2.0g / L OMA, and the germination rate was 83.35±0.88%; After 25 days, the germination rate was 66.23±2.55%. Screen the symbiotic medium and culture conditions that can effectively promote the seed germination of different Paphiopedilum species, and use the symbiotic germination culture technology to improve the seed germination rate of Tree Orchid and Paphiopedilum, and establish the The symbiotic system of fungi is of great significance to the effective protection of Paphiopedilum.
Description
technical field [0001] The present invention relates to a kind of technology of promoting seed germination of Epidendrum secundum and Paphiopedilum, in particular to a kind of Sebacina sp. (Paphiopedilum) seed germination method. Background technique [0002] Orchidaceae (Orchidaceae) belongs to the class Monocots of the angiosperm phylum, and there are about 700 genera and more than 35,000 species distributed in the world (Dressler, 1993; Cribb, 2003), and its number of species ranks second only to Compositae. Branch (Lang Kaiyong, 1994). It is beautiful in shape, bright in color, and has great ornamental and medicinal value. [0003] Orchidaceae has two notable characteristics: one, the embryo of orchidaceae seed is only made up of dozens of cells, and differentiation is incomplete, and it is difficult to germinate naturally. 2. Orchidaceae are almost all mycorrhizal plants. In the natural growth state, no matter green leaf orchid or non-green leaf orchid, in its life h...
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