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Mass spectrometric detection method for plasma or tissue concentrations of seven components of compound salvia miltiorrhiza preparation

A compound salvia miltiorrhiza and detection method technology, which is applied in the directions of measuring devices, material separation, and analysis of materials, can solve problems such as difficult handling and detection, large content differences, and cumbersome steps, and achieve good practical significance, high separation, and Analyze quick results

Active Publication Date: 2020-03-24
GUANGZHOU PHARMACEUTICAL INDUSTRIAL RESEARCH INSTITUTE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the large difference in properties of the seven components of the compound Danshen preparation, water-soluble components (salvianolic acid B, danshensu, notoginsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rb 1 ) and fat-soluble components (tanshinone I and tanshinone IIA), and the content is quite different, so it is difficult to use a pretreatment method to process and detect
As a result, the current research on the detection method of the main components of the compound Danshen preparation usually adopts the method of pretreatment and detection of different components separately, the process is complicated and the steps are cumbersome

Method used

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  • Mass spectrometric detection method for plasma or tissue concentrations of seven components of compound salvia miltiorrhiza preparation
  • Mass spectrometric detection method for plasma or tissue concentrations of seven components of compound salvia miltiorrhiza preparation
  • Mass spectrometric detection method for plasma or tissue concentrations of seven components of compound salvia miltiorrhiza preparation

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Experimental program
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preparation example Construction

[0032] (1) Preparation of stock solution:

[0033] Take standard tanshinone I, tanshinone IIA, salvianolic acid B, danshensu, notoginseng saponin R 1 , Ginsenoside Rg 1 and Rb 1 , respectively dissolved in (80 ± 5)% aqueous methanol solution to obtain a standard stock solution; the standard stock solution was mixed to obtain a mixed standard stock solution;

[0034] Get carbamazepine and be dissolved in (80 ± 5)% methanol aqueous solution, make carbamazepine stock solution;

[0035] (2) Preparation of working solution:

[0036] Dilute the mixed standard stock solution with (80 ± 5)% aqueous methanol solution respectively to prepare a mixed standard curve sample working solution and a mixed quality control sample working solution;

[0037] Dilute the carbamazepine stock solution with (80 ± 5)% aqueous methanol to prepare an internal standard working solution;

[0038] (3) Preparation of the sample solution to be tested:

[0039] Preparation of the plasma sample solution t...

Embodiment 1

[0092] (1) Preparation of solution

[0093] Preparation of stock solutions:

[0094] Weigh an appropriate amount of the main components of the compound danshen preparation, tanshinone I, tanshinone IIA, salvianolic acid B, danshensu, notoginseng saponin R 1 , Ginsenoside Rg 1 , Ginsenoside Rb 1 Standards were dissolved in an appropriate amount of 80% methanol aqueous solution to prepare standard stock solutions with a concentration of 1.00 mg / mL. Mix the seven standard stock solutions with concentrations of 1.00 mg / mL in equal volumes, and add an appropriate amount of 80% aqueous methanol solution to prepare a mixed standard stock solution with the concentrations of the seven components at 0.100 mg / mL.

[0095] Take an appropriate amount of carbamazepine standard substance and dissolve it in 80% aqueous methanol to prepare a carbamazepine stock solution with a concentration of 1.00 mg / mL.

[0096] Preparation of standard curve working solution:

[0097] Dilute the seven c...

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Abstract

The invention relates to a mass spectrometric detection method for plasma or tissue concentrations of seven components of a compound salvia miltiorrhiza preparation. The method comprises the followingsteps of (1) preparing a stock solution, namely respectively dissolving standard substances tanshinone I, tanshinone IIA, salvianolic acid B, tanshinol, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in a (80 + / -5)% methanol aqueous solution to prepare the standard substance stock solution; dissolving carbamazepine in a (80 + / -5)% methanol aqueous solution to prepare a carbamazepine stock solution; (2) preparing a working solution; (3) preparing a to-be-detected sample solution; (4) carrying out liquid chromatography separation on the to-be-detected sample solution obtained in the step (3); and carrying out mass spectrometric analysis on the sample after liquid chromatography separation. In the detection process of the method, the chromatographic peak shape is excellent, a channel of each compound only has a unique target peak, the interference of other effects, such as solvent effects, etc., is avoided, and the method is easy to operate and rapid to analyze, and is high in specificity and high in separation degree.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a mass spectrometry detection method for plasma or tissue concentrations of seven components of a compound salvia miltiorrhiza preparation. Background technique [0002] Compound Danshen preparation is composed of 3 traditional Chinese medicines of Danshen, Panax notoginseng and Borneol. There are 9 dosage forms: Compound Danshen Oral Liquid, Compound Danshen Aerosol, Compound Danshen Buccal Tablets, Compound Danshen Capsules, Compound Danshen Tablets, Compound Danshen Granules, Compound Danshen Dropping pills, compound danshen spray, compound danshen soft capsules. Compound Danshen preparation has the effects of promoting blood circulation and removing blood stasis, regulating qi and relieving pain. It is used for chest obstruction caused by stagnation of qi and blood stasis, such as chest tightness, tingling pain in the precordial area, and angina pectoris of coronary ath...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06
Inventor 殷玮孙晶晶凌珊周兰华胡钟芳谢瑞吴家欣戚园梅张尧君彭佳裕蔡文镇
Owner GUANGZHOU PHARMACEUTICAL INDUSTRIAL RESEARCH INSTITUTE
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