Raspberry leaf alcohol extract biopesticide and preparation method thereof
A biopesticide and raspberry technology, applied in botany equipment and methods, plant growth regulators, biocides, etc., can solve the problems of high toxicity, drug resistance, and low efficacy, and achieve considerable results
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Embodiment 1
[0027] The present embodiment provides a kind of biopesticide of raspberry leaf ethanol extract, comprising raspberry leaf ethanol extract, sapinberry oil, sapinberry saponin, chitosan and sodium carbonate, and the specific formula is: sapinberry oil content 3% (m / V), alcohol extract of raspberry leaves 8% (m / V), Sapindus saponin 2% (m / V), chitosan 2% (m / V), sodium carbonate 2% ( m / V), and the balance is water.
[0028] Wherein, the preparation method of raspberry leaf ethanol extract:
[0029] a) Pulverization: the raspberry leaves are blast-dried at 50° C., and pulverized after drying to obtain raspberry leaf powder;
[0030] b) Hemicellulase treatment of raspberry leaf powder: put raspberry leaf powder into water, and add hemicellulase of 2% by weight of raspberry leaf powder in the water, and enzymolyze it at 50°C for 40 minutes to obtain enzymatic hydrolysis After the raspberry leaf powder.
[0031] c) Ethanol extraction: filter the raspberry leaf powder after enzymoly...
Embodiment 2
[0041] A biopesticide of raspberry leaf ethanol extract, comprising raspberry leaf ethanol extract, sapinberry oil, sapinberry saponin, chitosan and sodium carbonate, and the specific formula is: the content of sapinberry oil is 1% (m / V), raspberry leaf alcohol extract 5% (m / V), Sapindus saponin 1% (m / V), chitosan 0.5% (m / V), sodium carbonate 1% (m / V), The balance is water.
[0042] Wherein, the preparation method of raspberry leaf ethanol extract:
[0043] a) Pulverization: the raspberry leaves are blast-dried at 80° C., and pulverized after drying to obtain raspberry leaf powder;
[0044] b) Treatment of raspberry leaf powder with hemicellulase: put raspberry leaf powder into water, and add hemicellulase with 5% weight of raspberry leaf powder in the water, and enzymolyze it at 60°C for 60 minutes to obtain enzymatic hydrolysis After the raspberry leaf powder.
[0045] c) Ethanol extraction: filter the raspberry leaf powder after enzymolysis, wash and drain, add 75% (v / v...
Embodiment 3
[0054] A biopesticide of raspberry leaf ethanol extract, comprising raspberry leaf ethanol extract, sapinberry oil, sapinberry saponin, chitosan and sodium carbonate, and the specific formula is: sapinberry oil content 5% (m / V), raspberry leaf alcohol extract 15% (m / V), Sapindus saponin 4% (m / V), chitosan 3% (m / V), sodium carbonate 2.5% (m / V), The balance is water.
[0055] Wherein, the preparation method of raspberry leaf ethanol extract:
[0056] a) Pulverization: the raspberry leaves are blast-dried at 30° C., and pulverized after drying to obtain raspberry leaf powder;
[0057] b) Treatment of raspberry leaf powder with hemicellulase: put raspberry leaf powder into water, add hemicellulase at 0.2% by weight of raspberry leaf powder to the water, and enzymolyze it at 30°C for 10 minutes to obtain enzymatic hydrolysis After the raspberry leaf powder.
[0058] c) Ethanol extraction: filter the raspberry leaf powder after enzymolysis, wash and drain, add 75% (v / v) aqueous ...
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