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A kind of raspberry leaf ethanol extract biopesticide and preparation method thereof

A biopesticide and raspberry technology, applied in botany equipment and methods, plant growth regulators, biocides, etc., can solve the problems of high toxicity, low efficacy, drug resistance, etc., and achieve considerable results

Active Publication Date: 2020-11-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the safety problems such as high toxicity of traditional pesticides and long-term use with drug resistance, the advantages of natural biological pesticides are becoming more and more significant. Raspberry leaf alcohol extract biopesticide, the formula of the raspberry leaf alcohol extract biopesticide is: raspberry leaf alcohol extract 5~15% (m / V), Sapinberry oil content 1~5% (m / V), Sapindus saponin 1~4% (m / V), chitosan 0.5~3% (m / V), sodium carbonate 1~2.5% (m / V), the balance is water

Method used

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  • A kind of raspberry leaf ethanol extract biopesticide and preparation method thereof

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Effect test

Embodiment 1

[0027] The present embodiment provides a kind of biopesticide of raspberry leaf ethanol extract, comprising raspberry leaf ethanol extract, sapinberry oil, sapinberry saponin, chitosan and sodium carbonate, and the specific formula is: sapinberry oil content 3% (m / V), alcohol extract of raspberry leaves 8% (m / V), Sapindus saponin 2% (m / V), chitosan 2% (m / V), sodium carbonate 2% ( m / V), and the balance is water.

[0028] Wherein, the preparation method of raspberry leaf ethanol extract:

[0029] a) Pulverization: the raspberry leaves are blast-dried at 50° C., and pulverized after drying to obtain raspberry leaf powder;

[0030] b) Hemicellulase treatment of raspberry leaf powder: put raspberry leaf powder into water, and add hemicellulase of 2% by weight of raspberry leaf powder in the water, and enzymolyze it at 50°C for 40 minutes to obtain enzymatic hydrolysis After the raspberry leaf powder.

[0031] c) Ethanol extraction: filter the raspberry leaf powder after enzymoly...

Embodiment 2

[0041] A biopesticide of raspberry leaf ethanol extract, comprising raspberry leaf ethanol extract, sapinberry oil, sapinberry saponin, chitosan and sodium carbonate, and the specific formula is: the content of sapinberry oil is 1% (m / V), raspberry leaf alcohol extract 5% (m / V), Sapindus saponin 1% (m / V), chitosan 0.5% (m / V), sodium carbonate 1% (m / V), The balance is water.

[0042] Wherein, the preparation method of raspberry leaf ethanol extract:

[0043] a) Pulverization: the raspberry leaves are blast-dried at 80° C., and pulverized after drying to obtain raspberry leaf powder;

[0044] b) Treatment of raspberry leaf powder with hemicellulase: put raspberry leaf powder into water, and add hemicellulase with 5% weight of raspberry leaf powder in the water, and enzymolyze it at 60°C for 60 minutes to obtain enzymatic hydrolysis After the raspberry leaf powder.

[0045] c) Ethanol extraction: filter the raspberry leaf powder after enzymolysis, wash and drain, add 75% (v / v...

Embodiment 3

[0054] A biopesticide of raspberry leaf ethanol extract, comprising raspberry leaf ethanol extract, sapinberry oil, sapinberry saponin, chitosan and sodium carbonate, and the specific formula is: sapinberry oil content 5% (m / V), raspberry leaf alcohol extract 15% (m / V), Sapindus saponin 4% (m / V), chitosan 3% (m / V), sodium carbonate 2.5% (m / V), The balance is water.

[0055] Wherein, the preparation method of raspberry leaf ethanol extract:

[0056] a) Pulverization: the raspberry leaves are blast-dried at 30° C., and pulverized after drying to obtain raspberry leaf powder;

[0057] b) Treatment of raspberry leaf powder with hemicellulase: put raspberry leaf powder into water, add hemicellulase at 0.2% by weight of raspberry leaf powder to the water, and enzymolyze it at 30°C for 10 minutes to obtain enzymatic hydrolysis After the raspberry leaf powder.

[0058] c) Ethanol extraction: filter the raspberry leaf powder after enzymolysis, wash and drain, add 75% (v / v) aqueous ...

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Abstract

The invention provides a raspberry leaf alcohol extract biopesticide and a preparation method thereof, and belongs to the technical field of biopesticides. The raspberry leaf alcohol extract biopesticide comprises raspberry leaf alcohol extract, Sapindus mulorossi seed oil, sapindus saponin, chitosan and sodium carbonate or sodium bicarbonate. The preparation method comprises the following steps:dissolving the above substances in water according to the formula, and stirring to completely emulsify to obtain a stable emulsion, namely the raspberry leaf alcohol extract biopesticide. The biopesticide has good repelling and killing effects on aphids and locusts, the preparation method is simple, effective components are derived from raspberry leaves, the formula of the biopesticide does not contain components harmful to the environment, and the biopesticide has a considerable control effect on the aphids and the locusts, and is of great significance for environmental protection.

Description

Technical field: [0001] The invention belongs to the technical field of biological pesticides, and relates to a biological pesticide of raspberry leaf ethanol extract and a preparation method thereof. Background technique: [0002] Raspberry (scientific name: Rubusidaeus L. ) is a woody plant of the genus Rubus in the family Rosaceae. It is a fruit with a sweet and sour taste and barb thorns on the branches of the plant. Raspberry has many aliases, such as: raspberry, raspberry, raspberry, raspberry, raspberry, wild berry, raspberry, black currant. [0003] The fruit of raspberry is a kind of aggregate fruit, which is red, golden and black. It is used as a fruit in Europe and the United States. It is widely distributed in China but is little known. It is only cultivated in a small amount in Northeast China and is relatively rare in the market. The raspberry plant can be used as medicine and has a variety of medicinal values. Its fruit has the effect of tonifying the kidney...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/34A01N65/08A01N43/16A01N59/00A01P7/04
CPCA01N43/16A01N59/00A01N65/00A01N65/08A01N65/34
Inventor 王云生刘学诗郭芙蓉汪琪胡绍德江滕
Owner ANHUI AGRICULTURAL UNIVERSITY
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