Nucleic acid detection module, detection unit and detection system

Abstract

The invention discloses a nucleic acid detection module, detection unit and detection system. The nucleic acid detection module comprises a pretreatment assembly and a micro-fluidic detection assembly. The pretreatment assembly comprises a nucleic acid extraction element and a main body module. The main body module comprises a washing cavity and a reaction cavity which are matched with the nucleicacid extraction element. The washing cavity is used for accommodating a washing reagent, and the washing reagent is used for purifying nucleic acid. The reaction cavity is used for accommodating a nucleic acid amplification reaction system capable of amplifying nucleic acid to form amplicons. The micro-fluidic detection assembly comprises a micro-fluidic channel, the micro-fluidic channel is connected with the reaction cavity, the micro-fluidic channel is used for accommodating a detection reaction system containing amplicons and an auxiliary detection reagent, and the detection reaction system can generate a detectable signal. The pretreatment assembly and the micro-fluidic assembly are combined, the extracted nucleic acid is amplified by adopting an iRPAS signal amplification method, and heating is carried out by a smart phone in the amplification process, so that sample collection, nucleic acid release, purification, amplification and detection are completely integrated.

Description

technical field [0001] The invention particularly relates to a nucleic acid detection module, a detection unit and a detection system, belonging to the technical field of microfluidic detection. Background technique [0002] Nucleic acid detection is of great significance in life sciences and is widely used in clinical diagnosis, agricultural monitoring, food safety and detection of plant pathogen infection. In addition, with the in-depth understanding of human health genome information, various types of nucleotide mutations such as gene replacement, insertion and deletion have been proven to be closely related to diseases, such as genetic diseases, metabolic diseases and cancer, etc., even single nucleotide Acid polymorphisms (SNPs) also have important relevance for individual disease susceptibility. Therefore, there is an urgent need for a nucleic acid detection method with high sensitivity and single base specificity. Traditional nucleic acid detection methods such as D...

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Problems solved by technology

However, this method still requires an external heating device to provide the heat required for the reaction (60-65°C), and the detection specificity does not achieve a single base difference

The entire detection device is on a macroscopic millimeter scale, which cannot realize microfluidic chips; and it is mainly based on syringes and supporting devices to purify and detect nucleic acids on a macroscale. The loop-mediated isothermal amplification method is also used, and the reaction needs to be carried out at 60- Performed at 65°C, additional heating equipment is required for assistance, and the specificity of this method cannot reach the level of single base difference

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