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A strain of Trichoderma harzianum mu153 and its application

A technology of Phytophthora infestans and fungal agent, which is applied in the direction of application, chemicals for biological control, biocides, etc., and can solve the problems of low species or yield, poor inhibitory effect of pathogenic bacteria, etc.

Active Publication Date: 2021-08-31
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as one of the most widely used biological agents in agricultural production, Trichoderma wild strains have fewer types or yields of volatile organic compounds and have poor inhibitory effects on pathogenic bacteria

Method used

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  • A strain of Trichoderma harzianum mu153 and its application
  • A strain of Trichoderma harzianum mu153 and its application
  • A strain of Trichoderma harzianum mu153 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1, Obtaining and characteristic detection of African Trichoderma harzianum mutant strain MU153

[0078] In this example, a mutant strain with antibacterial effect on Fusarium oxysporum was obtained by mutagenizing Trichoderma afroharzianum ACCC 33109 with protoplast ultraviolet light.

[0079] 1. Materials

[0080]Species: Trichoderma afroharzianum (Trichoderma afroharzianum) ACCC 33109; Fusarium oxysporum f.sp. cubic race4) ACCC 38875 is Fusarium oxysporum, hereinafter referred to as Fusarium oxysporum ACCC 38875.

[0081] Medium:

[0082] Protoplast regeneration medium: glucose 20g, agar 20g, yeast extract 1g, ammonium sulfate 1.4g, potassium dihydrogen phosphate 2.0g, magnesium sulfate heptahydrate 0.3g, iron sulfate heptahydrate 0.05g, manganese sulfate 0.01g, sulfuric acid heptahydrate Zinc 0.01g, dilute to 1L with 0.6mol / L sodium chloride osmotic pressure stabilizer.

[0083] Synthetic medium: fructose 20g, agar 20g, yeast extract 3g, threonine 3g, dipo...

Embodiment 2

[0125] The mensuration of the bacteriostasis of embodiment 2, African Trichoderma harzianum MU153

[0126] Detect the volatile organic compounds that wild strain Trichoderma harzianum ACCC 33109 and the Trichoderma harzianum MU153 of embodiment 1 produce to cucumber wilt (Fusarium oxysporum), tobacco black shank (Phytophthora infestans) and tobacco red spot disease (Alternaria longiferium) antibacterial effect.

[0127] 1. Strains to be tested

[0128] Trichoderma afroharzianum (Trichoderma afroharzianum) ACCC 33109, hereinafter referred to as ACCC33109;

[0129] Trichoderma harzianum MU153 of Example 1, hereinafter referred to as MU153 for short.

[0130] 2. Pathogens tested

[0131] Fusarium oxysporum f.sp.cucumerinum ACCC 37438, hereinafter referred to as ACCC 37438;

[0132] Alternaria longipes (Alternaria longipes) ACCC 30002, hereinafter referred to as ACCC 30002;

[0133] Phytophthora nicotianae (Phytophthora nicotianae) ACCC 38636 is tobacco black shank fungus, he...

Embodiment 3

[0140] The measurement of African Trichoderma harzianum MU153 of embodiment 3, embodiment 1 to the control effect of cucumber wilt

[0141] Pathogen tested: Fusarium oxysporum f. sp. cucumerinum ACCC 37438, hereinafter referred to as Fusarium oxysporum ACCC 37438.

[0142] Strains to be tested: Trichoderma afroharzianum ACCC 33109, hereinafter referred to as ACCC 33109; Trichoderma afroharzianum MU153 of Example 1, hereinafter referred to as MU153.

[0143] Experimental procedure: Obtain activated wild strain Trichoderma africanus ACCC 33109, mutant strain MU153 and Fusarium oxysporum ACCC 37438 bacterial cakes with a hole punch (Φ=6mm), inoculate them into PDB medium, and use 6 layers of Spores were obtained by filtration with gauze and two layers of lens-cleaning paper, and the concentration of spores was adjusted to 1.0×10 7 conidia / ml spare. The spore suspensions of Trichoderma africanus harzianum ACCC33109 and MU153 were mixed with the spore suspension of Fusarium oxysp...

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Abstract

The invention discloses a African Trichoderma harzianum MU153 and its application. The African Trichoderma harzianum MU153 disclosed in the present invention has a preservation number of CGMCC NO.19036 in the General Microorganism Center of China Microbiological Culture Collection Management Committee. Experiments have proved that Trichoderma harzianum MU153 of the present invention can produce volatile organic compounds with strong antibacterial effect, which are used to inhibit pathogenic bacteria: Fusarium oxysporum, Alternaria and Phytophthora. The Trichoderma harzianum MU153 of the present invention and the bacterial agent prepared by using the strain have great application potential in biological control, and are also important microbial resources for developing natural biological active substances, and have broad application prospects.

Description

technical field [0001] The invention relates to a African Trichoderma harzianum MU153 and its application in the field of biotechnology. Background technique [0002] Fusarium wilt is the main soil-borne plant disease, which can become more serious with the expansion of planting area and the increase of continuous cropping years. Take cucumber fusarium wilt as an example, this disease can occur in any stage of cucumber growth, the incidence rate is 10-30%, and all of them will die in severe cases. There are a variety of microorganisms that can effectively prevent and control Fusarium wilt in the soil in nature, such as Trichoderma spp., Bacillus spp., Streptomycess pp., and the like. Trichoderma has the characteristics of fast growth, strong adaptability and wide antibacterial spectrum, especially for Fusarium spp., Phytophthora spp. and Pythium spp. in the soil. Effect, widely used in pest control. [0003] The biocontrol mechanisms of Trichoderma include nutrient and ni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14C12N1/145C12R2001/885
Inventor 顾金刚陈敬师黄玉洋向杰郭清华李世贵马锐
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI