Gene detection kit for guiding administration of antihypertensive drug verapamil

Abstract

The invention provides a method and kit for guiding administration of verapamil by simultaneously detecting multiple loci related to the metabolism of the antihypertensive drug verapamil through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) because different single nucleotide polymorphism (SNP) loci have extension primers with different molecular weights. The method includes the following steps: designing multiple amplification primers and extension primers according to eight target SNP loci to be detected; preparing a multiple amplification primerreaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single-base extension reactions on the eight target SNP loci by using multiple sets of primers; and performing time-of-flight mass spectrometry analysis on the products after the single-base extension reactions, and identifying the genotypes of different drug metabolism-related SNP loci according to the products of different-molecular-weight extension primers represented by mass spectrum peaks to guide the administration of the antihypertensive drug verapamil. At the same time, the invention provides the detection kit using the method. The method can simultaneously detect the eight SNP loci related to the metabolism of the antihypertensive drug verapamil, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simple and convenient result analysis, and extremely wide application fields.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and relates to a method for detecting multiple PCR single-base extension products by using mass spectrometry characteristic peaks and its products. The method can simultaneously detect multiple PCR single-base extensions in four multiplex PCR reactions Amplified oligonucleotide products. More specifically, the method uses different time-of-flight mass spectrometry characteristic peaks generated by different target oligonucleotide fragments in the process of mass spectrometry typing, and simultaneously detects multiple target SNP sites to guide the antihypertensive drug Vera The use of Pami. Background technique [0002] Human genetic information is stored in the genome. In 2002, the Human Genome Project, an international cooperation project, was finally completed, drawing a fine map of the human genome structure and providing a reference sequence for related research. The human genome...

AI Technical Summary

Problems solved by technology

However, the detection method and kit provided by the invention patent only target the polymorphic site of the adrenergic receptor gene, and the detection objects have limitations

Moreover, this method mainly uses the detection method of fluorescent quantitative PCR. Since fluorescent quantitative PCR needs to design specific probes for the variation of SNP sites, the throughput of this method is low, and only one polymorphic site can be measured in one experiment. Suitable for multiple SNP detection

In addition, if it is necessary to obtain all relevant SNP information, it is necessary to conduct multiple detection tests, which will increase the cost

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