The invention provides a primer combination for differentiating
verapamil individualized medication types. An extension primer which has different molecular weights at different
single nucleotide polymorphism (SNP) sites is utilized, so that through matrix-assisted
laser desorption ionization time-of-flight
mass spectrometry (MALDI-TOF MS), a plurality of sites pertinent to
metabolism of a hypertension reducing
medicine namely
verapamil can be detected at the same time, and finally the primer combination for differentiating
verapamil individualized medication types is obtained. A preparation method of the primer combination comprises the steps of according to 8 objective SNP sites to be detected, respectively designing a
multiplex amplification primer and an extension primer; compounding amultiplex amplification primer
reaction system and an extension primer
reaction system; in the reaction systems, performing amplification and a single-base extension reaction on the 8 objective SNP sites at the same time with multiple sets of primers; and performing time-of-flight
mass spectrometry analysis on products after the single-base extension reaction, identifying the genotypes of different
drug metabolism pertinent SNPs according to products of different molecular weight extension primers represented by
mass spectrum peaks, and guiding the medication of the hypertension reducing
medicine namely the verapamil. The primer combination disclosed by the invention can detect 8
metabolism pertinent SNP sites of the hypertension reducing
medicine namely the verapamil at the same time, and has the advantages of being low in cost, free from synthetized probes, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.