Gene detection kit for medication guidance of antihypertensive drug lacidipine

Abstract

The invention provides a method utilizing extension primers with different molecular weights at different SNP (single nucleotide polymorphism) sites to detect multiple sites related to metabolism of an antihypertensive drug lacidipine by MALDI-TOF-MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) and guide medication of lacidipine finally as well as a kit. The method comprises steps as follows: designing multiple amplification primers and extension primers respectively according to six target SNP sites to be detected; preparing a multi-amplification primer reaction system and an extension reaction system; performing amplification and single-base extension reactions on the six target SNP sites by multiple primers simultaneously respectively; performing time-of-flight mass spectrometry on products obtained after the single-base extension reaction, identifying genetypes of SNPs related to different drug metabolisms according to products, represented by massspectrum peaks, of extension primers with different molecular weights, and guiding the medication of the antihypertensive drug lacidipine. Meanwhile, the invention provides a detection kit utilizingthe method. The SNP sites related to metabolism of six antihypertensive drug lacidipine can be detected simultaneously, and the method has the advantages of low cost, no need of probe synthesis, low time consumption, simple and convenient result analysis and quite wide application field.

Description

Technical field [0001] The invention belongs to the field of molecular biology detection, and relates to a method and product for detecting multiple PCR single-base extension products using mass spectrometry characteristic peak maps. The method can simultaneously detect multiple PCR single-base extensions in two multiple PCR reactions Amplified oligonucleotide product. More specifically, this method uses different time-of-flight mass spectrometry characteristic peaks generated by different target oligonucleotide fragments during mass spectrometry typing to detect multiple target SNP sites at the same time to guide the antihypertensive drug Rasi Medication of Diping. Background technique [0002] Human genetic information is stored in the genome. In 2002, the final completion of the Human Genome Project, an international cooperation project, produced a detailed map of the human genome structure and provided reference sequences for related research. The human genome sequence cons...

AI Technical Summary

Problems solved by technology

However, the detection method and kit provided by this invention patent only target the polymorphic site of the adrenergic receptor gene, and the detection objects have limitations.

Moreover, this method mainly uses the detection method of fluorescent quantitative PCR. Since fluorescent quantitative PCR needs to design specific probes for the variation of SNP sites, the throughput of this method is low, and only one polymorphic site can be measured in one experiment. Suitable for detection of multiple SNPs

In addition, if it is necessary to obtain the information of all relevant SNPs, it is necessary to conduct multiple detection tests, which will increase the cost

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