Gene detection kit for guiding individualized medication of nitrendipine drug for reducing high blood pressure

Abstract

The invention provides a method and kit for guiding medication of nitrendipine. The method comprises steps as follows: multiplex amplification primers and an extension primer are designed respectivelyaccording to two to-be-detected target SNP sites; a multiplex amplification primer reaction system and an extension primer reaction system are prepared; in the reaction systems, an amplification reaction and a single base extension reaction are performed on the two target SNP sites respectively by using multiple sets of primers; products obtained by the single base extension reaction are subjected to time-of-flight mass spectrometry analysis, according to extension primer products with different molecular weights and represented by mass spectrum peaks, genotypes of different SNPs related to drug metabolism are identified, and medication of the nitrendipine drug for reducing high blood pressure is guided. Besides, the invention also provides a detection kit using the method. The kit can detect two SNP sites related to metabolism of the nitrendipine drug for reducing high blood pressure simultaneously and has the advantages that the kit is low in cost, doesn't need synthesis probe, takes short time and is simple and convenient in result analysis and very broad in application field.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and relates to a method for detecting multiple PCR single-base extension products by using mass spectrometry characteristic peaks and its products. The method can simultaneously detect multiple PCR single-base extension products in a multiplex PCR reaction. Increased oligonucleotide products. More specifically, the method uses different time-of-flight mass spectrometry characteristic peaks generated by different purpose oligonucleotide fragments in the process of mass spectrometry typing, and simultaneously detects multiple target SNP sites to guide the antihypertensive drug nitrene. Dipine medication. Background technique [0002] Human genetic information is stored in the genome. In 2002, the Human Genome Project, an international cooperation project, was finally completed, drawing a fine map of the human genome structure and providing a reference sequence for related research. The ...

AI Technical Summary

Problems solved by technology

However, the detection method and kit provided by the invention patent only target the polymorphic site of the adrenergic receptor gene, and the detection objects have limitations

Moreover, this method mainly uses the detection method of fluorescent quantitative PCR. Since fluorescent quantitative PCR needs to design specific probes for the variation of SNP sites, the throughput of this method is low, and only one polymorphic site can be measured in one experiment. Suitable for multiple SNP detection

In addition, if it is necessary to obtain all relevant SNP information, it is necessary to conduct multiple detection tests, which will increase the cost

Images