Grosvenorine-metal zinc complex and preparation method thereof

A mogrosiderin and complex technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of limited bioavailability, low metal copper complexation rate, etc., and achieve expansion and modification. Scope and application range, high complexation rate, high operability effect

Active Publication Date: 2020-06-05
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The flavonoids of Luo Han Guo reported in this literature are kaempferol-3,7-α-L-dirhamnoside, which has a low complexation rate with metal copper, and the bioavailability is also limited, and the biological activity needs to be further improved. improve

Method used

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  • Grosvenorine-metal zinc complex and preparation method thereof
  • Grosvenorine-metal zinc complex and preparation method thereof
  • Grosvenorine-metal zinc complex and preparation method thereof

Examples

Experimental program
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Effect test

preparation example 1

[0080] The preparation of Mogrosin:

[0081] 1. Extraction: Place 50kg of broken-shelled fresh Luo Han Guo in 90°C, a total of 200kg of hot water, extract twice for 2 hours each time, pass through a 100-mesh silk bag to obtain the extract.

[0082] 2. Pass through ultrafiltration membrane: pass the obtained leaching solution through a ceramic membrane with a molecular weight cut-off of 2000, and collect the permeate.

[0083] 3. Filtering nanofiltration membrane: pass the permeate through a nanofiltration membrane with a molecular weight cut-off of 300Da, and collect the retentate.

[0084] 4. Concentration: Concentrate the obtained retentate to 45 brix through a single-effect concentrator.

[0085] 5. Crystallization: put the concentrated solution in 6 times the weight of 60% acetic acid aqueous solution, stir evenly, place it at 4° C. for 24 hours, and then filter to obtain crystals.

[0086] 6. Chromatographic separation: Dissolve 150g of crystals in 15% ethanol aqueous s...

preparation example 2

[0090] 1. Extraction: Place 50kg of broken-shelled fresh Luo Han Guo in a total of 300kg of hot water at 60°C, extract 3 times for 2 hours each time, pass through a 160-mesh silk bag to obtain the extract.

[0091]2. Pass through ultrafiltration membrane: pass the obtained extract through a ceramic membrane with a molecular weight cut-off of 3000, and collect the permeate.

[0092] 3. Filtering through nanofiltration membrane: pass the permeate through a nanofiltration membrane with a molecular weight cut-off of 400Da, and collect the retentate.

[0093] 4. Concentration: Concentrate the obtained retentate to 53brix through a single-effect concentrator.

[0094] 5. Crystallization: put the concentrated solution in 8 times the weight of 70% acetic acid aqueous solution, stir evenly, place it at 10°C for 30 hours, and then filter to obtain crystals.

[0095] 6. Chromatographic separation: Dissolve 150g of crystals in 25% ethanol aqueous solution, put it on the preparative chrom...

preparation example 3

[0099] 1. Extraction: Place 50kg of broken-shelled fresh Luo Han Guo in 400kg of hot water at 50°C, extract 24 times, each time for 2 hours, pass through a 200-mesh silk bag to obtain the extract.

[0100] 2. Pass the ultrafiltration membrane: pass the obtained extract solution through a ceramic membrane with a molecular weight cut-off of 4000, and collect the permeate.

[0101] 3. Filtering nanofiltration membrane: pass the permeate through a nanofiltration membrane with a molecular weight cut-off of 500 Da, and collect the retentate.

[0102] 4. Concentration: Concentrate the obtained retentate to 50 brix through a single-effect concentrator.

[0103] 5. Crystallization: put the concentrated solution in 4 times the weight of 75% acetic acid aqueous solution, stir evenly, place it at 420° C. for 24 hours, and then filter to obtain crystals.

[0104] 6. Chromatographic separation: Dissolve 150g of crystals in 10% ethanol aqueous solution, put it on the preparative chromatogra...

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Abstract

The invention provides a grosvenorine-zinc complex and a preparation method thereof. The grosvenorine-zinc complex is a complex of grosvenorine and Zn(II) ions and has a structure represented by a formula (I). The preparation method comprises the following steps: dissolving grosvenorine and salt of metal zinc into water, regulating a pH value by virtue of alkali, carrying out constant-temperaturestirring, and respectively carrying out nanofiltration, desalination, membrane concentration, crystallization, washing and drying, so as to obtain the grosvenorine-zinc complex. The metal complex is formed by the grosvenorine and metal microelement zinc beneficial to a human body, so that the modification range and application range of the grosvenorine are expanded. The complexing rate of the obtained metal zinc complex is over 84%, the biological utilization rate is high, and the metal zinc complex has excellent anti-tumor biological activity and particularly has selective inhibitory activities to melanoma cells and hepatoma cells.

Description

technical field [0001] The invention relates to a preparation method of a metal complex, in particular to a mogroside flavin metal zinc complex and a preparation method thereof. Background technique [0002] Coordination compounds are a kind of compounds that have ligands and metal atoms combined by coordination bonds, and are formed according to a certain composition and spatial configuration. Rosenberg and others in the United States found that cisplatin has inhibitory effects on testicular cancer, ovarian cancer, bladder cancer, breast cancer, malignant lymphoma and leukemia, showing broad-spectrum anti-cancer activity. This work created the entry of inorganic metal complexes into anti-tumor The first of its kind in the ranks of drugs, laid the foundation for bioinorganic medicinal chemistry. Later, people gradually realized that metal complexes play a very important role in the life process and are an important direction of new drug research. The purpose of in-depth res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H23/00C07H1/00C07H17/07C07H1/08
CPCC07H1/00C07H1/08C07H17/07C07H23/00
Inventor 何安乐熊瑶黄华学
Owner HUNAN HUACHENG BIOTECH
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