Pseudomonas aeruginosa biological membrane and preparation method and application thereof

Abstract

The invention belongs to the technical field of macromolecules, and discloses a pseudomonas aeruginosa biological membrane and a preparation method and application thereof. The method comprises the following steps: inoculating bacteria onto an agar plate, selecting a single colony, inoculating the single colony into 5mL of LB culture solution, performing shaking to centrifuge a bacterial solution,discarding supernatant, washing bacterial precipitate with sterile normal saline, adjusting bacterial suspension with normal saline, and keeping obtained bacterial solution for later use; and adding10ml of the prepared experimental bacterial solution into a 50ml conical flask, adding 10 carriers, and carrying out shaking culture at constant temperature of 37 DEG C at a speed of 110rpm for 20 hours to adhere bacteria. According to the structure of the pseudomonas aeruginosa biological membrane cultured in vitro, the carriers are put into the bacterial suspension with adjusted concentration toculture the biological membrane structure, and then the bacterial concentration is detected and adjusted. The bacterial biological membrane is stable in structure and good in repeatability. The prepared carriers are transplanted into the abdominal cavity of a mouse to cause pseudomonas aeruginosa biological membrane infection, and then the pathogenic mechanism and mild moxibustion intervention treatment effect of the biological membrane are studied.

Description

technical field [0001] The invention belongs to the technical field of macromolecules, and in particular relates to a biofilm of Pseudomonas aeruginosa and its preparation method and application. Background technique [0002] Currently, the commonly used prior art in the industry is as follows: Bacterial biofilm (BF)-associated infection is a widespread and multidisciplinary disease. BF refers to the secretion of polysaccharide-protein complexes such as polysaccharide matrix, fibrin, and lipoprotein after bacteria are adsorbed on the surface of solid substances (such as medical organic materials or the surface of body mucosa), so that the bacteria adhere to each other and wrap their own cells in the biological environment. In the capsule, a dense film-like state is formed, which is a three-dimensional aggregate of bacteria. With the widespread use of biomedical materials, clinical infections related to bacterial biofilms are also increasing. According to statistics, 65% of ...

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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: Pseudomonas aeruginosa biofilm infection of Western medicine antibiotic resistance phenomenon, and the treatment of traditional Chinese medicine has a certain curative effect, but it is difficult to completely cure, and the economic burden is large, and it is still difficult to cure. May cause flora imbalance, liver and kidney damage, etc.

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