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A maarf1 transcription factor interacting with the promoter of the key enzyme gene in the banana salicylic acid synthesis pathway and its application

A technology of transcription factors and synthases, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of loss of resistance and weakened basic resistance of mutant plants, and achieve the effect of solving the bottleneck problem.

Active Publication Date: 2021-07-02
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Application Information

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Problems solved by technology

In the sard1-1 / cbp60g-1 double mutant, the induction of ICS1 gene expression level and salicylic acid level in vivo by pathogenic bacteria was inhibited, the basal resistance of mutant plants was weakened, and systemic acquired resistance was lost

Method used

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  • A maarf1 transcription factor interacting with the promoter of the key enzyme gene in the banana salicylic acid synthesis pathway and its application
  • A maarf1 transcription factor interacting with the promoter of the key enzyme gene in the banana salicylic acid synthesis pathway and its application
  • A maarf1 transcription factor interacting with the promoter of the key enzyme gene in the banana salicylic acid synthesis pathway and its application

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Embodiment Construction

[0030] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0031] 1. Banana isochoristate synthase MaICS promoter driving salicylic acid synthesis

[0032] The young roll leaves of the cultivar Brazilian banana were selected as materials, and the genomic DNA was extracted using the polysaccharide and polyphenol plant genomic DNA extraction kit (DP360) of Beijing Tiangen Biochemical Technology Co., Ltd., and the DNA quality was detected by 1% agarose gel electrophoresis. With the above-mentioned banana...

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Abstract

The present invention provides a transcription factor MaARF1, which is a protein encoded by a gene whose nucleotide sequence is shown in SEQ ID NO:2. The invention also provides the coding gene and application of the transcription factor MaARF1. The present invention uses the yeast one-hybrid method to obtain the MaARF1 transcription factor that interacts with the promoter of the key enzyme gene in the banana salicylic acid synthesis pathway. The transcription factor MaARF1 is a regulatory factor upstream of the MaICS gene and has a certain effect on the biosynthesis of banana salicylic acid. It plays an important regulatory role, and its application is conducive to the use of transgenic technology to breed new varieties of bananas resistant to Fusarium wilt, and has positive significance for solving the bottleneck problem of sustainable development of the banana industry.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a MaARF1 transcription factor interacting with a promoter of a key enzyme gene in a banana salicylic acid synthesis pathway and an application thereof. Background technique [0002] Bananas and plantains are one of the important crops in tropical and subtropical developing countries, the second largest fruit in the world after citrus, and one of the fruits with the largest fresh fruit consumption in the world. According to FAO statistics, in 2013, the world's total output of fragrant (big) bananas was 145 million tons; my country's banana planting area was 5.9567 million mu, and the output reached 12.1146 million tons, accounting for 8.35% of the world's total output; it is the second largest banana production after India country. The banana industry has become a pillar industry for farmers in these areas to get rid of poverty and promote local econom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/113C12N15/82A01H5/00A01H6/00C12N15/11
CPCC07K14/415C12N9/90C12N15/8235C12N15/8243C12N15/8282C12Y504/04002
Inventor 王卓王静毅贾彩红刘菊华徐碧玉金志强张建斌苗红霞
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI