MaARF1 transcription factor interacting with promoter of key enzyme gene in banana salicylic acid synthetic route and application of MaARF1 transcription factor
A technology of transcription factor and synthetase, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as loss of resistance, weakening of basic resistance of mutant plants, etc., and achieve the effect of solving bottleneck problems
Active Publication Date: 2020-06-12
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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In the sard1-1 / cbp60g-1 double mutant, the induction of ICS1 gene expression level and salicylic acid level in vivo by pathogenic bacteria was inhibited, the basal resistance of mutant plants was weakened, and systemic acquired resistance was lost
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[0030] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.
[0031] 1. Banana isochoristate synthase MaICS promoter driving salicylic acid synthesis
[0032] The young and tender roll leaves of the cultivar Brazilian banana were selected as materials, and the genomic DNA was extracted using the polysaccharide and polyphenol plant genomic DNA extraction kit (DP360) of Beijing Tiangen Biochemical Technology Co., Ltd., and the DNA quality was detected by 1% agarose gel electrophoresis. With the above-menti...
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The invention provides a transcription factor MaARF1. The transcription factor MaARF1 is a protein coded by a gene with a nucleotide sequence as shown in SEQ ID NO: 2. The invention also provides an encoding gene and application of the transcription factor MaARF1. The MaARF1 transcription factor interacting with a promoter of a key enzyme gene in a banana salicylic acid synthetic route is obtainedby utilizing a yeast one-hybrid method; the transcription factor MaARF1 is a regulatory factor at the upstream of the MaICS gene, has an important regulation effect on banana salicylic acid biosynthesis, is beneficial to cultivation of a new banana fusarium wilt-resistant variety by using a transgenic technology, and is of positive significance for solving the bottleneck problem of sustainable development of the banana industry.
Description
technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a MaARF1 transcription factor interacting with a promoter of a key enzyme gene in a banana salicylic acid synthesis pathway and an application thereof. Background technique [0002] Bananas and plantains are one of the important crops in tropical and subtropical developing countries, the second largest fruit in the world after citrus, and one of the fruits with the largest fresh fruit consumption in the world. According to FAO statistics, in 2013, the world's total output of fragrant (big) bananas was 145 million tons; my country's banana planting area was 5,956,700 mu, and the output reached 12,114,600 tons, accounting for 8.35% of the world's total output; it is the second largest banana production after India country. my country's banana production areas are mainly distributed in Guangdong, Guangxi, Hainan, Fujian, Yunnan (excluding Taiwan) and oth...
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IPC IPC(8): C07K14/415C12N15/29C12N15/113C12N15/82A01H5/08A01H6/00C12N15/11
CPCC07K14/415C12N9/90C12N15/8235C12N15/8243C12N15/8282C12Y504/04002
Inventor 王卓王静毅贾彩红刘菊华徐碧玉金志强张建斌苗红霞
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI