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Method for infecting freshwater crabs from paragonimus in laboratories

A laboratory and river crab technology, applied in the field of paragonimus infection of river crabs, can solve problems such as unsatisfactory experimental needs, decreased infection rate, reduction of second intermediate host river crabs, etc.

Inactive Publication Date: 2020-06-19
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, research on paragonimus zoonoses, development of animal models of paragonimus liver fibrosis and pulmonary fibrosis, research on proteins secreted by paragonimus adults, and conservation of special species of paragonimus all require a large number of infections. For the river crabs of Paragonimus, with the reduction of forests, the population of wild animal hosts is gradually shrinking; the extensive use of pesticides and fertilizers, the improvement of human medical and health conditions, and the reduction of the incidence of Paragonimus directly lead to the second intermediate The reduction of the host crab, and the infection rate of Paragonimus in the river crab has shown a downward trend year by year (the natural infection rate is about 20%), which cannot meet the needs of the experiment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Collect the feces of rats infected with Paragonimus flukes in the laboratory for more than 100 days, and add 180ml of water to melt each 20g of feces, then gently shake and shake, and pass through No. 5 medicine sieve (180μm±7.6μm) to fully filter the feces Granules, let the eggs pass through the No. 5 drug sieve smoothly, and the liquid containing the eggs through the No. 5 drug sieve passes through the No. 9 drug sieve (75±4.1um) to ensure that the eggs of Paragonimus flukes are attached to the No. 9 drug sieve , the 9 drug sieve with the eggs attached should be sealed in a fresh-keeping bag and stored in a closed light environment (16-20°C) for later use, and the time should not exceed 24 hours.

[0017] 2. Paragonimus cercariae infection of snails

[0018] 2.1 Establish a snail breeding pool (made of 1mm stainless steel plate) with a length of 100×width 60×height 30cm and a water depth of 20cm;

[0019] 2.2 Put 80 snails in each pool, and keep 20 snails on the w...

Embodiment 2

[0024] 1. Collect the feces of rats infected with Paragonimus flukes in the laboratory for more than 100 days, and add 180ml of water to melt each 20g of feces, then gently shake and shake, and pass through No. 5 medicine sieve (180μm±7.6μm) to fully filter the feces Granules, let the eggs pass through the No. 5 drug sieve smoothly, and the liquid containing the eggs through the No. 5 drug sieve passes through the No. 9 drug sieve (75±4.1um) to ensure that the eggs of Paragonimus flukes are attached to the No. 9 drug sieve , the 9 drug sieve with the eggs attached should be sealed in a fresh-keeping bag and stored in a closed light environment (16-20°C) for later use, and the time should not exceed 24 hours.

[0025] 2. Paragonimus cercariae infection of snails

[0026] 2.1 Establish a snail breeding pool (made of 1mm stainless steel plate) with a length of 100×width 60×height 30cm and a water depth of 20cm;

[0027] 2.2 Put 60 snails in each pool, and keep 15 water cabbages ...

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PUM

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Abstract

The invention discloses a method for infecting freshwater crabs from paragonimus in laboratory conditions. The method mainly comprises the steps of collecting excrement of rats, infected with paragonimus, with eggs after microscopic examination, melting the excrement with clear water, sieving the molten excrement with the eggs through a No.5 medicine sieve, sieving the excrement with a No.9 medicine sieve, cleaning the No.9 medicine sieve attached with the eggs in a paragonimiasis feeding pool so that the eggs fully enter the paragonimiasis feeding pool, wherein 60-80 pieces of paragonimiasisare put into each pool, the water temperature of the paragonimiasis breeding pool is kept at 18-23 DEG C, and after the eggs enter the paragonimiasis breeding pool for two weeks, the paragonimiasis isrepelled once every day; transferring the paragonimiasis infected with paragonimus for more than three weeks into a water tank of a freshwater crab feeding and breeding box, wherein 80-100 freshwatercrabs with the weight of 20-30 g are put into each feeding and breeding box. Along with escape of ripe cercariae of the paragonimiasis, after symbiosis of the paragonimiasis and the freshwater crabsin the feeding and breeding box for six weeks, the infection rate of paragonimus of the freshwater crabs can reach 90% or above and far exceeds the current natural infection rate, and the requirementof laboratory research on infection of paragonimus of the freshwater crabs can be completely met.

Description

technical field [0001] The invention relates to a method for infecting paragonimus in river crabs under laboratory conditions. Background technique [0002] At present, research on paragonimus zoonoses, development of animal models of paragonimus liver fibrosis and pulmonary fibrosis, research on proteins secreted by paragonimus adult worms, and conservation of special species of paragonimus require a large number of infections. For the river crabs of Paragonimus, with the reduction of forests, the population of wild animals that protect the insects is gradually shrinking; the extensive use of pesticides and fertilizers, the improvement of human medical and health conditions, and the reduction of the incidence of Paragonimus directly lead to the second intermediate The reduction of the host crab, and the infection rate of Paragonimus in the river crab showed a downward trend year by year (the natural infection rate was about 20%), which could not meet the needs of the experi...

Claims

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Application Information

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IPC IPC(8): A01K61/59A01K61/51A01K67/033
CPCA01K67/033A01K61/51A01K61/59Y02A40/81
Inventor 王文林郑红角建林李波
Owner KUNMING MEDICAL UNIVERSITY
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