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A kind of microbial agent m1 for preventing and treating corn stalk rot and its application

A technology of corn stalk rot and microbial agent, applied in the field of microorganisms, to achieve the effects of increasing emergence rate, plant height, and effective ear number

Active Publication Date: 2021-04-20
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few microbial inoculants that can be used for the prevention and control of corn diseases at present, and it is necessary to develop microbial inoculants suitable for the control of corn stalk rot

Method used

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  • A kind of microbial agent m1 for preventing and treating corn stalk rot and its application
  • A kind of microbial agent m1 for preventing and treating corn stalk rot and its application
  • A kind of microbial agent m1 for preventing and treating corn stalk rot and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The separation of three kinds of bacterial strains of embodiment 1

[0029] (1) Isolation of Agrobacterium

[0030] Isolation of Agrobacillus: Add 10g of potato field soil sample into 90mL of sterile water, shake (180rpm / min) at room temperature for about 30min to make a turbid solution, and then dilute the sample with sterile water. The diluted 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 0.1 mL of each concentration sample was coated on the broth culture medium (NA), and the coated plate was inverted and cultured in a 37°C incubator. After 2 days, a single colony with different phenotype characteristics was picked from the plate, and the gradient was drawn. The pure strain was obtained by line isolation, and a single colony of the purified strain was picked, transferred to 40% glycerol, and stored in a -80°C ultra-low temperature refrigerator for later use.

[0031] Broth medium (NA): peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, pH 7.3±0.1, sterili...

Embodiment 2

[0052] The preparation method of embodiment two microbial bacterial agents

[0053] ① Compatibility between strains

[0054] Referring to the determination method of Barbosa, one functional strain was streaked on half of the LB solid medium plate, and another functional strain was vertically streaked on the other half, and each treatment was repeated 3 times. Place the plate in a biochemical incubator at 37°C for cultivation, and observe and record the compatibility reaction between the two strains after 24 hours. The two strains that do not produce antibacterial bands are compatible, otherwise they are incompatible. Compatibility results show that the three strains do not inhibit each other and can be used in combination.

[0055] ② Preparation of Bacillus fermentation broth

[0056] Single colonies of strains HR15, HR37 and HR55 activated for 24 hours were respectively inoculated into the seed fermentation liquid containing liquid NA medium, and cultured with shaking at 1...

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Abstract

The invention discloses a microbial agent M1 for preventing and treating corn stalk rot and application thereof, including Bacillus subtilis HR15, Bacillus atrophaeus HR37 and Bacillus velezensis HR55 Three strains; the three strains were all preserved in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), and the preservation date was March 6, 2020; among them, the preservation number of Bacillus subtilis HR15 was CGMCC NO.19453; The preservation number of Bacillus HR37 is CGMCC NO.19451; the preservation number of Bacillus Velez HR55 is CGMCC NO.19452. The microbial agent M1 of the present invention has a good control effect on corn stalk rot, can effectively recover the yield loss caused by stalk rot, improve soil nutritional status, and affect the diversity and structure of fungal and bacterial communities in the soil.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial agent M1 used for the prevention and treatment of corn stalk rot and its application. Background technique [0002] Corn is an important food crop in the world, and its planting scale ranks only after wheat. With the change of global climate conditions, continuous cropping, high-density cultivation, etc., the ecological environment of cornfields has changed accordingly, the structure of the dominant flora of pathogenic bacteria in the soil has changed, the accumulation of diseased residues has increased, and the structure of beneficial microbial communities and quantities have also changed significantly. These factors lead to the imbalance of soil microbial community structure in cornfields, and the occurrence of corn stalk rot is becoming more and more serious. [0003] Maize stalk rot is a typical soil-borne disease caused by complex infection of various pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/60C05G3/80C05G5/27A01N63/22A01P1/00A01P3/00C12R1/125C12R1/07
CPCC05D1/02C05G3/00C12N1/20
Inventor 沙月霞沈瑞清朱建祥杨卫东邹金科邢敏马惠兰王喜刚
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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