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A kind of determination method of crop seed vigor

A measuring method and technology for seeds, applied in germination equipment and other directions, can solve problems such as difficulty in simulating the field environment, and achieve the effects of simple detection process, shortened detection time, and accurate and reliable results.

Active Publication Date: 2021-12-17
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the elongated radicle counting method is carried out in an ideal and stable indoor environment, and it is difficult to simulate the complex and changeable field environment, and the results sometimes cannot better reflect the level of seed vigor

Method used

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  • A kind of determination method of crop seed vigor
  • A kind of determination method of crop seed vigor
  • A kind of determination method of crop seed vigor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Plant material

[0030] Rice (Oryza sativa L.) 'Yliangyou 689', 'Zhuliangyou 101', 'Chunyou 84', 'Zheyou 18', 'Zhejing 99', 'Yongxian 15' were used as experiments Materials, each rice variety has two seed samples with high and low vigor gradients, a total of 12 rice seed samples.

[0031] 2. High temperature sealed water flooding treatment + elongated radicle count test

[0032] High-temperature sealed water flooding treatment: After the rice seeds are sterilized by 0.1% sodium hypochlorite solution for 15 minutes, they are rinsed with ultrapure water for 2 to 4 times, each time for 2 minutes, and the water attached to the surface of the rice seeds is blotted with filter paper. Put 15g of rice seeds in a 200ml centrifuge tube, add 150ml of pure water; then place the centrifuge tubes in constant temperature water baths at 35°C, 40°C, 45°C, and 50°C, and let them stand in the dark for 12 hours. Stress treatments were performed, each temperature setting sealed and ope...

Embodiment 2

[0067] 1. Plant material

[0068] Four sunflower varieties of sunflower (Helianthus annuus L.) 'S150', 'S154', 'S167' and 'S189' were used as test materials. Each sunflower variety had 3 seed samples with vigor gradients, and a total of 12 sunflower seed samples.

[0069] 2. High temperature sealed water flooding treatment + elongated radicle count test

[0070] High-temperature sealed water flooding treatment: sunflower seeds are sterilized with 0.1% sodium hypochlorite solution for 15 minutes, rinsed with ultrapure water for 2 to 4 times, each time for 2 minutes, and dry the water attached to the surface of sunflower seeds with filter paper. Put 15g of sunflower seeds in a 200ml centrifuge tube, add 150ml of pure water; then place the centrifuge tubes in constant temperature water baths at 35°C, 40°C, 45°C, and 50°C, and let them stand in the dark for 12 hours. Stress treatments were performed, each temperature setting sealed and open treatments.

[0071] Radical elongatio...

Embodiment 3

[0105] 1. Plant material

[0106] Sweet corn (Zea mays L.) five sweet corn varieties 'Xiantian 5', 'Tianyu 2', 'Meiyu 7', 'Zhongnongtian 488' and 'Huazhen' were used as experimental materials. There are 2 seed samples of high and low vigor gradients for corn varieties, a total of 10 sweet corn seed samples.

[0107] 2. High temperature sealed water flooding treatment + elongated radicle count test

[0108] High-temperature sealed water flooding treatment: After the sweet corn seeds are sterilized by 0.1% sodium hypochlorite solution for 15 minutes, they are washed 2 to 4 times with ultrapure water for 2 minutes each time, and the water attached to the surface of the sunflower seeds is blotted with filter paper. Put 15g of sunflower seeds in a 200ml centrifuge tube, add 150ml of pure water; then place the centrifuge tubes in constant temperature water baths at 35°C, 40°C, 45°C, and 50°C, and let them stand in the dark for 12 hours. Stress treatments were performed, each tempe...

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Abstract

The invention discloses a method for measuring the vigor of crop seeds. The method comprises: after the seeds of the crops to be tested are sterilized, they are placed in pure water at 45°C and left to stand in the dark for 12 hours, and the stress treatment is carried out to obtain the stress treatment. Crop seeds: Germination test is carried out to the crop seeds after the stress treatment, the number of elongated radicles of the crop seeds after the germination test is counted, the percentage of elongated radicles of the seeds is calculated, and the vigor of the crop seeds is measured according to the percentage of elongated radicles. The invention combines the 45°C sealed water flooding treatment with the elongated radicle count after the germination test, effectively simulates the complex and changeable field adversity environment, and is more conducive to reflecting the vitality difference between high-vigorous seed batches, and through correlation The analysis finds that the percentage of elongated radicle of the seed obtained by the method of the present invention has a very significant correlation with the emergence rate of the seed in the field, can accurately predict the situation of the emergence of the seed in the field, and the result is accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of seed detection, in particular to a method for measuring the vigor of crop seeds. Background technique [0002] Seeds are the most basic means of agricultural production, and seed quality is the most important factor for seed safety. Improving seed quality is an important guarantee for high-efficiency, high-quality and high-yield agricultural production. Seed vigor is a general term that determines the potential ability of seeds (batches) to emerge quickly and neatly in the field and grow into normal seedlings, and is an important indicator of seed quality. High-vigorous seeds have obvious growth advantages and production potential, and are of great significance for improving seed storability and field seedling rate, resisting seedling adversity (low temperature, drought, pests and diseases, etc.), saving sowing costs, and increasing crop yield. High-vigorous seeds are an important guarantee for the real...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 黄玉韬曹栋栋陈珊宇梅高甫阮关海吴华平吴玭
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES