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Small and dense low-density lipoprotein cholesterol determination reagent

A low-density lipoprotein and cholesterol technology, applied in the field of small and dense low-density lipoprotein cholesterol assay reagents, can solve the problems of cumbersome operation, bottle opening stability, poor anti-interference ability, low resolution and specificity, and achieve Strong anti-interference ability and good bottle opening stability

Pending Publication Date: 2020-07-10
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method has disadvantages such as cumbersome operation, low resolution and specificity, and long time. Among them, the homogeneous method is relatively simple and fast, but the existing small and dense LDL cholesterol detection kits Poor stability and anti-interference ability

Method used

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  • Small and dense low-density lipoprotein cholesterol determination reagent
  • Small and dense low-density lipoprotein cholesterol determination reagent
  • Small and dense low-density lipoprotein cholesterol determination reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The screening of inorganic salt ion in the first reagent of embodiment 1

[0089] 1. Reagents used

[0090] Note: The manufacturer of the contrast reagent is a manufacturer of products on sale

[0091] Reagent 1:

[0092]

[0093] Reagent 2:

[0094]

[0095] Reagent 3:

[0096]

[0097] Reagent 4

[0098]

[0099]

[0100] Reagent 5

[0101]

[0102] Reagent 6

[0103]

[0104] Reagent 7

[0105]

[0106]

[0107] 2. Detection process

[0108] 1) Testing instrument: automatic biochemical analyzer.

[0109] 2) Samples to be tested: blood collected on an empty stomach, fresh serum, samples cannot be frozen and thawed repeatedly.

[0110] 3) Basic parameters:

[0111] Sample size: 3ul R1 usage: 150ul R2 usage: 50ul

[0112] Main wavelength: 600nm Secondary wavelength: 700nm Methodology: END

[0113] Response direction: + Calibration mode: Y=AX+B

[0114] Incubation time of R1+ sample: 300sec Reaction time after adding R2: 300sec

...

Embodiment 2

[0144] Ratio screening of embodiment 2Trinder color source substance and 4-aminoantipyrine

[0145] 1. Reagents used

[0146] Note: The manufacturer of the comparative reagent is a manufacturer of the product on sale

[0147] Reagent 8

[0148]

[0149] Reagent 9

[0150]

[0151] Reagent 10

[0152]

[0153]

[0154] Reagent 11

[0155]

[0156] Reagent 12

[0157]

[0158] 2. Experimental process

[0159] 1) Purpose of the experiment:

[0160] The different proportions of Trinder color source substance and 4-aminoantipyrine will affect the calibration curve of the reagent, thereby affecting the performance of the reagent. By using the same calibrator and instrument, the Trinder color source substance containing different proportions is tested at the same time Calibrate with 4-aminoantipyrine reagent, and observe the influence of different ratios of Trinder color source substances and 4-aminoantipyrine reagent on calibration.

[0161] 2) Specific exp...

Embodiment 3

[0177] The screening of stabilizer in the first reagent of embodiment 3

[0178] 1. Reagents

[0179] Note: The manufacturer of the contrast reagent is a manufacturer of products on sale

[0180] Reagent 13:

[0181]

[0182]

[0183] Reagent 14:

[0184] Reagent R1: Reagent R2: Azine-N 110mmol / L N-bis(2-ethanesulfonic acid) 110mmol / L Ferrous ion 10mmol / L Peroxidase 2000U / L BSA 10g / L 4-aminoantipyrine 0.82g / L Peroxidase 600U / L Triton 25g / L Cholesterol lipase 800U / L Sodium azide 1.0g / L Cholesterol oxidase 600U / L The solvent is purified water Trinder color source substance 0.24g / L Sodium azide 1.0g / L The solvent is purified water

[0185] Reagent 15:

[0186]

[0187] 2. Experimental process

[0188] 1) Testing instrument: automatic biochemical analyzer.

[0189] 2) Samples to be tested: blood collected on an empty stomach, fresh serum, samples cannot be frozen and thawed repeatedly.

...

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Abstract

The invention provides a small and dense low-density lipoprotein cholesterol detection reagent. The detection reagent comprises a first reagent and a second reagent. Under the combination of inorganicsalt ions, a stabilizer, a buffer solution, peroxidase and the like, the kit is good in bottle opening stability; in addition, the anti-interference capability of the reagent is high, and interference of bilirubin, hemoglobin, chyle and intralipid can be effectively eliminated.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a reagent for measuring small and dense low-density lipoprotein cholesterol. Background technique [0002] Dyslipidemia is an important independent risk factor for atherosclerosis (AS). According to the 2016 revision of the "Guidelines for the Prevention and Treatment of Dyslipidemia in Chinese Adults", the overall prevalence of dyslipidemia in Chinese adults is as high as 40.4%. Hypercholesterolemia in children and adolescents The prevalence rate has increased significantly, suggesting that the burden of dyslipidemia and related diseases in the Chinese population will continue to increase in the future. Early detection of individuals with dyslipidemia, monitoring of changes in blood lipid levels, diagnosis and treatment of hyperlipidemia and dyslipoproteinemia, prevention and risk assessment of atherosclerotic cardiovascular disease (ASCVD) are of great significance....

Claims

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Application Information

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IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 李元丽芮海涛
Owner ZYBIO INC
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