Novel coronavirus nucleic acid preparation and PCR detection integrated device

Abstract

The invention relates to a novel coronavirus nucleic acid preparation and PCR detection integrated device. The device comprises a shell, a sample processing module used for sucking a sample from a sample table, a nucleic acid extraction module used for extracting nucleic acid, a nucleic acid transfer module that comprises a third conveying belt and a nucleic acid extraction gun that extracts the nucleic acid from a nucleic acid extraction plate, and a PCR detection module that comprises a PCR tube, a temperature control bottom plate, a laser emitter, a fluorescence signal detection device, a data processing and control element and a data output panel; and after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser emitter emits laser to irradiate thePCR tube, the fluorescence signal detection device detects a fluorescence signal in the PCR tube, and the data processing and control element receives a detection signal of the fluorescence signal detection device and displays an analysis result on the data output panel.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to an integrated device for nucleic acid preparation and PCR detection of the new coronavirus. Background technique [0002] The statements in this section merely provide background technical information related to the disclosure of the present invention, and do not necessarily constitute prior art. [0003] The new coronavirus (severe acute respiratory syndrome coronavirus 2, 2019-nCoV, severe acute respiratory syndrome coronavirus 2) is particularly contagious, and its nucleic acid detection needs to be carried out in the P3 laboratory, and the operators need to wear protective clothing. Isolation gowns, goggles, etc. enter the P3 laboratory for extraction, and after extraction, the nucleic acid is placed into the PCR system for real-time fluorescent quantitative PCR detection. It is extremely inconvenient and time-consuming to wear protective clothing when extracting...

AI Technical Summary

Problems solved by technology

It is extremely inconvenient and time-consuming to wear protective clothing when extracting nucleic acids. During the operation, the operator is prone to hypoxia and fatigue during long-term operation, which increases the probability of virus infection.

However, real-time quantitative PCR requires a 2-hour process, and requires concentrated samples to be put on the machine together. Scattered samples cannot be made, and they cannot be used for emergency projects. All these make the detection of the new coronavirus not timely and convenient.

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