Method for extracting atractylodes macrocephala polysaccharide by utilizing microbial fermentation method

A microbial fermentation method and atractylodes polysaccharide technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high enzyme cost, long time consumption, low polysaccharide extraction rate, etc., to achieve easy dissolution and promote solution The effect of improving the separation and extraction yield

Active Publication Date: 2020-07-31
深圳立专信息科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Different extraction processes have their own advantages and disadvantages, and the yield of polysaccharides also varies greatly
The hot water extraction method takes a long time and the polysaccharide extraction rate is low; although the enzyme extraction method can obtain a higher polysaccharide yield, the high cost of the enzyme limits its wide application; the ultrasonic extraction method is extracted in hot water. On the basis of adding ultrasonic extraction steps, the extraction yield is relatively high, so it has a relative advantage

Method used

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  • Method for extracting atractylodes macrocephala polysaccharide by utilizing microbial fermentation method
  • Method for extracting atractylodes macrocephala polysaccharide by utilizing microbial fermentation method
  • Method for extracting atractylodes macrocephala polysaccharide by utilizing microbial fermentation method

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Experimental program
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Effect test

Embodiment 1

[0024] Screening and identification of embodiment 1 fermentation strain

[0025] 1. Screening of Rhizopus oryzae CH5 strain

[0026] (1) Add 20g of Zizhi powder crushed through a 40-mesh sieve into a 250-mL Erlenmeyer flask, then add 30mL of sterile water to moisten it, and incubate at 28°C for 4 days. Dilute the enriched culture covered with mold with sterile water 1 × 10 6 After doubling, it was spread on the PDA plate medium, and cultured at 28°C for 3 days, and fungal colonies with different colors and shapes were picked and transferred to fresh PDA medium, and cultured at 28°C for 3 days to obtain 7 purely cultured strains (No. CH1-CH7 respectively), inoculated with fresh PDA medium, and cultured at 28°C for 3 days. Add 5 mL of sterile water to each of the fresh plate cultures of the 7 strains, and stir with an inoculating loop to suspend the spores. The spore liquid was transferred to a sterile test tube, and the spore concentration was adjusted to 2×10 with sterile w...

Embodiment 2

[0043] Embodiment 2 Rhizopus oryzae CH5 is applied to the extraction of Atractylodes macrocephala polysaccharide

[0044] (1) Preparation of Rhizopus oryzae CH5 spore liquid: Plate colonies of Rhizopus oryzae CH5 preserved at 4°C were inoculated on fresh PDA plate medium, cultured at a constant temperature of 28°C for 3 days, added 5 mL of sterile water to the plate culture, and used Agitate the inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spore concentration to 3×10 with sterile water. 8 individual / mL. Described PDA plate medium composition and preparation method are the same as embodiment 2.

[0045] (2) 500-mL triangular flask was sterilized by dry heat at 160°C for 2 hours, added 20 g of Atractylodes macrocephala powder crushed through a 60-mesh sieve, added 50 mL of sterile water, and then inoculated with 2 mL of CH5 spore liquid prepared in step (1), and stirred uniform. The mouth of the triangular flask was ...

Embodiment 3

[0050] Embodiment 3 Rhizopus oryzae CH5 is applied to the extraction of Atractylodes macrocephala polysaccharide:

[0051] (1) Preparation of Rhizopus oryzae CH5 spore liquid: Plate colonies of Rhizopus oryzae CH5 preserved at 4°C were inoculated on fresh PDA plate medium, cultured at a constant temperature of 28°C for 3 days, added 5 mL of sterile water to the plate culture, and used Agitate the inoculation loop to suspend the spores, transfer the spore liquid to a sterile test tube, and adjust the spore concentration to 4×10 with sterile water. 8 individual / mL. Described PDA plate medium composition and preparation method are the same as embodiment 1.

[0052] (2) 2-L triangular flask was sterilized by dry heat at 160°C for 2 hours, added 50 g of Atractylodes macrocephala powder crushed through a 60-mesh sieve, added 150 mL of sterile water, and then inoculated with 5 mL of Rhizopus oryzae CH5 spore liquid prepared in step (1) , stir well. The mouth of the triangular flas...

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Abstract

The invention discloses a method for extracting atractylodes macrocephala polysaccharide by using a microbial fermentation method. The method comprises the following steps: inoculating rhizopus oryzaeGDMCC No:60990 spores into atractylodes macrocephala koidz powder containing sterile water, performing fermenting at 28-30 DEG C for 2-3 days, carrying out ultrasonic water extraction on the fermented product, and then performing concentrating to obtain a concentrate; and carrying out alcohol precipitation and vacuum drying on the concentrate to obtain crude atractylodes macrocephala polysaccharide. Fermentation pretreatment of rhizopus oryzae CH5 is additionally added before ultrasonic water treatment of atractylodes macrocephala polysaccharide. The rhizopus oryzae CH5 grows moderately in the atractylodes macrocephala koidz powder, generate some polysaccharide hydrolase to hydrolyze insoluble polysaccharide structure tissues of the atractylodes macrocephala koidz, and promotes dissociation of combined soluble polysaccharide, so that the polysaccharide can be dissolved out more easily during hot water ultrasonic extraction. Compared with a conventional method without fermentation pretreatment, the extraction yield of the atractylodes macrocephala polysaccharide can be increased by 50% or above.

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of bioengineering, and in particular relates to the application of microbial fermentation technology in the extraction of Atractylodes macrocephala polysaccharide. [0003] (2) Background technology [0004] Atractylodes macrocephalae Rhizoma (Atractylodes macrocephalae Rhizoma), also known as Atractylodes macrocephalae Rhizoma, is the dried rhizome of Atractylodes macrocephala Koidz in the family Compositae. Atractylodes are traditional Chinese medicinal materials for medicines and foods. They are collected in the 2015 Chinese Pharmacopoeia. Its taste, sweetness, warmth; returning spleen and stomach meridians; The effect of the fetus. Indications for spleen deficiency, lack of food, abdominal distension and diarrhea, phlegm retention, dizziness, palpitations, edema, spontaneous sweating, restless fetal movement. Atractylodes macrocephala has a wide range of uses. In addition to traditional Chine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/845
CPCC12P19/04C08B37/0003
Inventor 陈虹张建芬雷超陆胤柯薇
Owner 深圳立专信息科技有限公司
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