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Application of [beta]-myrcene in preparation of medicago sativa drought resistance and growth promotion

A kind of alfalfa, myrcene technology, applied in the application, lawn growth, plant growth regulator and other directions, to achieve the effect of obvious growth promotion effect

Active Publication Date: 2020-08-07
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] At present, the research on the growth-promoting and drought-resistant effects of β-myrcene on plants is still in a blank stage at home and abroad.

Method used

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  • Application of [beta]-myrcene in preparation of medicago sativa drought resistance and growth promotion
  • Application of [beta]-myrcene in preparation of medicago sativa drought resistance and growth promotion
  • Application of [beta]-myrcene in preparation of medicago sativa drought resistance and growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Growth-promoting effect of β-myrcene on alfalfa seedlings in petri dishes:

[0042] β-myrcene was prepared with Tween 20 to a concentration of 10 μl / mL, and then diluted to 5 μl / mL, 2 μl / mL, 1 μl / mL, 0.5 μl / mL and 0.25 μl / mL;

[0043] Place filter paper in a petri dish with a diameter of 9 cm and add 60 alfalfa seeds, then add 5 ml of β- Myrcene solution, each treatment was repeated twice, and used as a blank control of distilled water; sealed with a parafilm, placed in a 25°C incubator for 5 days in the dark, and then measured the seedling height and root length of the seedlings;

[0044] Data statistics method: first calculate the growth rate of the root length and seedling height of alfalfa under each concentration to the blank control: Then use one-way ANOVA to detect whether the difference between the data of each group of the growth rate is significant, and then use the LSD method to analyze the data, and the difference between the groups with different marked l...

Embodiment 2

[0047] Growth-promoting effects of β-myrcene on potted alfalfa under normal and drought-treated conditions:

[0048] Prepare β-myrcene with Tween 20 into 0.1 μl / mL and 0.25 μl / mL solutions, put the solution in a small watering can and mark it, and add distilled water to another small watering can as a blank control;

[0049] When the alfalfa seedlings in the pot grow to about 10cm, start spraying the prepared solutions with concentrations of 0.1μl / mL and 0.25μl / mL, spray once every 3 days, spray 10 times each time, and set 2 for each concentration. A spraying method, that is, spraying 2 times and spraying 4 times, and the potted plants were divided into two groups, one group was watered normally, and the other group was watered in half, and the watering frequency was halved as a drought treatment. After spraying the solution for 20 days, Measure the root length, seedling height and fresh weight of alfalfa, and measure the dry weight after putting fresh alfalfa in an oven for d...

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Abstract

The invention discloses application of [beta]-myrcene in preparation of medicago sativa drought resistance and growth promotion, [beta]-myrcene is prepared into a low-concentration aqueous solution with Tween 20, medicago sativa seeds are cultured in a culture dish, and results show that [beta]-myrcene significantly promotes growth of roots of medicago sativa seedlings. [beta]-myrcene is selected,and Tween 20 is used for preparing an aqueous solution; the [beta]-myrcene is sprayed to medicago sativa seedlings under normal and drought treatment, and results show that the [beta]-myrcene has a promotion effect on medicago sativa under normal growth conditions and has a more remarkable promotion effect on medicago sativa under drought conditions, so that the [beta]-myrcene has an obvious drought resistance and growth promotion effect on medicago sativa. The [beta]-myrcene is a natural product, can be degraded in the nature, is non-toxic and harmless to the environment, can be prepared ona large scale, is low in cost, low in use concentration, small in dosage and strong in effect, and has extremely high application potential.

Description

technical field [0001] The invention relates to the application of a compound to the drought-resistant and growth-promoting effect of forage grass, in particular to the application of β-myrcene in the preparation of alfalfa drought-resistant and growth-promoting effect. Background technique [0002] Alfalfa is an important herbage, and the state has listed the "industrialization of alfalfa" as a scientific and technological project, in order to realize the optimization of my country's planting industry structure and promote the sustainable development of agriculture. As a high-quality variety of alfalfa, alfalfa is an important source of high-quality protein in animal husbandry. Alfalfa is suitable for warm and semi-humid to semi-arid climates, and is mostly distributed in the north of the Yangtze River in my country. In areas with less precipitation, alfalfa is facing the problem of how to resist drought and increase production. The traditional method mainly relies on coup...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01P21/00A01G7/06A01G20/00
CPCA01N27/00A01G7/06A01G20/00
Inventor 邵华石凯姜春宇黄岭周世兴张弛韩彩霞
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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