Reversible syringomyelia animal model, and construction method and application thereof

Abstract

The invention discloses a reversible syringomyelia animal model, and a construction method and application thereof. The construction method comprises the following steps: S1, exposing a T13 vertebralplate, and cutting a ligamentum flavum to expose a spinal dura mater; S2, quantitatively oppressing the T13 spinal dura mater to make cerebrospinal fluid extruded to the periphery and the circulationof the cerebrospinal fluid blocked; and S3, removing the vertebral plate and an oppressor to reduce the pressure in order to make the subdural cerebrospinal fluid recovered again. A quantitative cotton sliver is filled into the inner side of the T13 vertebral plate of a rat through microsurgery, cerebrospinal fluid flow is blocked through epidural oppression to make the rat have syringomyelia, andthen an operation relieves the oppression, reverses syringomyelia, and realizes the gradual reduction of rat syringomyelia. According to the method, an animal model of the syringomyelia caused by reversible non-biochemical destruction is established, feasibility verification of MRI dynamic observation of the animal syringomyelia is achieved, the constructed cavity is a reversible cavity, and a model basis is provided for related research of the syringomyelia and spinal cord injury.

Description

technical field [0001] The present application relates to the technical field of animal model construction, in particular to a reversible syringomyelia animal model and its construction method and application. Background technique [0002] Syringomyelia is a morphological lesion of the spinal cord caused by cerebrospinal fluid dynamic disorders. It is divided into communicating and non-communicating syringomyelia. The former is more common in Chiari malformation, and the latter is more common in spinal cord injuries, tumors, and inflammation. In patients with Chiari malformation, the cerebellar tonsil herniates downward into the foramen magnum, resulting in a relatively narrow foramen magnum, which hinders the circulation of cerebrospinal fluid in the fourth ventricle, the subarachnoid space of the spinal canal, and the central canal of the spinal cord, eventually leading to local enlargement of the central canal of the spinal cord. In spinal cord injury, localized injury le...

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Problems solved by technology

[0004]Previous studies on spinal cord injury were based on spinal cord percussion and transection models. This research method has great limitations: ①The degree of spinal cord injury is severe, and the spinal cord parenchyma and ependyma All areas are severely damaged, endogenous stem cells are severely damaged, and most of the ability to proliferate, differentiate and migrate may be lost

②This model is an acute injury model. Acute inflammatory reaction occurs in the spinal cord, the microenvironment changes suddenly, and the spinal cord nerve cells and stem cells cannot adapt.

③ Spinal cord injury is irreversible, and nerve function cannot be recovered. It is impossible to observe the natural repair process of spinal cord injury in vivo, and it is impossible to dynamically observe the proliferation, differentiation and migration characteristics of spinal cord endogenous neural stem cells.

② Injection of kaolin or quizic acid into the spinal cord parenchyma can induce cavities by destroying the spinal cord parenchyma or causing inflammation, but this method will lead to significant proliferation of inflammatory cells in the spinal cord. Whether inflammation will affect the growth of ENSCs has not been studied. explore

[0006] Generally speaking, there is no animal model that can better simulate human syringomyelia, which seriously restricts the research on syringomyelia and spinal cord injury

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