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Application of BXL gene or encoded protein thereof
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A technology encoding proteins and genes, applied in the field of agronomy, can solve problems such as limited scope of application, violation of green concepts, and limited scope of drought improvement.
Active Publication Date: 2020-08-07
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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However, there are many disadvantages in these conventional means of improving drought, for example, it is difficult to implement, consumes more manpower and material resources, is uneconomical, poor in engineering changeability, limited in the scope of improving drought, and limited in scope of application, etc.
People use the method of spraying pesticides to achieve the improvement of pathogenic bacteria. Although it is direct, there are many shortcomings, such as: pesticide residues, endangering human health, causing environmental pollution, polluting water resources, destroying air cleanliness, and violating the principles advocated by human beings. green concept
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Embodiment 1
[0225] The Arabidopsis models involved in the following examples are Col-0, SALK_012090C(bxl1-1), and SALK_086578C(bxl1-2) from the Arabidopsis Biological Resource Center (ABRC).
[0226] Take Arabidopsis, for example.
[0227] 1. Mutant Acquisition
[0228] From the genome-wide transcript-level sequencing data induced by Bacillus amyloliquefaciens strain GB03 (a commercial model plant-growth-promoting beneficial plant rhizosphere, purchased from ATCC, Global Bioresource Center), a significant Down-regulated gene, beta-xylosidase 1 (BXL1). The seed number of the T-DNA insertion mutant of this gene was found on the Tair website, and two T-DNA insertion mutants, SALK_0120909C and SALK_86578C, were ordered at the Arabidopsis Biological Resource Center (ABRC). Through genotype identification, after confirming that it is correct, it was observed that the BXL1 gene function loss mutant showed delayed germination, slightly slower vegetative growth and delayed flowering time during ...
Embodiment 2
[0236] BXL1 has homologous genes in many dicotyledonous plants, such as alfalfa, apple, flax, rape, radish, pepper, cherry, jujube, water shield and alfalfa. In alfalfa, the gene highly homologous to BXL1 was named Medtr2g034720.1. The invention realizes the expression regulation of Medtr2g034720.1 gene in alfalfa through gene editing technology, so as to change the composition of alfalfa cell wall, adjust the palatability of alfalfa as feed, and enhance the ability of alfalfa to resist biotic and abiotic stress.
[0237] CRISPR (regularly clustered interspaced short palindromic repeats) is a powerful gene editing technology that has been widely used in animals and plants. The experimental design of the present invention selects the psgR-Cas9-At vector, and designs sg-RNA online at www.atum.bio / eCommerce / cas9 / input. In order to effectively construct the target fragment of the present invention on the psgR-Cas9-At vector, we synthesized two forms of oligonucleotide adapters, n...
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Abstract
The invention provides an application of a BXL gene or an encoded protein thereof. Particularly, when the expression of the BXL gene or the encoded protein thereof is inhibited, the traits of a plantcan be remarkably improved, including: (i) enhancing the stress resistance of the plant; and / or (ii) anti-pathogenic bacteria; and / or (iii) reducing lignin content, increasing fiber and pectin content. Furthermore, the invention also finds that the inhibitor of the BXL gene or the encoded protein thereof can also be used for a feed composition for improving the palatability of the feed.
Description
technical field [0001] The invention relates to the field of agronomy, in particular to the application of a BXL gene or its encoded protein. Background technique [0002] Plants experience various biotic or abiotic stresses during their growth. Drought and pathogenic bacteria, as the two main factors that harm plant growth, have been widely focused by people. [0003] However, the current main means of improving drought and pathogenic bacteria are conventional watering techniques and spraying pesticides. Conventional irrigation techniques include: digging ditches, building reservoirs, barrages, water diversion, or the water-saving irrigation technique commonly used in greenhouses --- drip irrigation. However, these conventional means of improving drought have many disadvantages, such as difficulty in implementation, high consumption of manpower and material resources, uneconomical, poor engineering changeability, limited scope for improving drought, limited scope of appli...
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