Primer sets for differential diagnosis of dhav-1, dhav-n and dhbv

Abstract

The present invention provides a primer set for differential diagnosis of DHAV‑1, DHAV‑N and DHBV, which belongs to the field of waterfowl epidemiology. The sequence of the primer set is shown in SEQ ID NO.1‑5, which is obtained by constructing the primer set The diagnostic kit can simultaneously identify three duck hepatitis viruses DHAV‑1, DHAV‑N and DHBV, and the method is quick and easy. At present, there are no relevant research reports at home and abroad, and the establishment of the present invention can fill the gaps in related fields at home and abroad.

Description

technical field [0001] The invention provides a primer set for differential diagnosis of DHAV-1, DHAV-N and DHBV, and belongs to the field of waterfowl infectious diseases. Background technique [0002] In 1945, the first strain of duck viral hepatitis virus (ie duck hepatitis A virus type 1 DHAV-1) was discovered in the Long Island Duck Farm in New York, USA. In 1950, Levine and Fabricant first isolated the pathogen of duck viral hepatitis from chicken embryos, and then many countries in the world reported the prevalence of DHAV-1 one after another. In my country, in 1963 Huang Junjian of the Shanghai Institute of Animal Husbandry first reported that duck viral hepatitis was found in Shanghai. In May 1999, Su Jingliang et al. isolated two strains of the virus (respectively numbered strain B and strain G) from dead ducks suspected of duck hepatitis in Beijing and Guangxi. The duck embryo serum neutralization test showed that the two strains were of the same serotype, but th...

AI Technical Summary

Problems solved by technology

Further studies have confirmed that the morphological structure, nucleic acid composition and biological characteristics of DHBV are similar to human hepatitis B HBV, and DHBV can also cause hepatitis and liver cirrhosis after infecting ducks, causing great harm to the duck industry

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