Application of golden berry extract in preparing composition for improving gene expression and mitochondrial activity

A technology of golden raspberry and extract is applied in the field of golden raspberry extract for preparing a composition for improving gene expression and mitochondrial activity, which can solve the problems of inability to directly and effectively improve, delay skin aging, decrease water retention capacity, and the like, Achieve the effect of maintaining vitality and normal metabolism, preventing skin moisture loss and enhancing moisturizing ability

Pending Publication Date: 2020-08-18
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, most of the skin anti-aging products on the market can only increase the antioxidant activity, and cannot directly and effectively improve or delay the occurrence of skin aging
[0005] Based on the above, in order to improve the skin’s fragility and sensitivity caused by damaged keratinocytes, decreased water retention capacity, and skin aging, it is necessary to develop a method that can directly and effectively improve or delay the occurrence of skin aging, while at the same time A composition that can effectively make keratinocytes secrete more moisturizing factors to maintain the arrangement of keratinocytes and maintain the integrity of the stratum corneum structure is really necessary

Method used

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  • Application of golden berry extract in preparing composition for improving gene expression and mitochondrial activity
  • Application of golden berry extract in preparing composition for improving gene expression and mitochondrial activity
  • Application of golden berry extract in preparing composition for improving gene expression and mitochondrial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 The preparation method of the golden berry extract of the present invention

[0034] In one embodiment of the present invention, after the golden berry fruit is cleaned first, the extraction solvent of the golden berry fruit and water, alcohol, or a mixture of alcohol and water, preferably water, is mixed in a ratio of 1-5:10- Mix at a weight ratio of 20, homogenize and extract in a solvent at 50-100° C. for 0.5-2 hours, cool the crude extract to room temperature, and filter the crude extract with a 400 mesh filter to obtain a filtrate. Finally, the filtrate is concentrated under reduced pressure at 45-70° C. to obtain the golden berry extract of the present invention.

Embodiment 2

[0035] Example 2 The effect of the golden berry extract of the present invention on enhancing the expression of keratinocyte moisturizing genes

[0036] One embodiment of the present invention uses human primary epidermal keratinocytes HPEK-50 (Human primary epidermal keratinocytes-50, HPEK-50) to carry out the golden berry extract of the present invention to enhance the expression of TGM1 gene, KRT10 gene, and KRT14 gene in a short time Quantitative efficacy testing. The HPEK-50 cells were purchased from CELLnTEC (Switzerland), and were cultured in serum-free keratinocyte culture fluid (keratinocyte-SFM), which was purchased from Gibco (USA), No. #17005042, and in Contains 5% CO 2 cultured in a 37°C cell culture incubator.

[0037] First, place the 1.5x10 5 Each HPEK-50 cell was cultured in a 6-well culture dish containing 2 mL of the above-mentioned culture solution in each well, and cultured at 37°C for 16-18 hours, and then the cells were divided into the following thre...

Embodiment 3

[0042] Example 3 The effect of the golden berry extract of the present invention on enhancing the mitochondrial activity of skin cells

[0043] One embodiment of the present invention uses human skin fibroblast (Human skin fibroblast) CCD-966SK cells, and BD TM MitoScreen (JC-1) reagent kit (Flow cytometry Mitochondrial membrane potential detection kit) is used to test the effectiveness of the golden berry extract of the present invention in enhancing the mitochondrial activity of skin cells. Wherein, the CCD-966SK cells were purchased from the American Type Culture Collection (ATCC, U.S.), numbered BCRCNo.60153, and the cells were cultured in 10% fetal bovine serum (Fetal Bovine Serum) and 90% Eagle's The culture solution of Minimum Essential Medium (EMEM) (purchased from Gibco, the U.S.), which contains 0.1mM non-essential amino acids (Non-essential amino acids), 1.5g / L sodium bicarbonate (Sodiumbicarbonate), and 1mM pyruvate Sodium (Sodium pyruvate).

[0044] where, in us...

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Abstract

The invention relates to the field of plant extracts, in particular to application of a golden berry extract in preparing a composition for improving gene expression and mitochondrial activity. The invention provides a golden berry extract, which is obtained by extracting golden berry with a solvent, wherein the solvent is water, alcohol or an alcohol-water mixture. The invention also provides application of the golden berry extract in improving expression of TGM genes and / or KRT genes and mitochondrial activity. The golden berry extract can enable the skin to form more moisturizing factors, maintain the structural integrity of the horny layer so as to improve the barrier function of the skin, and effectively improve the activity of mitochondria so as to maintain the normal metabolism andthe anti-aging activity of the skin.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to the use of golden berry extracts for preparing compositions for improving gene expression and mitochondrial activity. Background technique [0002] The epidermis is the outermost layer of the skin. From the outside to the inside, it consists of the stratum corneum, granular layer, spinous layer, and basal layer. The epidermis is mainly formed by continuous upward differentiation of undifferentiated cylindrical keratinocytes in the basal layer. The process is called keratinization. The water content in corneocytes is high. As the cells metabolize and differentiate upward, the shape of corneocytes will gradually become flat, and the nucleus and organelles will begin to degenerate and shrink, and dead cells without nuclei and organelles will form in the stratum corneum. The main function of the epidermis is to keep the skin water and form a skin barrier to resist various external inju...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A61P17/16A61P17/18A61K8/9789A61Q19/00A61Q19/08A23L33/105A61K131/00
CPCA61K36/81A61P17/16A61P17/18A61K8/9789A61Q19/00A61Q19/08A23L33/105A61K2800/78A23V2002/00A23V2200/318A23V2200/30A23V2250/21
Inventor 林咏翔李姿仪
Owner TCI CO LTD
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