Application of golden berry extract in preparing composition for improving gene expression and mitochondrial activity
A technology of golden raspberry and extract is applied in the field of golden raspberry extract for preparing a composition for improving gene expression and mitochondrial activity, which can solve the problems of inability to directly and effectively improve, delay skin aging, decrease water retention capacity, and the like, Achieve the effect of maintaining vitality and normal metabolism, preventing skin moisture loss and enhancing moisturizing ability
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Embodiment 1
[0033] Embodiment 1 The preparation method of the golden berry extract of the present invention
[0034] In one embodiment of the present invention, after the golden berry fruit is cleaned first, the extraction solvent of the golden berry fruit and water, alcohol, or a mixture of alcohol and water, preferably water, is mixed in a ratio of 1-5:10- Mix at a weight ratio of 20, homogenize and extract in a solvent at 50-100° C. for 0.5-2 hours, cool the crude extract to room temperature, and filter the crude extract with a 400 mesh filter to obtain a filtrate. Finally, the filtrate is concentrated under reduced pressure at 45-70° C. to obtain the golden berry extract of the present invention.
Embodiment 2
[0035] Example 2 The effect of the golden berry extract of the present invention on enhancing the expression of keratinocyte moisturizing genes
[0036] One embodiment of the present invention uses human primary epidermal keratinocytes HPEK-50 (Human primary epidermal keratinocytes-50, HPEK-50) to carry out the golden berry extract of the present invention to enhance the expression of TGM1 gene, KRT10 gene, and KRT14 gene in a short time Quantitative efficacy testing. The HPEK-50 cells were purchased from CELLnTEC (Switzerland), and were cultured in serum-free keratinocyte culture fluid (keratinocyte-SFM), which was purchased from Gibco (USA), No. #17005042, and in Contains 5% CO 2 cultured in a 37°C cell culture incubator.
[0037] First, place the 1.5x10 5 Each HPEK-50 cell was cultured in a 6-well culture dish containing 2 mL of the above-mentioned culture solution in each well, and cultured at 37°C for 16-18 hours, and then the cells were divided into the following thre...
Embodiment 3
[0042] Example 3 The effect of the golden berry extract of the present invention on enhancing the mitochondrial activity of skin cells
[0043] One embodiment of the present invention uses human skin fibroblast (Human skin fibroblast) CCD-966SK cells, and BD TM MitoScreen (JC-1) reagent kit (Flow cytometry Mitochondrial membrane potential detection kit) is used to test the effectiveness of the golden berry extract of the present invention in enhancing the mitochondrial activity of skin cells. Wherein, the CCD-966SK cells were purchased from the American Type Culture Collection (ATCC, U.S.), numbered BCRCNo.60153, and the cells were cultured in 10% fetal bovine serum (Fetal Bovine Serum) and 90% Eagle's The culture solution of Minimum Essential Medium (EMEM) (purchased from Gibco, the U.S.), which contains 0.1mM non-essential amino acids (Non-essential amino acids), 1.5g / L sodium bicarbonate (Sodiumbicarbonate), and 1mM pyruvate Sodium (Sodium pyruvate).
[0044] where, in us...
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