Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof

A technology for microbial fermentation bacteria and wheat straw, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that pathogenic bacteria are difficult to be completely removed, the activity of Fusarium graminearum cannot be inhibited, and the production efficiency of organic fertilizers is affected. and other problems, to achieve the effect of good field application effect, easy large-scale promotion, and shortened production cycle.

Inactive Publication Date: 2020-08-18
ANHUI SCI & TECH UNIV
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Problems solved by technology

In addition, there will be certain toxin residues in its meat, eggs, and milk products, which will cause diffuse harm to consumers.
[0004] What's more serious is that for the wheat infiltrated by Fusarium graminearum, after the wheat is mature and harvested, a large number of pathogenic bacteria remain in the wheat straw, and it is often difficult to completely remove the pathogenic bacteria in the process of composting and fermentation, thus limiting the quality of wheat. Application of organic fertilizer made from straw
[0005

Method used

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  • Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof
  • Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof
  • Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof

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[0038] Example 1: Expanded cultivation of microbial flora

[0039] 1. Pretreatment of straw raw materials: the diseased straws are collected from wheat fields in Nanjing, Jiangsu Province, crushed to powder, air-dried naturally, and set aside;

[0040] 2. Isolation of microbial flora: fill 100mL sterile inorganic salt culture medium into a 250mL Erlenmeyer flask, add straw to the Erlenmeyer flask according to 5% of the liquid volume, culture at 30℃ shaker, 180rpm for 48h, Transfer the bacterial population suspension to a fresh 100 mL sterile inorganic salt medium at 10% of the liquid volume, continue to culture for 48 hours, and subculture 5 times according to the above method;

[0041] 3. Preparation of fermentation broth: Put 400L of inorganic salt medium in a 500L fermentor, sterilize at 121℃, 0.1MPa for 30min, and cool to room temperature;

[0042] 4. Expansion culture of the flora: add the treated straw to the fermentation tank at 5% of the liquid volume, and add the seed solutio...

Example Embodiment

[0044] Example 2: Determination of relevant parameters in the fermentation and composting of diseased straw

[0045] 1. Preparation of Fusarium graminearum spore liquid

[0046] Transfer the activated Fusarium graminearum to a PDA plate, culture for 5 days at 25°C, pick the hyphae and insert them into mung bean culture medium, culture at 25°C, 180rpm, shaker for 5 days, filter with gauze, and centrifuge the filtrate at 7000rpm for 10min. The precipitate is resuspended in sterile water at a concentration of 10 5 Pcs / mL, put in the refrigerator for later use.

[0047] 2. Fermentation of diseased wheat straw

[0048] 1) Experimental setup: There are three groups of treatments, including a control group (natural fermentation of straw) and two experimental groups (respectively using the microbial fermentation inoculum prepared by the present invention and Beijia organic fertilizer fermentation inoculum to treat diseased straws). The degree of fermentation maturity of the straw in the same...

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Abstract

The invention discloses a microbial fermentation inoculant for accelerating wheat straw fermentation and a preparation method of the microbial fermentation inoculant. The microbial agent is obtained by carrying out enlarged culture on microbial florae separated from infected straws, wherein the microbial flora mainly comprises two major components, namely bacteria and actinomycetes, and the identified bacterial components comprise bacillus, pseudomonas, pantoea, bifidobacterium, bacillus aceticus, lactobacillus, stenotrophomonas, klebsiella, citrobacter and flavobacterium. The microbial agentprepared by the invention has a very high prevention effect on wheat scab and gibberellic toxin, can play a role in inhibiting the growth of pathogenic bacteria in the fermentation process of wheat straw raw materials for preparing organic fertilizers, can also accelerate the fermentation and decomposition speed of straws, and is remarkable in effect and strong in universality.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculum, and in particular relates to a microbial fermentation inoculum for accelerating wheat straw fermentation and a preparation method thereof. Background technique [0002] Straw is rich in organic matter and a variety of nutrients needed for crop growth, and is an important raw material for fermentation into organic fertilizers. The principle of straw fermentation is that during the fermentation process, the organic matter in straw will undergo a complex transformation process under the action of microorganisms. First, the complex organic matter will be decomposed into simple substances, and finally carbon dioxide, water and mineral nutrients will be generated; followed by organic matter The resynthesis of organic humus is more complex, which is more conducive to the absorption and utilization of crops. Fermentation of straw to make organic fertilizer, on the one hand, can stop the burni...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/80C05F17/20C05F11/00C12R1/07C12R1/38C12R1/01C12R1/02C12R1/225C12R1/22C12R1/20
CPCC05F11/00C12N1/20C05F17/20C05F17/80Y02W30/40
Inventor 杨洪杏王翔马婷婷赵建荣张浩周椿富曹胜飞项进韩振鲍文梅程嘉蓉程嘉慧
Owner ANHUI SCI & TECH UNIV
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