Difunctional antigen guided sildenafil and tadalafil antibody array detection card

A technology of sildenafil and tadalafil, applied in the field of antibody array detection cards of sildenafil and tadalafil, can solve the problems of unstable quality, low sensitivity, weak detection signal strength, etc. Detection sensitivity and quantitative precision, the effect of improving detection signal strength and sensitivity, and increasing signal strength and stability

Pending Publication Date: 2020-08-21
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: 1. To detect different target substances, it is necessary to use corresponding detection antibodies to connect quantum dot microspheres to prepare fluorescent probes, which requires a large workload, low yield, and unstable quality; The reaction to prepare fluorescent probes may affect the structure and immunoaffinity of antibodies; 3. Lack of signal amplification mechanism, the detection signal intensity is weak and the sensitivity is low
However, the immunogenicity of the "detection antigen carrier protein" is unstable, and slight changes in protein conformation will lead to a decrease in the affinity between the "universal antibody" and the "detection antigen", weaken the formation of "sandwich immune complexes", and affect the detection signal. produce

Method used

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  • Difunctional antigen guided sildenafil and tadalafil antibody array detection card
  • Difunctional antigen guided sildenafil and tadalafil antibody array detection card
  • Difunctional antigen guided sildenafil and tadalafil antibody array detection card

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A dual-functional antigen-guided antibody array detection card for sildenafil and tadalafil, comprising a liner 5 and bifunctional antigen-releasing pads that are sequentially adhered to the liner 5 and partially overlapped adjacent to each other 1. Universal fluorescent probe release pad 2, nitrocellulose membrane 3 (chromatographic membrane) and water absorbent pad 4; On the basis of the detection antigen formed by coupling "7-amino-4-hydroxy-2-naphthalenesulfonic acid" label 62; the universal fluorescent probe release pad 2 contains anti-"7-amino-4- The universal fluorescent probe of hydroxy-2-naphthalenesulfonic acid "antibody 65, the universal fluorescent probe is quantum dot 61; The nitrocellulose membrane 3 is provided with detection line A, detection line B and quality control line 33, so The detection line A and detection line B are respectively immobilized anti-sildenafil antibody 311 and anti-tadalafil antibody 321, and the quality control line 33 is immobili...

Embodiment 2

[0050] Simultaneously detect the detection method of sildenafil and tadalafil, the steps are as follows:

[0051]Take 0.2g (or 0.2mL) sample, dissolve it in 2.0mL absolute ethanol to fully extract the target sample, let the extract stand for 10 minutes to fully precipitate impurities, take 0.2mL supernatant and add it to 20mL sample buffer, fully Shake well to form a sample solution; drop 3-4 drops of sample solution on the bifunctional antigen release pad with a dropper, and the bifunctional antigen and universal fluorescent probe are released during the movement of the sample solution to the nitrocellulose membrane, and pass through the detection area successively. Line and quality control line, according to the fluorescent signal of detection line A, detection line B and quality control line to determine whether the sample contains sildenafil and tadalafil.

[0052] refer to Figure 5 . The anti-sildenafil antibody 311 immobilized on the nitrocellulose membrane detection ...

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Abstract

The invention relates to a difunctional antigen guided sildenafil and tadalafil antibody array detection card. The detection card comprises a lining plate, and a difunctional antigen release pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and are partially overlapped with one another, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by coupling ovalbumin with sildenafil and tadalafil respectively; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with a detection line A, a detection line B anda quality control line which are respectively used for fixing an anti-sildenafil antibody, an anti-tadalafil antibody and the anti-specific marker antibody. According to the invention, the productionof all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, and the quality control reliability is improved; and the signal intensity and the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to an antibody array detection card for sildenafil and tadalafil guided by a bifunctional antigen. Background technique [0002] The common names of sildenafil and tadalafil are "Viagra" and "Super Viagra" respectively, both of which belong to phosphodiesterase-5 (PDE-5) inhibitor drugs, which are popular in the world for treating impotence and aphrodisiac. Because these two effects are obvious and the price of imitation is low, unscrupulous manufacturers illegally add sildenafil and tadalafil to aphrodisiac health food (mainly health wine) to enhance the curative effect and seek illegitimate benefits. The two PDE-5 inhibitor drugs are prescription drugs that can cause a range of side effects such as headaches, flushing, indigestion, blurred vision and muscle aches. Adverse drug reaction information published on the official website of the FDA in the United States show...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/94G01N33/582G01N33/588G01N33/558
Inventor郭杰标
OwnerSHAOGUAN COLLEGE