Application of polypeptide in preparing medicine for treating pancreatitis and medicine containing polypeptide

A technology for pancreatitis and acute pancreatitis, which is applied in the field of medicine to achieve the effects of low immunogenicity, small molecular weight of polypeptides and wide application prospects

Active Publication Date: 2020-08-25
LINK HEALTH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ERG15 is a polypeptide derived from TDL23, consisting of amino acids 5-19 of TDL23, and cERG15 is formed by adding a Cys cycliza

Method used

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  • Application of polypeptide in preparing medicine for treating pancreatitis and medicine containing polypeptide
  • Application of polypeptide in preparing medicine for treating pancreatitis and medicine containing polypeptide
  • Application of polypeptide in preparing medicine for treating pancreatitis and medicine containing polypeptide

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, the synthesis of polypeptide

[0035] Polypeptide TDL23, ERG15 and cERG15 of the present invention are synthesized by solid-phase synthesis process, with a purity >95%, and the HPLC spectra of TDL23, ERG15 and cERG15 are respectively Figure 1A , Figure 1B and Figure 1C , Synthesize 500mg.

Embodiment 2

[0036] Example 2. Therapeutic effect of TDL23 polypeptide on acute pancreatitis in rats

[0037] Modeling: 96 male SD rats of SPF grade, weighing 230-260 g. The SD rats in each group were anesthetized, skin-prepared, and disinfected. A 2-3 cm transverse incision was made into the abdomen at 1-2 cm below the xiphoid process in the middle of the upper abdomen. Determine the liver, stomach, and spleen, find the duodenum by following the stomach, find the pancreas between the duodenal mesentery and the spleen at the back of the stomach, and find the opening of the transparent bile-pancreatic duct and the duodenum at the opening along the inner side of the duodenum. Intestinal big nipples. The common hepatic duct was ligated with an arterial clip near the hilum, and then the needle was advanced obliquely from the side of the duodenum and the direction of the needle was adjusted so that it could enter the bile-pancreatic duct. Slowly inject 2.5% sodium taurocholate 1 mL / kg retrograd...

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Abstract

The invention discloses application of an MEPE-derived polypeptide in preparing a medicine for treating pancreatitis and a medicine containing the MEPE-derived polypeptide. The MEPE-derived polypeptide is: a) a polypeptide represented by SEQ ID NO:1; or b) a polypeptide composed of amino acids at positions 5-19 in the amino acid sequence of the polypeptide represented by SEQ ID NO:1; or c) a cyclic peptide obtained by cyclization by adding Cys respectively to the N-terminal and C-terminal of the polypeptide composed of the amino acids at positions 5-19 in the amino acid sequence of the polypeptide represented by SEQ ID NO:1. The polypeptide provided by the invention has small molecular weight, is easy to synthesize, has low immunogenicity, has a good therapeutic effect on acute pancreatitis, can be used as a new type of medicine for acute pancreatitis, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the use of MEPE-derived polypeptides in preparing medicines for treating pancreatitis and medicines containing them. Background technique [0002] TDL23 is a peptide containing 23 amino acids derived from extracellular matrix phosphoglycoprotein (MEPE). It has been reported that TDL23 can promote the formation of bone, cartilage, and dental bone, and can be used to treat bone and cartilage-related diseases (see Patent Document 1 , 2; non-patent literature 1, 2, 3), but there is no report that TDL23 is used for the treatment of pancreatitis. ERG15 is a polypeptide derived from TDL23, consisting of amino acids 5-19 of TDL23, and cERG15 is formed by adding a Cys cyclization to the N-terminus and C-terminus of ERG15, and ERG15 and cERG15 are also not used for the treatment of pancreatitis reports. [0003] prior art literature [0004] patent documents [0005] Patent...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K38/10A61K38/12A61K9/08A61K9/19A61P1/18
CPCA61K38/16A61K38/10A61K38/12A61K9/08A61K9/19A61P1/18Y02A50/30
Inventor 许元生卢肖宇李天然孟鹤王华
Owner LINK HEALTH GRP
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