Process for separation of gallated epicatechins (egcg & ecg) from green tea extract or green tea dust

A technology of green tea extract and epicatechin, applied in the field of hydrated gallated epicatechin-isonicotinic acid complex, can solve undisclosed problems

Pending Publication Date: 2020-08-28
克里斯托莫菲克斯技术私人有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the prior art references disclose the use of isonicotinic acid for the isolation of the gallate forms of epicatechin (EGCG and ECG) from green tea extract or green tea powder and the formation of the hydrated gallate form from green tea extract or green tea powder. Epicatechin (EGCG&ECG)-Isonicotinic Acid Complex

Method used

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  • Process for separation of gallated epicatechins (egcg & ecg) from green tea extract or green tea dust
  • Process for separation of gallated epicatechins (egcg & ecg) from green tea extract or green tea dust
  • Process for separation of gallated epicatechins (egcg & ecg) from green tea extract or green tea dust

Examples

Experimental program
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Effect test

example 1

[0129] Step 1: Preparation of hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex:

[0130]10 grams of green tea extract (>50% EGCG and >90% polyphenols) were dissolved in 80 ml water at room temperature. To this clear solution, 3.02 grams of isonicotinic acid was added, precipitation was observed after one hour, and slurrying was continued for 12 hours. The hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex was filtered and the wet cake was washed with 80 ml of water and blotted dry. The hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex was ground using a mortar and pestle to obtain 8.7 g of fine powder.

[0131] Step 2: Breakdown of hydrated epicatechin (EGCG and ECG)-isonicotinic acid complexes to release gallated epicatechin

[0132] 42.5 ml of ethanol was added to 8.5 g of hydrated gallated epicatechin (EGCG & ECG)-isonicotinic acid complex and the suspension was slurried at 45°C for 1 hour. Filter the precipi...

example 2

[0134] Step-1 Preparation of hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex:

[0135] 10 grams of green tea extract (>50% EGCG and >90% polyphenols) were dissolved in 80 ml water at room temperature. To this clear solution, 3.02 grams of isonicotinic acid was added, precipitation was observed after one hour, and slurrying was continued for 12 hours. The hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex was filtered and the wet cake was washed with 80 ml of water and blotted dry. The hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex was ground using a mortar and pestle to obtain 8.7 g of fine powder.

[0136] Step 2: Breakdown of hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex to release gallated epicatechin

[0137] 42.5 ml of acetone was added to 8.5 g of hydrated gallated epicatechin (EGCG & ECG)-isonicotinic acid complex and the suspension was slurried at 45°C for 1 hour. The precipi...

example 3

[0140] Step 1: Process for obtaining crude hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complexes

[0141] 60 g of green tea powder were extracted with 450 ml of aqueous acetone (acetone:water ratio 2:1) (tea:solvent ratio 1:7.5) at 50° C. for 3 hours. Insolubles were filtered off. The upper green tea extract solution was concentrated to 90 ml at 40°C under reduced pressure, and then the concentrated aqueous solution was centrifuged at 25°C for 10 min (7000 rpm) to remove any insoluble matter. 3.999 g of isonicotinic acid and 0.540 g of hydrated gallated epicatechin (EGCG & ECG)-isonicotinic acid complex seed crystals were added to the above clear solution, precipitation was observed after one hour and the slurry was continued for 12 hours. The hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex was filtered and the wet cake was washed with 90 ml of water and blotted dry. The crude compound was ground using a mortar and pestle to obtain 9...

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Abstract

The present invention relates to an improved process for the separation of gallated epicatechins (EGCG & ECG) from green tea extract or green tea dust. The present invention specifically relates to animproved process for the separation of gallated epicatechins (EGCG & ECG) from green tea extract or green tea dust by forming hydrated gallated epicatechins (EGCG & ECG)-isonicotinic acid complex using isonicotinic acid as acid, organic solvents for liberating gallated epicatechins. The present invention more specifically relates to the process for the separation of gallated epicatechins (EGCG &ECG) from green tea extract or green tea dust, wherein processes comprises steps of dissolving, concentrating by centrifugation, adding, precipitating, filtering, slurrying, removing supernatant liquid, washing, extracting and freeze drying. The present invention also relates to hydrated gallated epicatechins (EGCG &ECG)-isonicotinic acid complex.

Description

technical field [0001] The present invention relates to an improved method for the isolation of gallated epicatechin (EGCG and ECG) from green tea extract or green tea powder. [0002] The present invention more particularly relates to an improved method for isolating gallated epicatechin (EGCG and ECG) from green tea extract or green tea powder. [0003] In particular the present invention relates to an improved method for the isolation of gallated epicatechin (EGCG and ECG) from green tea extract or green tea powder by using isonicotinic acid as acid, organic solvent for release Gallated epicatechin to form hydrated gallated epicatechin (EGCG and ECG)-isonicotinic acid complex. [0004] The present invention more particularly relates to a method for isolating gallated epicatechin (EGCG and ECG) from green tea extract or green tea powder, wherein the method comprises dissolution, concentration by centrifugation, addition, precipitation, filtration, slurrying , the steps of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/00A61K31/00
CPCC07D213/79C07D311/62A61K36/82A61K31/353A61K2236/333A61K2236/51
Inventor 瓦姆西·克里希纳·穆皮迪撒迪亚纳瑞亚纳·钦塔
Owner 克里斯托莫菲克斯技术私人有限公司
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