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Full-field structured light coherence coding tomography device and method

A technology of coherent coding and tomography, which is applied in the direction of using optical devices, measuring devices, and material analysis by optical means, can solve the problems of multiple scattered light affecting the signal-to-noise ratio, etc., to shorten the measurement time and reduce the high resolution. Imaging, compact results

Active Publication Date: 2020-09-01
NANJING UNIV OF SCI & TECH
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Problems solved by technology

[0006] The full-field optical coherence tomography method has brought new changes to label-free optical microscopy, but the large numerical aperture causes a series of related signal attenuation and multiple scattered light in biological tissues to affect the signal-to-noise ratio.

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  • Full-field structured light coherence coding tomography device and method
  • Full-field structured light coherence coding tomography device and method
  • Full-field structured light coherence coding tomography device and method

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Embodiment

[0054] Such as figure 1 As shown, a full-field optical coherence tomography device using a structured Kohler illumination system according to the present invention includes: a structured Kohler illumination system 1, a dichroic prism 2, a pair of identical microscopic objective lenses 3, 6, and reference Mirror 4, piezoelectric ceramic 5, sample 7, three-axis displacement platform 8, imaging lens 9, area array camera 10. 1. The structured Kohler lighting system can adopt two optical paths of 11-18 or 19-26, the first scheme Medium light source 11, lenses 12, 15, 18, diaphragms 13, 14, digital micromirror array 16, total reflection lens 17; light source 19 in the second scheme, lenses 20, 23, 26, diaphragms 21, 25, light splitting Prism 22, digital micromirror array 24. The light sources 11 and 19 may be halogen light sources, white light sources, light emitting diodes, supercontinuum light sources or laser light sources.

[0055] figure 2 It is a schematic diagram of the s...

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Abstract

The invention discloses a full-field structured light coherence coding tomography device and method. The device comprises a structured Kohler illumination system, a beam splitter prism, a first microscope objective, a second microscope objective, a reference mirror, piezoelectric ceramics, a sample, a three-axis displacement platform, an imaging lens and an area-array camera. The method comprisesthe following steps: dividing convergent light emitted by the structured light Kohler illumination system into two identical beams of light by the beam splitter prism, wherein the two beams of light respectively enter the rear focal planes of the pair of identical first and second micro-objective lenses, and uniform structure illumination with adjustable field of view and light intensity is formedon the surfaces of the reference lens and the sample; arranging the sample on the three-axis displacement platform, sample light returned from the sample and reference light returned from the reference mirror interfere at the beam splitter prism, and focusing interference light on the area-array camera through the imaging lens. The method has the advantages that the parallel imaging resolution ishigh, the illumination mode is easy to program, the algorithm is easy to transplant, the measurement time is short, and the sample category is not limited.

Description

technical field [0001] The invention relates to the technical field of full-field optical coherence tomography, in particular to a full-field structured light coherent coding tomography device and method. Background technique [0002] Optical coherence tomography (Optical Coherence Tomography, OCT) has gradually attracted the attention of the field of clinical medicine after it was proposed by Huang et al. OCT uses the weak coherent interference between the backscattered light of biological tissue and the reference light to obtain the depth information of biological tissue, and combines scanning imaging to realize micron-scale tomographic imaging of the three-dimensional structure of biological tissue. In order to improve the imaging speed and spatial resolution, Beaurepaire et al. proposed the Full-field Optical Coherence Microscopy (FF- OCM) [2]. FF-OCM uses a halogen lamp as a white light source to achieve high axial resolution, uses a large numerical aperture objective...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/23G01B9/02
CPCG01N21/23G01B9/02091
Inventor 朱越高万荣
Owner NANJING UNIV OF SCI & TECH
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